Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Peter J. Sheffield"'
Autor:
Jeffrey Carl Braman, Peter J Sheffield
Publikováno v:
PLoS ONE, Vol 14, Iss 6, p e0199653 (2019)
A new method is introduced allowing seamless assembly of independent, functionally tested, blunt-end double strand nucleic acid parts (DNA fragments not supplied in vectors such as plasmids) into more complex biological devices (e.g. protein expressi
Externí odkaz:
https://doaj.org/article/5fe7ede3687d4a3eaffe86a5288bfe9d
A new method is described for the seamless assembly of independent, prefabricated and functionally tested blunt-end, double strand nucleic acidparts(DNA fragments) into more complex biologicaldevices(vectors) and higher ordermulti-devicesystems.Indiv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cacb3afb9a771568a336012c638901bd
Autor:
Peter J. Sheffield, Denise Rhodes, Katherine A. Felts, Vivian Zhang, Rebecca Mullinax, Nancy McKinney, Carsten P. Carstens, Sarah E. Johns
Publikováno v:
Synthetic Biology ISBN: 9781493977949
Vector construction and gene cloning are ubiquitous techniques essential to all fields of biological and medical research. They are the first steps in many endeavors leading to expressing proteins to understand gene function and regulation. However,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::61ae94c1e0f8391f07fe5d7271ee3400
https://doi.org/10.1007/978-1-4939-7795-6_27
https://doi.org/10.1007/978-1-4939-7795-6_27
Autor:
Michael J. Ellison, Lei Li, John J.M. Bergeron, David W. Speicher, Marina Hincapie, Sjoerd van der Post, Peter J. Sheffield, Catherine C. L. Wong, Thomas A. Beardslee, P. D. Semchuk, Sean C. Bendall, Daniel Cociorva, Wantao Ying, Caroline May, Seul Ki Jeong, Wei Jia, Philip C. Andrews, Steve A. Carr, Benoit Houle, Robert L. Moritz, Lennart Martens, Ana Lopez-Campistrous, Martin Eisenacher, Alexander W. Bell, Raju Kucherlapati, Ron Beavis, Xiaohong Qian, Fuchu He, Brian L. Hood, John R. Yates, Tommy Nilsson, Kieran Wynne, Radoslav Goldman, Vivian Nguyen, Katy Williams, Giuliano Elia, Rudolf Grimm, Salvatore Sechi, Christoph H. Borchers, Yanming An, Masanori Fujimoto, Catherine E. Au, Young Ki Paik, Mahbod Hajivandi, Peipei Ping, Yun Cai, Pavel Metalnikov, Christine A. Miller, Christian Stephan, Wenhong Zhu, Rushdy Ahmad, Marshall Pope, Jinglan Wang, Thomas P. Conrads, Eric W. Deutsch, Min-Seok Kwon, Gilles A. Lajoie, Michael J. Dunn, Guy G. Poirier, Juan Antonio Vizcaíno, William L. Bigbee, Lina Song, John R. Strahler, Sang Yun Cho, Phillip Gray, Craig A. Dorschel, William S. Hancock, Tony Pawson, Kenneth C. Parker, Kaye D. Speicher, Angela K. Walker, Paul F. Predki, Heather Patsiouras, Richard J. Simpson, Thomas Chappell, Kenneth E. Hung, Qing Zhang, Samir M. Hanash, Eugene A. Kapp, Yueju Wang, Katrin Marcus, Jason J. Struthers, Gavin Meredith, Junying Wei, Yangjun Zhang, Sylvie Bourassa, Elisabet Carlsohn, Jayson A. Falkner, Derek Smith, An Sung Chung, Sylvie Laboissiere, Helmut E. Meyer, Hong Wang, Jeffrey W. Smith, Hyoung Joo Lee, David A. Sarracino, Elmar Langenfeld, Kazuyuki Nakamura, Robert E. Kearney, Bing Yang, Bryan Krastins, Majlinda Kullolli
Publikováno v:
Nature methods
Biochemistry Publications
Biochemistry Publications
We carried out a test sample study to try to identify errors leading to irreproducibility, including incompleteness of peptide sampling, in LC-MS-based proteomics. We distributed a test sample consisting of an equimolar mix of 20 highly purified reco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4b5e903d9691deb1dd901076a5b4974
https://doi.org/10.1038/nmeth.1333
https://doi.org/10.1038/nmeth.1333
Autor:
