Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Peter J. Sherwood"'
Analysis of nonideality: insights from high concentration simulations of sedimentation velocity data
Publikováno v:
European Biophysics Journal
The Aviv fluorescence detection system (Aviv-FDS) has allowed the performance of sedimentation velocity experiments on therapeutic antibodies in highly concentrated environments like formulation buffers and serum. Methods were implemented in the soft
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4aec45128cd4d8ea39dae98aef8c8596
https://pubmed.ncbi.nlm.nih.gov/33159218
https://pubmed.ncbi.nlm.nih.gov/33159218
Publikováno v:
Analytical Biochemistry. 550:72-83
The preclinical characterization of biopharmaceuticals seeks to determine the stability, state of aggregation, and interaction of the antibody/drug with other macromolecules in serum. Analytical ultracentrifugation is the best experimental method to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf928440640db887686149ebff465381
https://doi.org/10.1016/j.ab.2018.04.002
https://doi.org/10.1016/j.ab.2018.04.002
Autor:
Wolfgang Peukert, Simon E. Wawra, Ana-Sunčana Smith, Thaseem Thajudeen, Anja Maria Wagemans, Walter F. Stafford, Tobias Guckeisen, Johannes Walter, Maximilian J. Uttinger, Andreas Bear, Peter J. Sherwood
Publikováno v:
Langmuir
Brownian dynamics (BD) has been applied as a comprehensive tool to model sedimentation and diffusion of nanoparticles in analytical ultracentrifugation (AUC) experiments. In this article, we extend the BD algorithm by considering space-dependent diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8745be9cf9eec067d2265192619de09
http://fulir.irb.hr/5369/1/1050442.la9b01916_si_001.pdf
http://fulir.irb.hr/5369/1/1050442.la9b01916_si_001.pdf
Publikováno v:
European biophysics journal : EBJ. 47(7)
The goal of this work is to develop a preclinical method for quantitative hydrodynamic and thermodynamic analysis of therapeutic proteins in crowded environments like human serum. The method utilizes tracer amounts of fluorescently labeled monoclonal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a6e076a9fcf50d248fbf1c9ae762f4b
https://pubmed.ncbi.nlm.nih.gov/30003300
https://pubmed.ncbi.nlm.nih.gov/30003300
Autor:
Wolfgang Peukert, Wei Lin, Johannes Walter, Peter J. Sherwood, Walter F. Stafford, Doris Segets
Publikováno v:
Analytical Chemistry. 87:3396-3403
Analytical ultracentrifugation (AUC) has proven to be a powerful tool for the study of particle size distributions, particle shapes, and interactions with high accuracy and unrevealed resolution. In this work we show how the analysis of sedimentation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b84a18d587d68596221bc7114638a257
https://doi.org/10.1021/ac504649c
https://doi.org/10.1021/ac504649c
Publikováno v:
Biophysical Journal. 114:64a-65a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b05c2dd1e9b7aefe81aa0e5969861519
https://doi.org/10.1016/j.bpj.2017.11.401
https://doi.org/10.1016/j.bpj.2017.11.401
Publikováno v:
Biophysical Journal. 110(3)
There is a long tradition in analytical ultracentrifugation (AUC) to perform simulation studies to investigate the utility of new data analysis methods or to verify the reliability of established approaches to complex systems. Here we compare the use
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f0830ba9ddcb6866d4a63b55052bbd7
Publikováno v:
Analytical Ultracentrifugation ISBN: 9784431559832
The development of the fluorescence detection system (Aviv-FDS) for the AUC allows a single fluorescently labeled species to be quantitatively characterized against a highly concentrated and heterogeneous background. During our use of the FDS to char
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::33c6c77ea7dde6cb9e3894b1617e157e
https://doi.org/10.1007/978-4-431-55985-6_24
https://doi.org/10.1007/978-4-431-55985-6_24
Autor:
Wolfgang Peukert, Thaseem Thajudeen, Johannes Walter, Peter J. Sherwood, Doris Segets, Walter F. Stafford, Thomas J. Nacken
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4140f2fca8c6f0ed925479d4e8ec383
https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&origin=inward&scp=85008222774
https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&origin=inward&scp=85008222774
Autor:
Walter F. Stafford, Peter J. Sherwood
Publikováno v:
Analytical Ultracentrifugation ISBN: 9784431559832
Algorithms have been developed for the analysis of sedimentation equilibrium data by fitting to arbitrary reaction schemes. These have been implemented within the framework of a larger program called Sedanal, which until inclusion of equations for fi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3dd3d4f1d86f39231b885e081b323e0c
https://doi.org/10.1007/978-4-431-55985-6_7
https://doi.org/10.1007/978-4-431-55985-6_7