Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Peter Bellemann"'
Publikováno v:
Plant disease. 82(2)
Erwinia amylovora strains formed yellow colonies on minimal agar medium MM2 containing asparagine and copper sulfate (MM2Cu), in contrast to a white morphology on minimal agar without copper salt. Additionally, the colonies were mucoid to various ext
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bfa35c8a4713172be1b3fd6d43a1643
https://pubmed.ncbi.nlm.nih.gov/30856793
https://pubmed.ncbi.nlm.nih.gov/30856793
Publikováno v:
Acta Horticulturae
Monitoring of fireblight can be done by plating on semi-selective agar, by immunofluorescence or by hybridization of bacterial colonies with DNA from a 29 kb plasmid common to Erwinia amylovora. The specific but labour intensive procedure of DNA hybr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78b34006d5a5a31f5a397268e700d74d
https://doi.org/10.17660/actahortic.1993.338.6
https://doi.org/10.17660/actahortic.1993.338.6
Autor:
Dietlinde Schullerus, Klaus Geider, Peter Bellemann, Manfred Nimtz, David L. Coplin, Frank Bernhard
Publikováno v:
Microbiology (Reading, England). 142
DNA fragments with ams genes of Erwinia amylovora and cps genes of Erwinia stewartii were transferred to exopolysaccharide (EPS)-deficient mutants of the other species. The resulting EPSs were characterized by sensitivity to EPS-dependent bacteriopha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::815b6cfb00d677024215b789410ac575
https://pubmed.ncbi.nlm.nih.gov/8704950
https://pubmed.ncbi.nlm.nih.gov/8704950
Publikováno v:
Microbiological Research
The synthetic plasmid pfdC1 with the replication origin of phage fd and fd gene 2 grows autonomously in E. coli cells. DNA sequencing revealed several mutations compared to the fd genome causing reduced expression of viral gene 2 protein, which can b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf3611cf7c681bdf67de5055c4c3d9dc
https://pubmed.ncbi.nlm.nih.gov/8564362
https://pubmed.ncbi.nlm.nih.gov/8564362
Visualization of capsule formation by Erwinia amylovora and assays to determine amylovoran synthesis
Publikováno v:
International Journal of Biological Macromolecules
Exopolysaccharide (EPS) synthesis by Erwinia amylovora depends on environmental and genetic predispositions. To measure the amount of the acidic EPS amylovoran synthesized by E. amylovora cell cultures, a turbidity assay using cetylpyridinium salt wa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61e7f5484192372c89bbeb047e0d8953
https://www.ncbi.nlm.nih.gov/pubmed/7537077
https://www.ncbi.nlm.nih.gov/pubmed/7537077
Publikováno v:
Journal of Bacteriology
Galactose metabolism mutants of Erwinia amylovora were created by transposon insertions and characterized for their growth properties and interaction with plant tissue. The nucleotide sequence of the galE gene was determined. The gene, which encodes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb3addbe9ad32dbd9ec1cc383f7b61d4
https://www.ncbi.nlm.nih.gov/pubmed/7507102
https://www.ncbi.nlm.nih.gov/pubmed/7507102
Publikováno v:
Acta Horticulturae
A gene cluster for exopolysaccharide (EPS) synthesis of Erwinia amylovora was cloned in cosmid pEA109 and identified by complementation of Erwinia stewartii mutants deficient in EPS−production (cps). A corresponding cluster from E. stewartii (pES21
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72d5420d0457c3282a56b55e99ba2e82
http://www.actahort.org/books/338/338_41.htm
http://www.actahort.org/books/338/338_41.htm
Publikováno v:
Acta Horticulturae
The genetics of the two major exopolysaccharides of Erwinia amylovora, levan and amylovoran, were determined. Transposon mutants lacking levan synthesis were fully virulent on immature pears and showed retarded fireblight symptoms on shoots of pear s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63c160fcdc7a497a08b85ce513a1ae63
http://www.actahort.org/books/338/338_39.htm
http://www.actahort.org/books/338/338_39.htm
Publikováno v:
Applied and Environmental Microbiology
Detection and identification of the fire blight pathogen, Erwinia amylovora, can be accurately done by polymerase chain reaction (PCR) analysis in less than 6 h. Two oligomers derived from a 29-kb plasmid which is common to all strains of E. amylovor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d500459d8245ad9ca5e7e28233960c60
http://www.ncbi.nlm.nih.gov/pubmed/1482178
http://www.ncbi.nlm.nih.gov/pubmed/1482178
Publikováno v:
Nucleic Acids Research (London)
The suicide plasmid pfdA31-Tn5 was constructed to mutagenize Erwinia amylovora and Escherichia coli strains by electorporation. This vector carries the bacteriophage fd replication origin, a beta-lactamase gene and the transposon Tn5. For propagation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d29ced6a4162228b88a73555ceea43c1
https://pubmed.ncbi.nlm.nih.gov/1317549
https://pubmed.ncbi.nlm.nih.gov/1317549