Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Per Borup-Christensen"'
Publikováno v:
Cancer. 73:858-863
The human monoclonal immunoglobulin M (IgM) antibody, COU-1 is obtained from a human-human hybridoma, which is derived by fusion between a human B-lymphoblastoid cell line and lymphocytes obtained from mesenteric lymph nodes from a patient with color
Autor:
Per Borup-Christensen, Helmuth Haas, Henrik J. Ditzel, John Chemnitz, Karin Erb, Jens C. Jensenius
Publikováno v:
Scopus-Elsevier
Erb, K, Borup-Christensen, P, Ditzel, H, Chemnitz, J, Haas, H & Jensenius, J C 1992, ' Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen ', Hybridoma, vol. 11, no. 2, pp. 121-34 . https://doi.org/10.1089/hyb.1992.11.121
Erb, K, Borup-Christensen, P, Ditzel, H, Chemnitz, J, Haas, H D & Jensenius, J C 1992, ' Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen ' Hybridoma, vol. 11, no. 2, pp. 121-34 .
Erb, K, Borup-Christensen, P, Ditzel, H, Chemnitz, J, Haas, H & Jensenius, J C 1992, ' Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen ', Hybridoma, vol. 11, no. 2, pp. 121-34 . https://doi.org/10.1089/hyb.1992.11.121
Erb, K, Borup-Christensen, P, Ditzel, H, Chemnitz, J, Haas, H D & Jensenius, J C 1992, ' Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen ' Hybridoma, vol. 11, no. 2, pp. 121-34 .
The human hybridoma cell line, B9165, was obtained after fusion of lymphocytes from lymph nodes draining the tumor region in a patient with adenocarcinoma of the colon with the human B-lymphoblastoid cell line WI-L2-729-HF2 (729-HF2). B9165 secretes
Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165)
Publikováno v:
Erb, K, Ditzel, H, Waever-Rasmussen, J, Borup-Christensen, P & Jensenius, J C 1991, ' Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165) ', Human antibodies and hybridomas, vol. 2, no. 4, pp. 215-21 .
Erb, K, Ditzel, H, Waever-Rasmussen, J, Borup-Christensen, P & Jensenius, J C 1991, ' Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165) ' Human antibodies and hybridomas, vol. 2, no. 4, pp. 215-21 .
Scopus-Elsevier
Europe PubMed Central
Erb, K, Ditzel, H, Waever-Rasmussen, J, Borup-Christensen, P & Jensenius, J C 1991, ' Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165) ' Human antibodies and hybridomas, vol. 2, no. 4, pp. 215-21 .
Scopus-Elsevier
Europe PubMed Central
We have compared by SDS-PAGE Western blotting the molecules detected by two human monoclonal antibodies, C-OU1 and 16.88. The antibodies have previously been shown to detect a cytoplasmatic antigen with an Mr of 43 kD present in colon adenocarcinoma
Publikováno v:
Ditzel, H, Erb, K, Borup-Christensen, P, Nielsen, B & Jensenius, J C 1991, ' Evaluation of procedures for the fixation and processing of human tissue for immunohistochemical analysis of human monoclonal antibodies ', Human Antibodies, vol. 2, no. 3, pp. 135-141 . https://doi.org/10.3233/HAB-1991-2303
Scopus-Elsevier
Ditzel, H, Erb, K, Borup-Christensen, P, Nielsen, B & Jensenius, J C 1991, ' Evaluation of procedures for the fixation and processing of human tissue for immunohistochemical analysis of human monoclonal antibodies ' Human antibodies and hybridomas, vol. 2, no. 3, pp. 135-41 .
Europe PubMed Central
Scopus-Elsevier
Ditzel, H, Erb, K, Borup-Christensen, P, Nielsen, B & Jensenius, J C 1991, ' Evaluation of procedures for the fixation and processing of human tissue for immunohistochemical analysis of human monoclonal antibodies ' Human antibodies and hybridomas, vol. 2, no. 3, pp. 135-41 .
Europe PubMed Central
Preliminary investigations suggested the importance of an evaluation of different tissue preparation methods frequently used for immunohistochemical analysis of human or murine monoclonal antibodies on human tissue. Colon adenocarcinomas and adjacent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41da8cacd9c3ca0bcfeb3987ec7751ec
https://portal.findresearcher.sdu.dk/da/publications/9d243d8e-7e2c-4ae7-aebb-cbcd47960a0f
https://portal.findresearcher.sdu.dk/da/publications/9d243d8e-7e2c-4ae7-aebb-cbcd47960a0f
Publikováno v:
Ditzel, H, Erb, K, Nielsen, B, Borup-Christensen, P & Jensenius, J C 1990, ' A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies ', Journal of Immunological Methods, vol. 133, no. 2, pp. 245-51 . https://doi.org/10.1016/0022-1759(90)90365-3
Ditzel, H, Erb, K, Nielsen, B, Borup-Christensen, P & Jensenius, J C 1990, ' A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies ' Journal of Immunological Methods, vol. 133, no. 2, pp. 245-51 .
Ditzel, H, Erb, K, Nielsen, B, Borup-Christensen, P & Jensenius, J C 1990, ' A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies ' Journal of Immunological Methods, vol. 133, no. 2, pp. 245-51 .
