Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Peter A. Jekel"'
Autor:
Hein J. Wijma, Hesam Arabnejad, Elvira Bombino, Dick B. Janssen, Peter A. Jekel, Milos Trajkovic, Dana I. Colpa
Publikováno v:
ChemBiochem
Chembiochem
ChemBioChem, 21(13), 1893-1904. WILEY-V C H VERLAG GMBH
Chembiochem
ChemBioChem, 21(13), 1893-1904. WILEY-V C H VERLAG GMBH
The use of enzymes in preparative biocatalysis often requires tailoring enzyme selectivity by protein engineering. Herein we explore the use of computational library design and molecular dynamics simulations to create variants of limonene epoxide hyd
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51245dbf58689846b88f77c9465aa79f
Autor:
Anke C. Terwisscha van Scheltinga, Dick B. Janssen, Peter A. Jekel, Bauke W. Dijkstra, Robert J. Floor, Hein J. Wijma
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 83:940-951
Protein engineering aimed at enhancing enzyme stability is increasingly supported by computational methods for calculation of mutant folding energies and for the design of disulfide bonds. To examine the accuracy of mutant structure predictions under
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c3bcd5ca0a45e3c87be624e48ce6db52
https://doi.org/10.1002/prot.24791
https://doi.org/10.1002/prot.24791
Autor:
Maja Majerić Elenkov, Peter A. Jekel, Bernhard Hauer, Hans Wolfgang Höffken, Dick B. Janssen, M. Schallmey, Lixia Tang
Publikováno v:
Enzyme and Microbial Technology, 70, 50-57. ELSEVIER SCIENCE INC
The cyanide-mediated ring opening of epoxides catalyzed by halohydrin dehalogenases yields β-hydroxynitriles that are of high interest for synthetic chemistry. The best studied halohydrin dehalogenase to date is the enzyme from Agrobacterium radioba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8e42f0e4c50e407758be6cc89c63abb
https://doi.org/10.1016/j.enzmictec.2014.12.009
https://doi.org/10.1016/j.enzmictec.2014.12.009
Autor:
Dick B. Janssen, Ben L. Feringa, Siewert J. Marrink, Aline Ramos-Silva, Hein J. Wijma, Wiktor Szymanski, Peter A. Jekel, Robert J. Floor, Dana I. Colpa
Publikováno v:
ChemBioChem, 15(11), 1659-1671. WILEY-V C H VERLAG GMBH
We explored the use of a computational design framework for the stabilization of the haloalkane dehalogenase LinB. Energy calculations, disulfide bond design, molecular dynamics simulations, and rational inspection of mutant structures predicted many
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::061cfef9e51a69fbec0254ec823b7cf0
https://doi.org/10.1002/cbic.201402128
https://doi.org/10.1002/cbic.201402128
Autor:
Siewert J. Marrink, Robert J. Floor, David Baker, Peter A. Jekel, Hein J. Wijma, Dick B. Janssen
Publikováno v:
Protein Engineering, Design and Selection
Protein Engineering, Design & Selection, 27(2), 49-58
Protein Engineering, Design & Selection, 27(2), 49-58
The ability to engineer enzymes and other proteins to any desired stability would have wide-ranging applications. Here, we demonstrate that computational design of a library with chemically diverse stabilizing mutations allows the engineering of dras
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac8fafa653efef17d129095bfee1ce7b
https://doi.org/10.1093/protein/gzt061
https://doi.org/10.1093/protein/gzt061
Autor:
Marco Dal Lago, Hein J. Wijma, Anke C. Terwisscha van Scheltinga, Robert J. Floor, Peter A. Jekel, Dick B. Janssen, Andy-Mark W. H. Thunnissen, Hesam Arabnejad
Publikováno v:
Protein Engineering, Design & Selection, 30(3), 175-189
To improve the applicability of halohydrin dehalogenase as a catalyst for reactions in the presence of organic cosolvents, we explored a computational library design strategy (Framework for Rapid Enzyme Stabilization by Computational libraries) that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8beb3948e99e1af8442e68a156810844
https://doi.org/10.1093/protein/gzw068
https://doi.org/10.1093/protein/gzw068
Autor:
Peter A. Jekel, Robert J. Floor, Bernhard Hauer, Dick B. Janssen, Bauke W. Dijkstra, M. Schallmey, Hein J. Wijma, Michael Breuer
Publikováno v:
ChemBioChem, 14(7), 870-881. WILEY-V C H VERLAG GMBH
Two highly engineered halohydrin dehalogenase variants were characterized in terms of their performance in dehalogenation and epoxide cyanolysis reactions. Both enzyme variants outperformed the wild-type enzyme in the cyanolysis of ethyl (S)-3,4-epox
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f42e041ccccded5746c48615cef9d89e
https://doi.org/10.1002/cbic.201300005
https://doi.org/10.1002/cbic.201300005
Publikováno v:
FEBS Letters, 585(6), 893-898. Wiley
Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::543c551226fa5566da5a359a459e2d22
https://research.rug.nl/en/publications/c1e2e308-6068-4ffc-8522-fc479945342e
https://research.rug.nl/en/publications/c1e2e308-6068-4ffc-8522-fc479945342e
Autor:
Martijn J. Koetsier, Roel A. L. Bovenberg, Dick B. Janssen, Marco A. van den Berg, Peter A. Jekel
Publikováno v:
Biochemical Journal, 417, 467-476. PORTLAND PRESS LTD
Enzymatic activation of PAA (phenylacetic acid) to phenylacetyl-CoA is an important step in the biosynthesis of the beta-lactam antibiotic penicillin G by the fungus Penicillium chrysogenum. CoA esters of PAA and POA (phenoxyacetic acid) act as acyl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ff79f1024d4910c1626a1a259807abc
https://doi.org/10.1042/bj20081257
https://doi.org/10.1042/bj20081257
Publikováno v:
Enzyme and Microbial Technology, 40(5), 1335-1344. ELSEVIER SCIENCE INC
Penicillin acylase (PA) from Escherichia coli can catalyze the acylation of 6-aminopenicillanic acid (6-APA), a conversion that is applicable in the biocatalytic preparation of semi-synthetic β-lactam antibiotics such as ampicillin. The efficiency o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a68e148119ebecb99119828c9460210d
https://doi.org/10.1016/j.enzmictec.2006.10.014
https://doi.org/10.1016/j.enzmictec.2006.10.014