The functional role of the autolysis loop in the regulation of factor X upon hemostatic response

Autor: Amalie Carnbring Bonde, Jacob Lund, Jens Jacob Hansen, Jakob Rahr Winther, Per Franklin Nielsen, Stefan Zahn, Peter Tiainen, Ole Hvilsted Olsen, Helle Heibroch Petersen, Jais Rose Bjelke

Publikováno v: Bonde, A C, Lund, J, Hansen, J J, Winther, J R, Nielsen, P F, Zahn, S, Tiainen, P, Olsen, O H, Petersen, H H & Rose Bjelke, J 2022, ' The functional role of the autolysis loop in the regulation of factor X upon hemostatic response ', Journal of Thrombosis and Haemostasis, vol. 20, no. 3, pp. 589-599 . https://doi.org/10.1111/jth.15624
Carnbring Bonde, A, Lund, J, Hansen, J J, Winter, J R, Franklin Nielsen, P, Zahn, S, Tiainen, P, Hvilsted Olsen, O, Heibroch Petersen, H & Rose Bjelke, J 2022, ' The functional role of the autolysis loop in the regulation of Factor X upon hemostatic response ', Journal of Thrombosis and Haemostasis, vol. 20, no. 3, pp. 589-599 . https://doi.org/10.1111/jth.15624

BACKGROUND: The regulation of Factor X (FX) is critical to maintain the balance between blood coagulation and fluidity.OBJECTIVES: To functionally characterize the role of the FX autolysis loop in the regulation of the zymogen and active form of FX.M

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d96f2c6bd4cd40f5ca6318aa87b94130
https://curis.ku.dk/ws/files/305539259/PELU_J_of_Thrombosis_Haemost_2021_Bonde_The_functional_role_of_the_autolysis_loop_in_the_regulation_of_Factor_X_upon.pdf

Disulphide-mediated site-directed modification of proteins

Autor: Milan Štengl, Emiliano Cló, Zuzana Drobňáková, Anders Märcher, Vojtěch Balšánek, Thomas E. Nielsen, Michal Hučko, Per Franklin Nielsen, Thorbjørn B. Nielsen, Charlotte Wiberg, Kurt V. Gothelf

Publikováno v: Nielsen, T, Märcher, A, Drobňáková, Z, Hučko, M, Štengl, M, Balšánek, V, Wiberg, C, Nielsen, P F, Nielsen, T E, Gothelf, K V & Cló, E 2020, ' Disulphide-mediated site-directed modification of proteins ', Organic and Biomolecular Chemistry, vol. 18, no. 25, pp. 4717-4722 . https://doi.org/10.1039/d0ob00861c

Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::089d8488bcff0408e6478852817af22b
https://pure.au.dk/portal/da/publications/disulphidemediated-sitedirected-modification-of-proteins(fd0ae506-7b58-4c53-a66c-17e7df3310c1).html

Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM

Autor: Thomas E. Nielsen, Michael Rosholm Mortensen, Anne Louise Bank Kodal, Emiliano Cló, Jørgen Kjems, Per Franklin Nielsen, Kurt V. Gothelf, Thorbjørn B. Nielsen, Rasmus P. Thomsen

Publikováno v: Nielsen, T B, Thomsen, R P, Mortensen, M R, Kjems, J, Nielsen, P F, Nielsen, T E, Kodal, A L B, Cló, E & Gothelf, K V 2019, ' Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM ', Angewandte Chemie International Edition, vol. 58, no. 27, pp. 9068-9072 . https://doi.org/10.1002/anie.201903134

The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnish

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06d6e156677fa7259bda877687313cda
https://pubmed.ncbi.nlm.nih.gov/30995340

Structure−Activity and Protraction Relationship of Long-Acting Glucagon-like Peptide-1 Derivatives: Importance of Fatty Acid Length, Polarity, and Bulkiness

Autor: Per Franklin Nielsen, N. L. Johansen, Kjeld Madsen, Lotte Bjerre Knudsen, Henrik Agersoe, Henning Thøgersen, Michael Wilken

Publikováno v: Journal of Medicinal Chemistry. 50:6126-6132

We here report a series of derivatives describing the structure-activity relationship around liraglutide, a once-daily human glucagon-like peptide-1 fatty acid derivative, with respect to potency as well as protraction in vivo. The spacer region betw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d56f5c7bd00b8530ddcbf29aae58002
https://doi.org/10.1021/jm070861j

Antimicrobial peptide defenses of the mountain yellow-legged frog (Rana muscosa)

Autor: J. Michael Conlon, Per Franklin Nielsen, Cheryl J. Briggs, Louise A. Rollins-Smith, Vance T. Vredenburg, Douglas C. Woodhams, Laura K. Reinert

Publikováno v: Developmental & Comparative Immunology. 30:831-842

The mountain yellow-legged frog (Rana muscosa) inhabits high elevation lakes in California that are largely undisturbed by human activities. In spite of this habitation in remote sites, populations continue to decline. Although predation by non-nativ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::205503264bf4b4388e5c274080c80ce9
https://doi.org/10.1016/j.dci.2005.10.005