Synthetic single domain antibodies for the conformational trapping of membrane proteins

Autor: Iwan Zimmermann, Pascal Egloff, Cedric AJ Hutter, Fabian M Arnold, Peter Stohler, Nicolas Bocquet, Melanie N Hug, Sylwia Huber, Martin Siegrist, Lisa Hetemann, Jennifer Gera, Samira Gmür, Peter Spies, Daniel Gygax, Eric R Geertsma, Roger JP Dawson, Markus A Seeger

Publikováno v: eLife, Vol 7 (2018)

Mechanistic and structural studies of membrane proteins require their stabilization in specific conformations. Single domain antibodies are potent reagents for this purpose, but their generation relies on immunizations, which impedes selections in th

Externí odkaz: https://doaj.org/article/0128ad13246b41d488888b761db2ce68

Structural insights into the inhibition of glycine reuptake

Autor: Wolfgang Guba, Markus A. Seeger, Azadeh Shahsavar, Iwan Zimmermann, Thomas R. Schneider, Steffen Sinning, Gleb Bourenkov, Emmanuel Pinard, Poul Nissen, Peter Stohler, Martin Siegrist, Roger J. P. Dawson

Publikováno v: Shahsavar, A, Stohler, P, Bourenkov, G, Zimmermann, I, Siegrist, M, Guba, W, Pinard, E, Sinning, S, Seeger, M A, Schneider, T R, Dawson, R J P & Nissen, P 2021, ' Structural insights into the inhibition of glycine reuptake ', Nature, vol. 591, pp. 677-681 . https://doi.org/10.1038/s41586-021-03274-z

The human glycine transporter 1 (GlyT1) regulates glycine-mediated neuronal excitation and inhibition through the sodium- and chloride-dependent reuptake of glycine1–3. Inhibition of GlyT1 prolongs neurotransmitter signalling, and has long been a k

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e5fbb4ec10167844f6037a4d2725e07
https://pubmed.ncbi.nlm.nih.gov/33658720

Identifying Conformation-Selective Heavy-Chain-Only Antibodies Against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay

Autor: Peter Stohler, Roger J. P. Dawson

Publikováno v: Methods in Molecular Biology ISBN: 9781071603727

Over the last decades, the use of heavy-chain-only antibodies has received growing attention in academia and industry as research and diagnostic tools as well as therapeutics. Their generation has improved with the help of innovative new methods such

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2e3c08c435cfb303d82f0d6eaef79838
https://doi.org/10.1007/978-1-0716-0373-4_13

Author response: Synthetic single domain antibodies for the conformational trapping of membrane proteins

Autor: Markus A. Seeger, Melanie N. Hug, Iwan Zimmermann, Peter Stohler, Daniel Gygax, Sylwia Huber, Martin Siegrist, Nicolas Bocquet, Roger J. P. Dawson, Jennifer Gera, Lisa Hetemann, Fabian M. Arnold, Eric R. Geertsma, Pascal Egloff, Samira Gmür, Peter Spies, Cedric A. J. Hutter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::031199483f49a8e6fbc737aa3ceae4be
https://doi.org/10.7554/elife.34317.040

Biosynthetic Origin of a Branched Chain Analogue of the Lipase Inhibitor, Lipstatin

Autor: Adelbert Bacher, Wolfgang Weber, Ernst Kupfer, Peter Stohler, Wolfgang Eisenreich

Publikováno v: Journal of Medicinal Chemistry. 46:4209-4212

The lipophilic beta-lactone, lipstatin, inhibits pancreatic lipase and has been shown earlier to be biosynthesized by Claisen condensation of two fatty acid moieties. We present data from incorporation experiments with [U-13C6]leucine showing that a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3800a8626a195a664e199d1ded08dcef
https://doi.org/10.1021/jm030780x