Using an indirect immunoperoxidase technique antigen independent binding of both human monoclonal and polyclonal IgM was found to a wide range of frozen sections of normal and malignant human glandular epithelia. Identical binding was found using dim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e33c06ad3d22cf4bca40d637f650e91b
https://portal.findresearcher.sdu.dk/da/publications/f6b643ab-8670-4e08-a48e-1d98c46d880b
https://portal.findresearcher.sdu.dk/da/publications/f6b643ab-8670-4e08-a48e-1d98c46d880b
Autor:
Karin Erb, Henrik J. Ditzel, Jens Chr. Jensenius, Per Borup-Christensen, Boye Schnack Nielsen, Børge Teisner
Publikováno v:
Ditzel, H, Erb, K, Teisner, B, Nielsen, B, Borup-Christensen, P & Jensenius, J C 1990, ' Immunohistochemical analysis of the binding of human IgM to secretory component present in normal adult colon epithelia, but not in colon cancer and fetal colon epithelia ' Histochemistry, vol. 94, no. 1, pp. 95-9 .
Ditzel, H, Erb, K, Teisner, B, Nielsen, B, Borup-Christensen, P & Jensenius, J C 1990, ' Immunohistochemical analysis of the binding of human IgM to secretory component present in normal adult colon epithelia, but not in colon cancer and fetal colon epithelia ', Histochemistry, vol. 94, no. 1, pp. 95-99 . https://doi.org/10.1007/BF00266795
Ditzel, H, Erb, K, Teisner, B, Nielsen, B, Borup-Christensen, P & Jensenius, J C 1990, ' Immunohistochemical analysis of the binding of human IgM to secretory component present in normal adult colon epithelia, but not in colon cancer and fetal colon epithelia ', Histochemistry, vol. 94, no. 1, pp. 95-99 . https://doi.org/10.1007/BF00266795
We have analysed the binding of human IgM to fetal, normal adult and malignant colo-rectal tissues. Using an indirect immunoperoxidase technique on sections of frozen tissues human IgM binds to all normal adult colo-rectal epithelia (n=15) tested. By
Autor:
Karin Erb, Jørgen K. Larsen, Jens C. Jensenius, Henrik J. Ditzel, Bjarne Nielsen, Per Borup-Christensen, S.‐E. Svehag
Publikováno v:
Borup-Christensen, P, Erb, K, Ditzel, H, Nielsen, B, Larsen, J K, Svehag, S E & Jensenius, J C 1990, ' Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens ' A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, vol. 98, no. 8, pp. 674-84 .
Europe PubMed Central
Scopus-Elsevier
Borup-Christensen, P, Erb, K, Ditzel, H, Nielsen, B, Larsen, J K, Svehag, S E & Jensenius, J C 1990, ' Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens ', APMIS, vol. 98, no. 8, pp. 674-684 . https://doi.org/10.1111/j.1699-0463.1990.tb04988.x
Europe PubMed Central
Scopus-Elsevier
Borup-Christensen, P, Erb, K, Ditzel, H, Nielsen, B, Larsen, J K, Svehag, S E & Jensenius, J C 1990, ' Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens ', APMIS, vol. 98, no. 8, pp. 674-684 . https://doi.org/10.1111/j.1699-0463.1990.tb04988.x
Lymphocytes from lymph nodes draining the tumor region in patients with colorectal cancer were fused with two different human B-lymphoblastoid cell lines, LICR-LON-HMy-2 (HMy-2) and WI-L2-729-HF2 (729-HF2), to generate hybridomas synthesizing antibod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cd071c99e4744a5fa526b1795edb5f6
https://pure.au.dk/portal/da/publications/humanhuman-hybridoma-producing-monoclonal-antibodies-against-colorectal-cancerassociated-antigens(684d1c8d-a4d0-48a1-8cae-fc5006cd28e0).html
https://pure.au.dk/portal/da/publications/humanhuman-hybridoma-producing-monoclonal-antibodies-against-colorectal-cancerassociated-antigens(684d1c8d-a4d0-48a1-8cae-fc5006cd28e0).html
Publikováno v:
Erb, K, Borup-Christensen, P, Jensenius, J C, Svehag, S-E & Nielsen, B 1986, ' Human-human hybridomas for the study of anti-tumor immune response in patients with colorectal cancer ', International Journal of Cancer, vol. 37, no. 5, pp. 683-688 . https://doi.org/10.1002/ijc.2910370507
Borup-Christensen, P, Erb, K, Jensenius, J C, Nielsen, B & Svehag, S E 1986, ' Human-human hybridomas for the study of anti-tumor immune response in patients with colorectal cancer ' International Journal of Cancer, vol. 37, no. 5, pp. 683-8 .
Borup-Christensen, P, Erb, K, Jensenius, J C, Nielsen, B & Svehag, S E 1986, ' Human-human hybridomas for the study of anti-tumor immune response in patients with colorectal cancer ' International Journal of Cancer, vol. 37, no. 5, pp. 683-8 .
Human-human hybridoma technology was evaluated for the study of humoral immune reactions of colorectal cancer patients against their own tumors. Six fusions were carried out with lymphocytes from mesenteric lymph nodes from patients with colorectal c
Publikováno v:
Nielsen, B, Borup-Christensen, P, Erb, K, Jensenius, J C & Husby, S 1987, ' A method for the blocking of endogenous immunoglobulin on frozen tissue sections in the screening of human hybridoma antibody in culture supernatants ' Hybridoma, vol. 6, no. 1, pp. 103-9 .
Endogenous immunoglobulin in tissue sections pose a problem in immuno-histochemical techniques employing homologous antibody as primary reagents and enzyme-labelled anti-immunoglobulin for the development. A method for the blocking of endogenous immu
Publikováno v:
Borup-Christensen, P, Erb, K & Jensenius, J C 1988, ' Curing human hybridomas infected with Mycoplasma hyorhinis ' Journal of Immunological Methods, vol. 110, no. 2, pp. 237-40 .
Tiamuline and minocycline were evaluated for the treatment of an IgM producing human-human hybridoma cell line infected with Mycoplasma hyorhinis. Tiamuline was used at a concentration of 10 micrograms/ml culture medium and minocycline at a concentra