Zobrazeno 1 - 10 of 44 pro vyhledávání: '"Peter J. Belshaw"'
1
Ion–ion reactions with fixed-charge modified proteins to produce ions in a single, very high charge state
Autor: Lloyd M. Smith, Casey J. Krusemark, Brian L. Frey, Joshua J. Coon, Aaron R. Ledvina, Peter J. Belshaw
Publikováno v: International Journal of Mass Spectrometry. 276:136-143
Electrospray ionization (ESI) of denatured proteins produces a mass spectrum with a broad distribution of multiply charged ions. Attaching fixed positive charges, specifically quaternary ammonium groups, to proteins at their carboxylic acid groups ge
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98409f423850586a3dd23cb5bdc36ac5
https://doi.org/10.1016/j.ijms.2008.07.029
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2
Global Amine and Acid Functional Group Modification of Proteins
Autor: Neil L. Kelleher, Craig D. Wenger, Casey J. Krusemark, Peter J. Belshaw, Jonathan T. Ferguson
Publikováno v: Analytical Chemistry. 80:713-720
A sequential reaction methodology is employed for the complete derivatization of protein thiols, amines, and acids in high purity under denaturing conditions. Following standard thiol alkylation, protein amines are modified via reductive methylation
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7b12f778cc23d901a5d54e16be3d394
https://doi.org/10.1021/ac7019317
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3
Ionizable Isotopic Labeling Reagent for Relative Quantification of Amine Metabolites by Mass Spectrometry
Autor: Shane M. Lamos, Peter J. Belshaw, Brian L. Frey, Lloyd M. Smith, Margaret F. Phillips, Michael R. Shortreed, Madhusudan Patel
Publikováno v: Analytical Chemistry. 78:6398-6403
A powerful approach to relative quantification by mass spectrometry is to employ labeling reagents that target specific functional groups in molecules of interest. A quantitative comparison of two or more samples may be readily accomplished by using
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f0c8ea74dc82cf8aee39a40a4d0e6a0
https://doi.org/10.1021/ac0607008
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5
Progress in gene assembly from a MAS-driven DNA microarray
Autor: K. Heinrich, Michael R. Sussman, Matthew J. Rodesch, Mo-Huang Li, Changhan Kim, Kathryn E. Richmond, J. Kaysen, Franco Cerrina, S. Blair, Peter J. Belshaw, L. Chu, Brock F. Binkowski
Publikováno v: Microelectronic Engineering. 83:1613-1616
We report the progress on 765bp full green fluorescent protein (GFP) gene assembly of oligonucleotides from a single DNA microarray, using the amplification and assembly of chip-eluted DNA (AACED) strategy. The chip was fabricated with maskless array
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::315f882f9bd4e45e2279dec1c3e37a0b
https://doi.org/10.1016/j.mee.2006.01.143
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6
Ligand-Regulated Peptides: A General Approach for Modulating Protein-Peptide Interactions with Small Molecules
Autor: Peter J. Belshaw, Brock F. Binkowski, Russell A. Miller
Publikováno v: Chemistry & Biology. 12(7):847-855
SummaryWe engineered a novel ligand-regulated peptide (LiRP) system where the binding activity of intracellular peptides is controlled by a cell-permeable small molecule. In the absence of ligand, peptides expressed as fusions in an FKBP-peptide-FRB-
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcf1e636e652b213dd9bc255fd958c48
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7
Exo-Mechanism Proximity-Accelerated Alkylations: Investigations of Linkers, Electrophiles and Surface Mutations in Engineered Cyclophilin-Cyclosporin Systems
Autor: Melissa D. Boersma, Christopher J. Ciolli, Peter J. Belshaw, Konstantin Levitsky
Publikováno v: ChemBioChem. 6:890-899
Investigations on the scope and utility of exo-mechanism proximity-accelerated reactions in engineered receptor-ligand systems are reported. We synthesized a series of electrophilic cyclosporin (CsA) derivatives by varying electrophiles and linker le
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f8353064105d494310eb10885263e04
https://doi.org/10.1002/cbic.200400383
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8
Solid-Phase Synthesis of Dihydrovirginiamycin S1, a Streptogramin B Antibiotic
Autor: Peter J. Belshaw, Alex Shaginian, Marissa C. Rosen, Brock F. Binkowski
Publikováno v: Chemistry - A European Journal. 10:4334-4340
We describe the first solid-phase synthesis of dihydrovirginiamycin S(1), a member of the streptogramin B family of antibiotics, which are nonribosomal-peptide natural products produced by Streptomyces. These compounds, along with the synergistic gro
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3704b779593e8378927a861eaaa9f530
https://doi.org/10.1002/chem.200400267
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9
Aminoacyl-CoAs as Probes of Condensation Domain Selectivity in Nonribosomal Peptide Synthesis
Autor: Torsten Stachelhaus, Peter J. Belshaw, Christopher T. Walsh
Publikováno v: Science. 284:486-489
In nonribosomal biosynthesis of peptide antibiotics by multimodular synthetases, amino acid monomers are activated by the adenylation domains of the synthetase and loaded onto the adjacent carrier protein domains as thioesters, then the formation of
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5e7b3b89bc13f3d0e2f998a4830ef63
https://doi.org/10.1126/science.284.5413.486
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