Zobrazeno 1 - 10
of 75
pro vyhledávání: '"Peter Gee"'
Autor:
Peter Gee, Mandy S. Y. Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F. Yang, Mio Iwasaki, Xiou H. Wang, Matthew A. Waller, Nanako Shirai, Yasuko O. Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita, Kumiko A. Iwabuchi, Masahiko Yasuda, Huaigeng Xu, Takeshi Noda, Jun Komano, Hidetoshi Sakurai, Naoto Inukai, Akitsu Hotta
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Expression of Cas9 and gRNA from viral vectors in vivo may cause off-target activity. Here the authors present NanoMEDIC, which uses nanovesicles to transiently deliver editing machinery to hard-to-transfect cells.
Externí odkaz:
https://doaj.org/article/4d0fe2ef9a604f0fb6865d1e08b1ed50
Publikováno v:
STAR Protocols, Vol 2, Iss 4, Pp 100965- (2021)
Summary: Selection-free, scarless genome editing in human pluripotent stem cells (PSCs) by utilizing ribonucleoprotein (RNP) of CRISPR-Cas9 is a useful tool for a variety of applications. However, the process can be hampered by time-consuming subclon
Externí odkaz:
https://doaj.org/article/450590eb0003428ebdcbf99ca83667f2
Autor:
Hiroyuki Morisaka, Kazuto Yoshimi, Yuya Okuzaki, Peter Gee, Yayoi Kunihiro, Ekasit Sonpho, Huaigeng Xu, Noriko Sasakawa, Yuki Naito, Shinichiro Nakada, Takashi Yamamoto, Shigetoshi Sano, Akitsu Hotta, Junji Takeda, Tomoji Mashimo
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Class 1 CRISPR systems are not as developed for genome editing as Class 2 systems are. Here the authors show that Cas3 can be used to generate functional knockouts and knock-ins, as well as Cas3-mediated exon-skipping in DMD cells.
Externí odkaz:
https://doaj.org/article/dce5c9fe636b4d0abf512a35936827ae
Publikováno v:
International Journal of Population Data Science, Vol 3, Iss 4 (2018)
Introduction While public engagement activity in healthcare may include a focus on uses of patient data, use of social care/ education data may be relatively overlooked. The public may be less aware about how their social care data may be used. Some
Externí odkaz:
https://doaj.org/article/2793c99bc76e4cd88448d8a91208dbaf
Publikováno v:
Stem Cells International, Vol 2017 (2017)
In the past decade, the development of two innovative technologies, namely, induced pluripotent stem cells (iPSCs) and the CRISPR Cas9 system, has enabled researchers to model diseases derived from patient cells and precisely edit DNA sequences of in
Externí odkaz:
https://doaj.org/article/1d308f0739c643f6ac9383a10ccfabd9
Autor:
Joseph A Hollenbaugh, Peter Gee, Jonathon Baker, Michele B Daly, Sarah M Amie, Jessica Tate, Natsumi Kasai, Yuka Kanemura, Dong-Hyun Kim, Brian M Ward, Yoshio Koyanagi, Baek Kim
Publikováno v:
PLoS Pathogens, Vol 9, Iss 6, p e1003481 (2013)
SAMHD1 is a newly identified anti-HIV host factor that has a dNTP triphosphohydrolase activity and depletes intracellular dNTP pools in non-dividing myeloid cells. Since DNA viruses utilize cellular dNTPs, we investigated whether SAMHD1 limits the re
Externí odkaz:
https://doaj.org/article/a3545c06aea647e99748255eb628b99e
Autor:
Wilfred van Gunsteren, Thereza Soares, Lukas Schuler, Jed Pitera, Christine Peter, Chris Oostenbrink, Tomas Hansson, Alice Glättli, Peter Gee, Xavier Daura, Markus Christen, Indira Chandrasekhar, Roland Bürgi, Dirk Bakowies, Haibo Yu
Publikováno v:
CHIMIA, Vol 55, Iss 10 (2001)
The group for computer-aided chemistry at the ETH Zürich focuses its research on the development of methodology to simulate the behavior of biomolecular systems and the use of simulation techniques to analyze and understand biomolecular processes at
Externí odkaz:
https://doaj.org/article/5293de546cc54b67882d60d8bf81adc1
Autor:
Sayaka Deguchi, Kaori Kosugi, Rina Hashimoto, Ayaka Sakamoto, Masaki Yamamoto, Rafal P Krol, Peter Gee, Ryosuke Negoro, Takeshi Noda, Takuya Yamamoto, Yu-suke Torisawa, Miki Nagao, Kazuo Takayama
Publikováno v:
PNAS Nexus. 2(3)
SARS-CoV-2 induces severe organ damage not only in the lung but also in the liver, heart, kidney, and intestine. It is known that COVID-19 severity correlates with liver dysfunction, but few studies have investigated the liver pathophysiology in COVI
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da09ce14684c4422785551c74f3f127f
http://hdl.handle.net/2433/279615
http://hdl.handle.net/2433/279615
Publikováno v:
Methods in Molecular Biology ISBN: 9781071627716
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bce7440890f6b252c62a8a1489f616af
https://doi.org/10.1007/978-1-0716-2772-3_22
https://doi.org/10.1007/978-1-0716-2772-3_22
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2587
The CRISPR-Cas9 system has quickly become the standard tool for genome editing. To deliver this system to target cells, adeno-associated virus (AAV) vectors are commonly used. In fact, AAV vectors have been utilized to deliver the CRISPR-Cas9 system
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0cf2c9a1477e032d925876c0a41a42c6
https://pubmed.ncbi.nlm.nih.gov/36401042
https://pubmed.ncbi.nlm.nih.gov/36401042