Mike Pyne, Paul B. Robbins, Robert R. Becklin, Kartika Widjaja, Shuping Lai, Sam Thompson, Lawrence Weir, J. Jay Boniface, Yew-Seng J. Ho, John M. Peltier, Moritz von Rechenberg, Cindy Lou Chepanoske, Peter J. Sheffield, Srdjan Askovic, Yuejun Zhen, Justin Savage, Vladimir Kery, Bonnie Richardson, Nafei Xu, Tahmina Mujtaba
Publikováno v:
International Journal of Mass Spectrometry. 238:119-130
Proteomics-based technologies have the potential to accelerate the development of drugs, but such technologies must be well integrated in order to have a positive impact. We describe, herein, a multi-step process for the discovery of protein–protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9666b69be07da92afc0c60bb54db68e8
https://doi.org/10.1016/j.ijms.2003.12.039
https://doi.org/10.1016/j.ijms.2003.12.039
Autor:
David H. Sherman, Yancho Devedjiev, Peter J. Sheffield, Filip Jelen, Urszula Derewenda, Min He, Jacek Otlewski, Zbigniew Dauter, T.W. Martin, Zygmunt S. Derewenda
Publikováno v:
Structure. 10:933-942
Mitomycin C (MC) is a potent anticancer agent. Streptomyces lavendulae , which produces MC, protects itself from the lethal effects of the drug by expressing several resistance proteins. One of them (MRD) binds MC and functions as a drug exporter. We
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a74a9620e6e4e626ab3f857123d9711
https://doi.org/10.1016/s0969-2126(02)00778-5
https://doi.org/10.1016/s0969-2126(02)00778-5
Autor:
Makoto Noda, Timothy A.J. Haystead, Richard R. Perlungher, Gerard Joberty, Peter J. Sheffield, Ian G. Macara, Makoto Kinoshita
Publikováno v:
Nature Cell Biology. 3:861-866
The Cdc42 GTPase binds to numerous effector proteins that control cell polarity, cytoskeletal remodelling and vesicle transport. In many cases the signalling pathways downstream of these effectors are not known. Here we show that the Cdc42 effectors
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90bead9ee27f7b6285f004133839cc73
https://doi.org/10.1038/ncb1001-861
https://doi.org/10.1038/ncb1001-861
Autor:
Zygmunt S. Derewenda, Sarah M. Garrard, Mary E. Lewis, Kenton L. Longenecker, Peter J. Sheffield
Publikováno v:
Protein Expression and Purification. 21:412-416
Lsc-homology domains are found in several eukaryotic nucleotide exchange factors which act on Rho-family GTPases. They show limited amino acid sequence similarity to RGS proteins, which down-regulate the cellular signaling by the α-subunits of trime
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72c51dcaa7e3fb1eef3b5896ebedc13d
https://doi.org/10.1006/prep.2001.1392
https://doi.org/10.1006/prep.2001.1392
Autor:
Junken Aoki, T.W.P. McMullen, Peter J. Sheffield, Hiroyuki Arai, Keizo Inoue, J. Li, T.W. Martin, Zygmunt S. Derewenda
Publikováno v:
Scopus-Elsevier
The mammalian brain contains significant amounts of the cytosolic isoform Ib of the platelet-activating factor acetylhydrolase (PAF-AH), a unique type of PLA2. This oligomeric protein complex contains three types of subunits: two homologous (63% iden
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::696a48d104ea464905df0c10deba976d
https://doi.org/10.1093/protein/13.12.865
https://doi.org/10.1093/protein/13.12.865
Autor:
Orly Reiner, Junken Aoki, Keizo Inoue, Beat Suter, Sarah M. Garrard, Peter J. Sheffield, Urszula Derewenda, Zygmunt S. Derewenda, Hiroyuki Arai, Michal Caspi
Publikováno v:
Proteins: Structure, Function, and Genetics. 39:1-8
The mammalian intracellular brain platelet-activating factor acetylhydrolase, implicated in the development of cerebral cortex, is a member of the phospholipase A2 superfamily. It is made up of a homodimer of the 45 kDa LIS1 protein (a product of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5f9e4f5c8d21d1e70b6a1bec59b03a40
https://doi.org/10.1002/(sici)1097-0134(20000401)39:1<1::aid-prot1>3.0.co
https://doi.org/10.1002/(sici)1097-0134(20000401)39:1<1::aid-prot1>3.0.co