Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Peter Bults"'
Autor:
Oladapo Olaleye, Baubek Spanov, Peter Bults, Anna van der Voort, Natalia Govorukhina, Gabe S. Sonke, Peter Horvatovich, Nico C. van de Merbel, Rainer Bischoff
Publikováno v:
Drug Metabolism and Disposition, 50(12), 249-256. AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
Therapeutic proteins (TPs) are known to be heterogeneous due to modifications that occur during the production process and storage. Modifications may also occur in TPs after their administration to patients due to in vivo biotransformation. Ligand bi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae87696c75e933ab48fb7eec5070321d
https://research.rug.nl/en/publications/de84de11-0a42-40fd-b01c-be0b04d60f7e
https://research.rug.nl/en/publications/de84de11-0a42-40fd-b01c-be0b04d60f7e
Autor:
Peter Bults
Unlike the current situation for small-molecule drugs, the biotransformation of biopharmaceuticals is a largely unexplored field. Although much attention is paid to (the prevention of) degradation and structural alteration of protein-based drugs in p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3cd229a6550ec990967f6812ed2753f
https://doi.org/10.33612/diss.206613225
https://doi.org/10.33612/diss.206613225
Publikováno v:
Journal of Chromatography A, 1603, 417-421. ELSEVIER SCIENCE BV
Deamidation of asparagine (Asn) residues of monoclonal antibodies (mAbs) plays a pivotal role in the in vivo/vitro degradation or efficacy loss of biopharmaceuticals. However, a major challenge for MS analysis of deamidation of Asn-containing peptide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc2182b58754db3ed15192fe9d4018ac
https://doi.org/10.1016/j.chroma.2019.05.053
https://doi.org/10.1016/j.chroma.2019.05.053
Autor:
Frank Klont, Simon D. Pouwels, Peter Horvatovich, Nick H. T. ten Hacken, Peter Bults, Rainer Bischoff, Nico C van de Merbel
Publikováno v:
Talanta, 202, 507-513. ELSEVIER SCIENCE BV
Quantification of intact proteins in complex biological matrices by liquid chromatography-mass spectrometry (LC-MS) is a promising analytical strategy but is technically challenging, notably for concentrations at or below the ng/mL level. Therefore,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f354dbb6ee43669d48612cf78f1d1a4e
https://research.rug.nl/en/publications/677ac90e-ab4d-442e-897b-49b95ebe2d32
https://research.rug.nl/en/publications/677ac90e-ab4d-442e-897b-49b95ebe2d32
Publikováno v:
Journal of Chromatography B
This review discusses the challenges of quantitative protein bioanalysis by LC-MS at the protein level. We will notably address the possibilities and current limitations of protein sample preparation, separation by LC, the challenge of interpreting p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c415474d98b728d93a646cb1c3b6f3e1
https://doi.org/10.1016/j.jchromb.2019.01.032
https://doi.org/10.1016/j.jchromb.2019.01.032
Analytical and pharmacological consequences of the in vivo deamidation of trastuzumab and pertuzumab
Autor:
Peter Bults, Anna van der Voort, Coby Meijer, Gabe S. Sonke, Rainer Bischoff, Nico C. van de Merbel
Publikováno v:
Analytical and Bioanalytical Chemistry, 414(4). SPRINGER HEIDELBERG
A liquid chromatography-tandem mass spectrometry method is presented for the quantitative determination of the in vivo deamidation of the biopharmaceutical proteins trastuzumab and pertuzumab at an asparagine in their complementarity determining regi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8904eed10677e500106a6d246674acf
https://doi.org/10.1007/s00216-021-03756-z
https://doi.org/10.1007/s00216-021-03756-z
Publikováno v:
Analytical Chemistry, 88(3), 1871-1877. AMER CHEMICAL SOC INC
An LC-MS/MS-based method is described for quantitatively monitoring the in vivo deamidation of the biopharmaceutical monoclonal antibody trastuzumab at a crucial position in its complementarity determining region (CDR). The multiplexed LC-MS/MS assay
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e817f81b5f03bafa3b8b5f1ee88f79ea
https://doi.org/10.1021/acs.analchem.5b04276
https://doi.org/10.1021/acs.analchem.5b04276
Publikováno v:
Journal of Chromatography B, 1144:122079
The quantitative determination of intact proteins in biological samples by LC with high-resolution MS detection can be a useful alternative to ligand-binding assays or LC-MS-based quantification of a surrogate peptide after protein digestion. The 22-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01e61cfc6cc8107a5e1e0f20bee4a10d
https://doi.org/10.1016/j.jchromb.2020.122079
https://doi.org/10.1016/j.jchromb.2020.122079
Autor:
Anders Sonesson, Peter Bults, Nico C van de Merbel, Marcel Meints, Rainer Bischoff, Magnus Knutsson
Publikováno v:
Bioanalysis, 10(13), 1009-1021. FUTURE SCI LTD
Aim: Protein quantitation by digestion of a biological sample followed by LC–MS analysis of a signature peptide can be a challenge because of the high complexity of the digested matrix. Results/methodology: The use of LC with high-resolution (quadr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a3425f0fe3951f9e89e77a233d218c6
https://pubmed.ncbi.nlm.nih.gov/29952231
https://pubmed.ncbi.nlm.nih.gov/29952231
Publikováno v:
Expert review of proteomics, 12(4), 355-374. Taylor & Francis Ltd
The quantification of proteins (biopharmaceuticals or biomarkers) in complex biological samples such as blood plasma requires exquisite sensitivity and selectivity, as all biological matrices contain myriads of proteins that are all made of the same
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cce2861668d1818f7bc647d68439ecb5
https://doi.org/10.1586/14789450.2015.1050384
https://doi.org/10.1586/14789450.2015.1050384