DDK: The Outsourced Kinase of Chromosome Maintenance

Autor: Peter J. Gillespie, J. Julian Blow

Publikováno v: Biology, Vol 11, Iss 6, p 877 (2022)

The maintenance of genomic stability during the mitotic cell-cycle not only demands that the DNA is duplicated and repaired with high fidelity, but that following DNA replication the chromatin composition is perpetuated and that the duplicated chroma

Externí odkaz: https://doaj.org/article/c62bac1a52f649a68578a224ed8c062b

Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1

Autor: Robert C. Alver, Gaganmeet Singh Chadha, Peter J. Gillespie, J. Julian Blow

Publikováno v: Cell Reports, Vol 18, Iss 10, Pp 2508-2520 (2017)

Dbf4-dependent kinases (DDKs) are required for the initiation of DNA replication, their essential targets being the MCM2-7 proteins. We show that, in Xenopus laevis egg extracts and human cells, hyper-phosphorylation of DNA-bound Mcm4, but not phosph

Externí odkaz: https://doaj.org/article/490b1fc46b504a26a468772351efb399

Xenopus Cdc7 executes its essential function early in S phase and is counteracted by checkpoint-regulated protein phosphatase 1

Autor: Wei Theng Poh, Gaganmeet Singh Chadha, Peter J. Gillespie, Philipp Kaldis, J. Julian Blow

Publikováno v: Open Biology, Vol 4, Iss 1 (2014)

The initiation of DNA replication requires two protein kinases: cyclin-dependent kinase (Cdk) and Cdc7. Although S phase Cdk activity has been intensively studied, relatively little is known about how Cdc7 regulates progression through S phase. We ha

Externí odkaz: https://doaj.org/article/b4b6775fab8c489199639452ff5020a1

Histone H4K20 methylation mediated chromatin compaction threshold ensures genome integrity by limiting DNA replication licensing

Autor: Eric Julien, Fanny Izard, Klaus Hansen, Jens Vilstrup Johansen, Mads Lerdrup, David Llères, Birthe Fahrenkrog, Muhammad Shoaib, David Walter, Claus Storgaard Sørensen, J. Julian Blow, Peter J. Gillespie

Publikováno v: Nature communications, 9 (1
Shoaib, M, Walter, D, Gillespie, P J, Izard, F, Fahrenkrog, B, Lleres, D, Lerdrup, M, Johansen, J V, Hansen, K, Julien, E, Blow, J J & Sørensen, C S 2018, ' Histone H4K20 methylation mediated chromatin compaction threshold ensures genome integrity by limiting DNA replication licensing ', Nature Communications, vol. 9, no. 1, 3704, pp. 1-11 . https://doi.org/10.1038/s41467-018-06066-8
Nature Communications
Nature Communications, Nature Publishing Group, 2018, 9, pp.3704. ⟨10.1038/s41467-018-06066-8⟩
Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
Nature Communications, 2018, 9, pp.3704. ⟨10.1038/s41467-018-06066-8⟩

The decompaction and re-establishment of chromatin organization immediately after mitosis is essential for genome regulation. Mechanisms underlying chromatin structure control in daughter cells are not fully understood. Here we show that a chromatin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67f5e427615bf9141aad98cd7784b0cb
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/282441

The High-Affinity Interaction between ORC and DNA that Is Required for Replication Licensing Is Inhibited by 2-Arylquinolin-4-Amines

Autor: Nicola J, Gardner, Peter J, Gillespie, Jamie T, Carrington, Emma J, Shanks, Stuart P, McElroy, Emma J, Haagensen, Julie A, Frearson, Andrew, Woodland, J Julian, Blow

Publikováno v: Cell Chemical Biology

Summary In late mitosis and G1, origins of DNA replication must be “licensed” for use in the upcoming S phase by being encircled by double hexamers of the minichromosome maintenance proteins MCM2–7. A “licensing checkpoint” delays cells in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5b0edbfede0ee5a9ecd527fca79ee891
https://pubmed.ncbi.nlm.nih.gov/28781123