Polyamine–Oligonucleotide Conjugates: 2′-OMe-Triazole-Linked 1,4,7,10-Tetraazacyclododecane and Intercalating Dyes and Their Effect on the Thermal Stability of DNA Duplexes

Autor: Mateusz D. Tomczyk, Mariusz Zalewski, Per T. Jørgensen, Jesper Wengel, Krzysztof Walczak

Publikováno v: Pharmaceutics, Vol 14, Iss 1, p 66 (2021)

Oligonucleotides with the sequences 5′-GTG AUPA TGC, 5′-GCA TAUP CAC and 5′-GUPG ATA UPGC, where UP is 2′-O-propargyl uridine, were subjected to post-synthetic Cu(I)-catalyzed azide–alkyne cycloaddition to attach 1,4,7,10-tetraazacyclododec

Externí odkaz: https://doaj.org/article/34036697d7ef4c3aa0cc0d33123225df

Evaluation of Gene Expression Knock-Down by Chemically and Structurally Modified Gapmer Antisense Oligonucleotides

Autor: Jolanta Lisowiec‐Wąchnicka, Mathias B. Danielsen, Eugenie Abi Nader, Per T. Jørgensen, Jesper Wengel, Anna Pasternak

Publikováno v: Chembiochem : a European journal of chemical biology. 23(15)

We analyzed the effect of modified nucleotides within gapmer antisense oligonucleotides on RNase H mediated gene silencing. Additionally, short hairpins were introduced into antisense oligonucleotides as structural motifs, and their influence on biol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67d0427e597e47dbae1cb59fc4e62993
https://pubmed.ncbi.nlm.nih.gov/35675170

Antisense locked nucleic acid gapmers to control Candida albicans filamentation

Autor: Maria Elisa Rodrigues, Dalila Mil-Homens, Jesper Wengel, Daniela Araújo, Per T. Jørgensen, Mariana Henriques, Sónia Carina Silva

Publikováno v: Araújo, D, Mil-Homens, D, Rodrigues, M E, Henriques, M, Jørgensen, P T, Wengel, J & Silva, S 2022, ' Antisense locked nucleic acid gapmers to control Candida albicans filamentation ', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 39, 102469 . https://doi.org/10.1016/j.nano.2021.102469
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

Whereas locked nucleic acid (LNA) has been extensively used to control gene expression, it has never been exploited to control Candida virulence genes. Thus, the main goal of this work was to compare the efficacy of five different LNA-based antisense

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40e9c0c0dc22aab0e0cd11b7f06eaebe
https://hdl.handle.net/1822/74493

Polyamine‐Functionalized 2′‐Amino‐LNA in Oligonucleotides:Facile Synthesis of New Monomers and High‐Affinity Binding towards ssDNA and dsDNA

Autor: Niels Johan Christensen, Chenguang Lou, Knud J. Jensen, Jesper Wengel, Per T. Jørgensen, Mathias B. Danielsen

Publikováno v: Danielsen, M B, Christensen, N J, Jørgensen, P T, Jensen, K J, Wengel, J & Lou, C 2021, ' Polyamine-Functionalized 2′-Amino-LNA in Oligonucleotides : Facile Synthesis of New Monomers and High-Affinity Binding towards ssDNA and dsDNA ', Chemistry: A European Journal, vol. 27, no. 4, pp. 1416-1422 . https://doi.org/10.1002/chem.202004495
Danielsen, M B, Christensen, N J, Jørgensen, P T, Jensen, K J, Wengel, J & Lou, C 2021, ' Polyamine-Functionalized 2′-Amino-LNA in Oligonucleotides : Facile Synthesis of New Monomers and High-Affinity Binding towards ssDNA and dsDNA ', Chemistry-A European Journal, vol. 27, no. 4, pp. 1416-1422 . https://doi.org/10.1002/chem.202004495

Attachment of cationic moieties to oligonucleotides (ONs) promises not only to increase the binding affinity of antisense ONs by reducing charge repulsion between the two negatively charged strands of a duplex, but also to augment their in vivo stabi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acafae1eb3e7bb21a15c28964221b965
https://curis.ku.dk/ws/files/334664969/Polyamine_functionalized_2_10.pdf

Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers

Autor: Jesper Wengel, Per T. Jørgensen, Alaa S. Gouda, Asmaa Abdelrahman

Publikováno v: Nucleosides, Nucleotides & Nucleic Acids
Abdelrahman, A, Gouda, A S, Jørgensen, P T & Wengel, J 2020, ' Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers ', Nucleosides, Nucleotides and Nucleic Acids, vol. 39, no. 1-3, pp. 82-96 . https://doi.org/10.1080/15257770.2019.1683188

This is the first report exploring the capability of twisted intercalating nucleic acid (TINA) and naphthalene-functionalized non-nucleosidic linkers to stabilize and engage in double-helical structures. Four designs were studied with respect to the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6724e79700c77358ed87111b0eecbf98