Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Peter D.G. Dean"'
Publikováno v:
Analytical Biochemistry. 122:186-193
Pregnancy-specific β 1 2 -glycoprotein was purified to 99% purity by sequential chromatography on carboxymethyl-cellulose (to remove other pregnancy-specific β 1 -glycoprotein variants), Cibacron blue 3G-A-Matrex Gel, Procion turquoise MX-G-Matrex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::297bb404cf8209d11fb7ad32c7834bba
https://doi.org/10.1016/0003-2697(82)90269-x
https://doi.org/10.1016/0003-2697(82)90269-x
Publikováno v:
FEBS Letters. 83:256-259
1. Introduction Since the original purification of ferritin by Laufberger [l] using cadmium salt precipitation a number of purification techniques have been described using various combinations of heat denaturation and differential precipitation with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4bd5f33fc623fc47f507150e1c6288b
https://doi.org/10.1016/0014-5793(77)81017-x
https://doi.org/10.1016/0014-5793(77)81017-x
Publikováno v:
European Journal of Biochemistry. 41:353-357
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c271adfa3324052f23836f7cd5a284b
https://doi.org/10.1111/j.1432-1033.1974.tb03277.x
https://doi.org/10.1111/j.1432-1033.1974.tb03277.x
Publikováno v:
European Journal of Biochemistry. 55:201-209
The effect of pH and temperature on the capacity and binding of Bacillus stearothermophilus, alcohol dehydrogenase and phosphofructokinase to N6-(6-aminohexyl)-5′-AMP-Sepharose has been examined. Specific elution from the substituted AMP-Sepharose
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a90c3be7be61491347e6eb5956b1ae4e
https://doi.org/10.1111/j.1432-1033.1975.tb02152.x
https://doi.org/10.1111/j.1432-1033.1975.tb02152.x
Autor:
Peter D.G. Dean
Publikováno v:
TrAC Trends in Analytical Chemistry. 2:80-83
In recent years affinity chromatography has rapidly developed as a method of protein separation. The growing interest in enzymes as tools for genetic engineering has prompted investigations into novel methods for their isolation, with the result that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9d3e8f5c326a804010f4e877dc71abde
https://doi.org/10.1016/0165-9936(83)85023-7
https://doi.org/10.1016/0165-9936(83)85023-7
Publikováno v:
Journal of Chromatography A. 235:501-506
The combination of affinity chromatography and immunoassay provides a novel method for the quantitation of steroids in biological fluids. Anti-digoxin and anti-oestriol-16α-glucuronide antibodies were immobilised to cellulose strips, and used for as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::457f2d80da7db8226e14e3c379545fed
https://doi.org/10.1016/s0021-9673(00)85914-2
https://doi.org/10.1016/s0021-9673(00)85914-2
Publikováno v:
Biological Mass Spectrometry. 10:512-527
On the probe tip of a fast atom bombardment mass spectrometer, boronic acids react with a wide range of trifunctional compounds of appropriate configuration yielding negatively-charged boronate cage compounds. Formation of 5- to 8-membered rings occu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ab7de961e523c82b495bb46780504ff7
https://doi.org/10.1002/bms.1200100907
https://doi.org/10.1002/bms.1200100907
Publikováno v:
Journal of Chromatography A. 210:267-278
Several different aminophenylboronic acid (APBA)-agarose matrices have been compared. Nucleotides are able to bind to these matrices in the presence of either NaCl or MgCl2. NAD+ and FAD are more tightly bound to these columns when compared with othe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d3476e49572b169cbaa3a039b2fb786b
https://doi.org/10.1016/s0021-9673(00)97837-3
https://doi.org/10.1016/s0021-9673(00)97837-3
Publikováno v:
Biochemical Journal. 209:771-779
Haemoglobins from diabetic and non-diabetic individuals have been separated by affinity chromatography using immobilized phenylboronate, which interacts specifically with diol-containing compounds such as glycosylated haemoglobin. The effects of liga
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de0bb4ccd3ef9fdfd3ce56ef33b84948
https://doi.org/10.1042/bj2090771
https://doi.org/10.1042/bj2090771
Publikováno v:
Analytical Biochemistry. 105:1-5
Some of the important controlling factors involved in the electrophoretic desorption of material from affinity matrices (the electrophoresis current, the matrix thickness, and the electrophoresis buffer molarity and temperature) have been investigate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05d781856261ec8a927b51c3196096e3
https://doi.org/10.1016/0003-2697(80)90413-3
https://doi.org/10.1016/0003-2697(80)90413-3