Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Peter G. Condliffe"'
Autor:
Kenji Sorimach, Peter G. Condliffe
Publikováno v:
International Journal of Peptide and Protein Research. 12:1-6
In the present study SDS polyacrylamide gel electrophoresis was used to reinvestigate the conditions under which b-TSH dissociates into its constituent subunits. The effects of SDS, itself, urea, acid pH and temperature were assessed by the decrease
This volume contains the proceedings of a conference held at the National Institutes of Health in Bethesda on March 21-23. 1988. jointly sponsored by the International Council for Control of Iodine Deficiency Disorders (ICCIDD) and the Fogarty Intern
Autor:
Peter G. Condliffe, Kenji Sorimachi
Publikováno v:
Biochemical and Biophysical Research Communications. 77:526-532
Sodium dodecylsulfate (SDS) can be removed from protein by gel electrophoresis. This principle is useful for separating protein bands which are close to each other in SDS gel electrophoresis. We accomplished this by “two-stage” gel electrophoresi
Publikováno v:
Journal of Biological Chemistry. 241:5205-5212
1. The effect of acidification on the fluorescence, absorption, viscosity, sedimentation, optical rotatory dispersion, and polarization of fluorescence of bovine growth hormone has been measured. All the data are consistent with a molecular transitio
Publikováno v:
Endocrinology. 76:27-34
Extracts of human pituitary glands containing FSH, LH and TSH were fractionated by chromatography on CM-C. FSH was not adsorbed on CM-C at pH 5.85 in 0.005M citrate-phosphate buffer and was thus separated from LH and TSH, which are strongly bound to
Autor:
Peter G. Condliffe, Robert W. Bates
Publikováno v:
Journal of Biological Chemistry. 223:843-852
Autor:
Peter G. Condliffe
Publikováno v:
Endocrinology. 72:893-896
Human thyroid-stimulating hormone (H-TSH) has been prepared from a side fraction, obtained during the isolation of human growth hormone, which contained thyroid-stimulating and gonadotrophic activity. After a preliminary equilibration step on Sephade
Publikováno v:
Journal of Biological Chemistry. 218:41-52
Publikováno v:
Journal of Biological Chemistry. 209:133-143
Publikováno v:
Endocrinology. 78:98-102
When bovine growth hormone was acidified from pH 5 to 2.5 the fluorescence of the tryptophan residues increased about 6-fold. This exaltation appears to be specific for bovine and ovine growth hormones. This property of bovine growth hormone can be u