Výsledky vyhledávání - "Peter C, Simons"

Longitudinal Assessment of Cytokine Expression and Plasminogen Activation in Hantavirus Cardiopulmonary Syndrome Reveals Immune Regulatory Dysfunction in End-Stage Disease

Autor: Virginie Bondu, Susan L Tigert, Tione Buranda, Laura V. Gonzalez Bosc, Xuexian O Yang, Steven B. Bradfute, Michelle Harkins, Daniel A. Lawrence, Yan Guo, Samuel M Goodfellow, Cana Tompkins, Devon Chabot-Richards, Peter C. Simons

Publikováno v: Viruses, Vol 13, Iss 1597, p 1597 (2021)
Viruses
Volume 13
Issue 8

Pathogenic New World orthohantaviruses cause hantavirus cardiopulmonary syndrome (HCPS), a severe immunopathogenic disease in humans manifested by pulmonary edema and respiratory distress, with case fatality rates approaching 40%. High levels of infl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ba69f6438d69418d50e91b986f176e3
https://www.mdpi.com/1999-4915/13/8/1597

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Integrin activation is an essential component of SARS-CoV-2 infection

Autor: Diane S. Lidke, Peter C. Simons, Tione Buranda, Steven B. Bradfute, Derek A. Rinaldi, Alison M. Kell, Virginie Bondu

Publikováno v: bioRxiv
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)

Cellular entry of coronaviruses depends on binding of the viral spike (S) protein to a specific cellular receptor, the angiotensin-converting enzyme 2 (ACE2). Furthermore, the viral spike protein expresses an RGD motif, suggesting that cell surface i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::195cc4e21a554892be93f67decdc66d1
https://pubmed.ncbi.nlm.nih.gov/34312625

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Low-affinity binding incisto P2Y2R mediates force-dependent integrin activation during hantavirus infection

Autor: Tione Buranda, Chenyu Wu, Laurie Erb, Wenpeng Cao, Jieqing Zhu, X. Frank Zhang, Virginie Bondu, Peter C. Simons, Jennifer M. Gillette

Publikováno v: Molecular Biology of the Cell. 28:2887-2903

Pathogenic hantaviruses bind to the plexin-semaphorin-integrin (PSI) domain of inactive, β3integrins. Previous studies have implicated a cognate cis interaction between the bent conformation β5/β3integrins and an arginine-glycine-aspartic acid (RG

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ed25f729c58d28e4cf9c3b78a23e6cea
https://doi.org/10.1091/mbc.e17-01-0082

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Small-Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases

Autor: Angela Wandinger-Ness, Virginie Bondu, Tione Buranda, Peter C. Simons

Publikováno v: Methods in Molecular Biology ISBN: 9781493986118

Small, monomeric guanine triphosphate hydrolases (GTPases) are ubiquitous cellular integrators of signaling. A signal activates the GTPase, which then binds to an effector molecule to relay a signal inside the cell. The GTPase effector trap flow cyto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a3e3d3919d32f951f4c99fc8b30999d8
https://doi.org/10.1007/978-1-4939-8612-5_13

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Low-affinity binding in

Autor: Virginie, Bondu, Chenyu, Wu, Wenpeng, Cao, Peter C, Simons, Jennifer, Gillette, Jieqing, Zhu, Laurie, Erb, X Frank, Zhang, Tione, Buranda

Publikováno v: Molecular Biology of the Cell

Atomic force microscopy is used to establish that low-affinity integrins bind in cis to P2Y2R. Integrin activation is initiated by a membrane-normal switchblade motion triggered by integrin priming after the virus binds to the integrin PSI domain. Te

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a370a8118ebcbf0d472a58926414a252
https://pubmed.ncbi.nlm.nih.gov/28835374

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A High-Throughput Flow Cytometry Assay for Identification of Inhibitors of 3',5'-Cyclic Adenosine Monophosphate Efflux

Autor: Alexandre Chigaev, Peter C. Simons, Yelena Smagley, Dominique Perez, Larry A. Sklar

Publikováno v: Methods in Molecular Biology ISBN: 9781493936717

Assays to identify small molecule inhibitors of cell transporters have long been used to develop potential therapies for reversing drug resistance in cancer cells. In flow cytometry, these approaches rely on the use of fluorescent substrates of trans

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::172f6ba8cc68479b10756cedb00fd152
https://pubmed.ncbi.nlm.nih.gov/27316999

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High-Throughput Screen for the Chemical Inhibitors of Antiapoptotic Bcl-2 Family Proteins by Multiplex Flow Cytometry

Autor: Cristian Bologa, Dayong Zhai, Arnold C. Satterthwait, John C. Reed, Tudor I. Oprea, Mark B. Carter, Susan M. Young, Peter C. Simons, Bruce S. Edwards, Larry A. Sklar, Ramona Curpan

Publikováno v: ASSAY and Drug Development Technologies. 9:465-474

The human Bcl-2 family includes six antiapoptotic members (Bcl-2, Bcl-B, Bcl-W, Bcl-X(L), Bfl-1, and Mcl-1) and many proapoptotic members, wherein a balance between the two determines cell life or death in many physiological and disease contexts. Ele

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c21a4818ee84cc98db58fa1412b20e5
https://doi.org/10.1089/adt.2010.0363

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Simultaneous in vitro molecular screening of protein-peptide interactions by flow cytometry, using six Bcl-2 family proteins as examples

Autor: Peter C. Simons, John C. Reed, Dayong Zhai, Bruce S. Edwards, Anna Waller, Mark B. Carter, Susan M. Young, Larry A. Sklar

Publikováno v: Nature Protocols. 6:943-952

The Bcl-2 family contains six anti-apoptotic members, each with a hydrophobic pocket where BH3 helixes (Bcl-2 homology region 3) bind. This binding quenches apoptotic signals from activated BH3 family members. Many tumor cells either have increased e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d7262911c02751da888aed4354cbf46
https://doi.org/10.1038/nprot.2011.339

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Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics by guanine nucleotides

Autor: Larry A. Sklar, Tione Buranda, Eric R. Prossnitz, James C. Garrison, Gabriel P. Lopez, Peter C. Simons, William E. McIntire, Yang Wu

Publikováno v: Analytical Biochemistry. 371:10-20

We have used rapid mix flow cytometry to analyze the early subsecond dynamics of the disassembly of ternary complexes of G-protein coupled receptors (GPCRs) immobilized on beads to examine individual steps associated with guanine nucleotide activatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cacfa1ac8ba4119344c1b9776471d28c
https://doi.org/10.1016/j.ab.2007.08.011

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A Pan-GTPase Inhibitor as a Molecular Probe

Autor: Kristine Gouveia, Jeffrey Aubé, Lin Hong, Jacob O. Agola, Chad E. Schroeder, Jennifer E. Golden, Peter C. Simons, Elsa Romero, Yuna Guo, Soumik BasuRay, Matthew Garcia, Anna Waller, Angela Wandinger-Ness, Oleg Ursu, Denise S. Simpson, Larry A. Sklar, Mark B. Carter

Publikováno v: PLoS ONE
PLoS ONE, Vol 10, Iss 8, p e0134317 (2015)

Overactive GTPases have often been linked to human diseases. The available inhibitors are limited and have not progressed far in clinical trials. We report here a first-in-class small molecule pan-GTPase inhibitor discovered from a high throughput sc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bba672e94ba3f34355e98d6f5fdb0bb4
http://europepmc.org/articles/PMC4527730

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