Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Peter Haeberli"'
Publikováno v:
Bioconjugate Chemistry. 13:1071-1078
To evaluate potential improvement in tissue specific targeting and cellular uptake of therapeutic ribozymes, we have developed three new phosphoramidite reagents. These reagents can be used in automated solid-phase synthesis to produce oligonucleotid
Functionalized nucleoside 5′-triphosphates for in vitro selection of new catalytic ribonucleic acids
Autor:
Andrew T. Daniher, David Sweedler, Alexander Karpeisky, Peter Haeberli, Jasenka Matulic-Adamic, Leonid Beigelman
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 10:1299-1302
A series of novel 2'-modified nucleoside 5'-triphosphates was synthesized. The amino, imidazole, and carboxylate functionalities were attached to the 5-position of pyrimidine base of these molecules through alkynyl and alkyl spacers, respectively. Tw
Publikováno v:
Tetrahedron. 56:1047-1056
We developed several improved approaches toward 2′- O -methyl adenosine and guanosine and their N -acyl derivatives. (a) Transglycosylation of N 4 -acetyl-5′, 3′-di- O -acetyl-2′- O -methyl cytidine with N 6 -Bz-adenine provided N 6 -benzoyl-
Autor:
Victor Mokler, Lara Maloney, Francine E. Wincott, Nassim Usman, Peter Haeberli, Anthony DiRenzo, Leonid Beigelman, Jasenka Matulic-Adamic
Publikováno v:
Nucleosides and Nucleotides. 16:1933-1950
Novel 5′-amino-5′-deoxy-2′-O-methyl uridine, guanosine and adenosine 3′-O-phosphoramidites 5, 11, and 20, as well as protected 5′-mercapto-5′-deoxy-2′-O-methyl uridine 3′-O-phosphoramidite 23 were synthesized from 2′-O-methyl nucleo
Autor:
Arbi Khodadadi, Peter Haeberli, Leonid Beigelman, Qiaolian Liu, Anna Marie Pyle, Justin B. Green
Publikováno v:
Journal of Molecular Biology. 267:163-171
The 2′-hydroxyl on a specific bulged adenosine is the nucleophile during the first step of splicing by group II introns. To understand the means by which the ribozyme core recognizes this adenosine, it was mutagenized and effects on catalytic activ
Autor:
Nassim Usman, Lara Maloney, Peter Haeberli, Anthony DiRenzo, Francine E. Wincott, Susan Grimm, Kurt Levy
Publikováno v:
Nucleosides and Nucleotides. 15:1687-1700
A systematic study of the catalytic activity and nuclease stability of selectively modified hammerhead ribozymes has resulted in the identification of a generic motif containing 5 ribose residues and 31 2′- modified sugars (1). This substructure ha
Autor:
Leonid Beigelman, Alexander Karpeisky, James McSwiggen, Varykina G. Thackray, Kristi Jensen, Francine E. Wincott, Nassim Usman, Jasenka Matulic-Adamic, Kenneth G. Draper, Danuta Tracz, David Sweedler, Anil S. Modak, Carolyn Gonzalez, Susan Grimm, Peter Haeberli, Anthony DiRenzo
Publikováno v:
Journal of Biological Chemistry. 270:25702-25708
A systematic study of selectively modified, 36-mer hammerhead ribozymes has resulted in the identification of a generic, catalytically active and nuclease stable ribozyme motif containing 5 ribose residues, 29-30 2'-O-Me nucleotides, 1-2 other 2'-mod
Publikováno v:
The Journal of Organic Chemistry. 60:2563-2569
Autor:
Peter Haeberli, Keith Jarvis, Alexander Karpeisky, David Sweedler, Leonid Beigelman, Javier Read
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 12:3345-3347
2'-Deoxy-2'-N-phthaloyl nucleosides were prepared from arabino nucleosides by triflate displacement with phthalimide in the presence of DBU. The corresponding phosphoramidites suitable for automated oligonucleotide synthesis were also synthesized. Th
Autor:
Alexander Karpeisky, Andrew T. Daniher, Jasenka Matulic-Adamic, Peter Haeberli, Leonid Beigelman, David Sweedler
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids. 20:1113-1115
2′-Modified pyrimidine nucleoside 5′-triphosphates comprising amino, imidazole and carboxylate functionality attached to the 5-position of the base were synthesized. Two different phosphorylation methods were used to optimize the yields of these