Zobrazeno 1 - 10
of 87
pro vyhledávání: '"Peter G Gillespie"'
Autor:
Kateri J Spinelli, Peter G Gillespie
Publikováno v:
PLoS ONE, Vol 7, Iss 12, p e51874 (2012)
We optimized Fluo-4 AM loading of chicken cochlea to report hair-bundle Ca(2+) signals in populations of hair cells. The bundle Ca(2+) signal reported the physiological state of the bundle and cell; extruding cells had very high bundle Fluo-4 fluores
Externí odkaz:
https://doaj.org/article/09c1d267d19d46aba81e003e0d37ec71
Publikováno v:
PLoS ONE, Vol 7, Iss 10, p e46765 (2012)
Transient transfection of hair cells has proven challenging. Here we describe modifications to the Bio-Rad Helios Gene Gun that, along with an optimized protocol, improve transfection of bullfrog, chick, and mouse hair cells. The increased penetratin
Externí odkaz:
https://doaj.org/article/c0648dc13a3148fa94e8052613fec8f0
Autor:
Yue Yang, Min Dai, Teresa M Wilson, Irina Omelchenko, John E Klimek, Phillip A Wilmarth, Larry L David, Alfred L Nuttall, Peter G Gillespie, Xiaorui Shi
Publikováno v:
PLoS ONE, Vol 6, Iss 1, p e16547 (2011)
BACKGROUND:The endothelial-blood/tissue barrier is critical for maintaining tissue homeostasis. The ear harbors a unique endothelial-blood/tissue barrier which we term "blood-labyrinth-barrier". This barrier is critical for maintaining inner ear home
Externí odkaz:
https://doaj.org/article/658bb5294e934dafb47e1724f35b83fd
Publikováno v:
The Journal of Neuroscience. 32:4600-4609
The plasma membrane of vertebrate hair bundles interacts intimately with the bundle cytoskeleton to support mechanotransduction and homeostasis. To determine the membrane composition of bundles, we used lipid mass spectrometry with purified chick ves
Autor:
Stephen Shaw, Leopoldo Santos-Argumedo, José L. Maravillas-Montero, Peter G. Gillespie, Genaro Patino-Lopez
Publikováno v:
The Journal of Immunology. 187:3053-3063
Myosin 1c (Myo1c) is a member of the unconventional class I myosins of vertebrates, which directly link the plasma membrane with the microfilament cortical web. Although this molecular motor has been implicated in cell functions such as cytoskeleton
Autor:
Atteeq U. Rehman, Saima Riazuddin, Matthias Hammerschmidt, Naseebullah Kakar, Kimia Kahrizi, Michael S. Hildebrand, Shaheen N. Khan, Jennifer Webster, Rehan S. Shaikh, Sulman Basit, Rana A. Ali, Gudrun Nürnberg, Guntram Borck, Hossein Najmabadi, Suzanne M. Leal, Ingrid Goebel, Kwanghyuk Lee, Peter G. Gillespie, Zubair M. Ahmed, Qamar Javed, Hans Martin Pogoda, Jamil Ahmad, A. Eliot Shearer, Muhammad Ansar, Nabilah Nasreen, William J. Kimberling, Robert J. Morell, Adeel Ahmad, Melanie Bahlo, Richard J.H. Smith, Nicolas Grillet, Simon von Ameln, Christian Kubisch, Martin R. Schiller, Bernd Wollnik, Wasim Ahmad, Dietrich A. Stephan, Thomas B. Friedman, Peter Nürnberg, Ulrich Müller, Sheikh Riazuddin, Nicole C. Meyer
Publikováno v:
The American Journal of Human Genetics. 88:127-137
By using homozygosity mapping in a consanguineous Pakistani family, we detected linkage of nonsyndromic hearing loss to a 7.6 Mb region on chromosome 3q13.31-q21.1 within the previously reported DFNB42 locus. Subsequent candidate gene sequencing iden
Autor:
Peter G. Gillespie, Kateri J. Spinelli, Rachel A. Dumont, Leona H. Gagnon, Katherine W. Saylor, James M. Pagana, Jung-Bum Shin, Phillip A. Wilmarth, Larry L. David, Kenneth R. Johnson, Chantal M. Longo-Guess
Publikováno v:
Journal of Neuroscience. 30:9683-9694
The quantitative trait locus ahl8 is a key contributor to the early-onset, age-related hearing loss of DBA/2J mice. A non-synonymous nucleotide substitution in the mouse fascin-2 gene (Fscn2) is responsible for this phenotype, confirmed by wild-type
Autor:
Ulrich Müller, Peter G. Gillespie
Publikováno v:
Cell. 139:33-44
Mechanotransduction, the transformation of mechanical force into an electrical signal, allows living organisms to hear, register movement and gravity, detect touch, and sense changes in cell volume and shape. Hair cells in the inner ear are specializ
Autor:
Rachel A. Dumont, Ulrich Müller, Peter G. Gillespie, Concepción Lillo, Edith Hintermann, Anna Sczaniecka, Takashi Sato, David S. Williams, Nicolas Grillet, Wei Xiong, Piotr Kazmierczak, Martin Schwander, Bechara Kachar, Anna Reynolds
Publikováno v:
Neuron. 62:375-387
SummaryIn hair cells, mechanotransduction channels are gated by tip links, the extracellular filaments that consist of cadherin 23 (CDH23) and protocadherin 15 (PCDH15) and connect the stereocilia of each hair cell. However, which molecules mediate c
Autor:
Yosuke Iizumi, Michinaga Ogawa, Chihiro Sasakawa, Hiroshi Sagara, Peter G. Gillespie, Yasuaki Kabe, Hiroshi Handa, Kanako Kume, Motoki Azuma, Takeshi Nagai
Publikováno v:
Cell Host & Microbe. 2(6):383-392
SummaryEnteropathogenic Escherichia coli (EPEC) destroys intestinal microvilli and suppresses phagocytosis by injecting effectors into infected cells through a type III secretion system (TTSS). EspB, a component of the TTSS, is also injected into the