Novel peptide ligand with high binding capacity for antibody purification

Autor: Gunnar Houen, Phaedria M. St. Hilaire, Martin Lund, Leif Nørskov-Lauritsen, N. Johan V. Lindgren, Per-Erik Gustavsson, Roice Michael, Line Naomi Lund, Arne Staby

Publikováno v: Lund, L N, Gustavsson, P E, Michael, R, Lindgren, J, Norskov-Lauritsen, L, Lund, M, Houen, G, Staby, A & St Hilaire, P M 2012, ' Novel peptide ligand with high binding capacity for antibody purification ', Journal of Chromatography A, vol. 1225, pp. 158-167 . https://doi.org/10.1016/j.chroma.2011.12.074

Small synthetic ligands for protein purification have become increasingly interesting with the growing need for cheap chromatographic materials for protein purification and especially for the purification of monoclonal antibodies (mAbs). Today, Prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59d356e9bc8cef7e9490a8df0e43f3d7
https://doi.org/10.1016/j.chroma.2011.12.074

Integrated process for purification of plasmid DNA using aqueous two-phase systems combined with membrane filtration and lid bead chromatography

Autor: Tomas Nyhammar, Per-Erik Gustavsson, Raf Lemmens, Jozsef Vasi, Cecilia Kepka

Publikováno v: Journal of Chromatography A. 1057:115-124

An integrated process for purifying a 6.1 kilo base pair (kbp) plasmid from a clarified Escherichia coli cell lysate based on an ultra/diafiltration step combined with polymer/polymer aqueous two-phase system and a new type of chromatography is descr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a980ff14bc6a6f15c805876ac4cf7fa
https://doi.org/10.1016/j.chroma.2004.09.060

Purification of plasmid DNA with a new type of anion-exchange beads having a non-charged surface

Autor: Per-Olof Larsson, Tomas Nyhammar, Per-Erik Gustavsson, Raf Lemmens, Philippe Busson

Publikováno v: Journal of Chromatography A. 1038:131-140

We have prepared a new type of anion exchanger, which effectively discriminates between RNA and plasmid DNA. The material is based on a Sephacryl S-500 HR matrix provided with quartenary amine anion-exchange groups. A distinguishing feature of the be

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7a395d9d78f8916bf8e71bf53c0bfd8
https://doi.org/10.1016/j.chroma.2004.03.050

Factors Controlling Phase Separation in Water−Salt Solutions of DNA and Polycations

Autor: Igor Yu. Galaev, Per-Erik Gustavsson, Per-Olof Wahlund, Per-Olof Larsson, Vladimir A. Izumrudov

Publikováno v: Langmuir. 19:4733-4739

Factors affecting phase separation in water-salt solutions of polyelectrolyte complexes (PECs), formed by DNA and integral or pendant polycations with a quaternary amino group in every monomer unit, have been studied. When no salt was added, quantita

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https://doi.org/10.1021/la0206256

Continuous superporous agarose beds in radial flow columns

Autor: Per-Erik Gustavsson, Per-Olof Larsson

Publikováno v: Journal of Chromatography A. 925:69-78

Continuous superporous agarose beds constitute a new support material for chromatography, biocatalysis and electrophoresis. The bed consists of a single piece of agarose gel, homogeneously transected by flow-carrying pores, which easily can be varied

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::926ff6cc6e0ee9c0729b9ddbadd62171
https://doi.org/10.1016/s0021-9673(01)01027-5