Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Peter Dehm"'
Publikováno v:
European Journal of Biochemistry. 46:107-116
Antisera were prepared by immunizing a rabbit with procollagen synthesized and secreted by cells from chick embryo tendons. Specific antibodies were then purified from the antisera by using an immunoadsorbent which contained the isolated NHz-terminal
Autor:
Paul Bornstein, Peter Dehm, Bjorn R. Olsen, Elizabeth D. Hay, Russell Ross, K von der Mark, C Nist
Publikováno v:
The Journal of Cell Biology
Three distinct antiprocollagen preparations were characterized and used in immunocytochemical staining of chick embryo corneal and tendon cells. The several ferritin-conjugated antibody preparations permitted similar location of procollagen in the ci
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 236:517-527
Previous studies demonstrated that the proline analogue azetidine-2-carboxylic acid was incorporated in collagen synthesized in vitro and that the collagen containing the analogue was extruded from cells at a decreased rate. In the present study azet
Publikováno v:
FEBS Letters. 17:245-248
Autor:
Peter Dehm, Darwin J. Prockop
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 264:375-382
Matrix-free cells were isolated from chick embryo tendons and incubated with [14Cproline in order to follow the time course for the synthesis and secretion of [14C]-collagen. The results indicated a lag of 2–3 min between the time at which the inco
Autor:
Peter Dehm, Darwin J. Prockop
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 240:358-369
Matrix-free cells were isolated from chick embryo tendons by digesting the tendons with trypsin and bacterial collagenase. Microscopic examination of the isolated cells indicated that they were essentially homogeneous and testing the cells with the T
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 278:601-605
Matrix-free cells from the tendons of chick embryos were incubated with cis-4-hydroxy-L-proline, a proline analogue previously shown to be incorporated into collagen and other proteins. The analogue (200 μg/ml) decreased the secretion of 14C-labeled
Publikováno v:
Journal of Biological Chemistry. 248:720-729
When matrix-free tendon cells from chick embryos were incubated with [14C]proline and then extracted with sodium dodecyl sulfate and mercaptoethanol, a major part of the newly synthesized 14C-protein was found to consist of collagen polypeptides whic
Autor:
Arnold Nordwig, Peter Dehm
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 160:293-295
Publikováno v:
Collagen and related research. 2(1)
The acid-insoluble fraction of murine tumor basement membrane produced by the EHS sarcoma was extracted following reduction of disulfide bonds under nondenaturing conditions. Extracts prepared from tumors grown in either lathyritic or normal animals