Zobrazeno 1 - 10
of 53
pro vyhledávání: '"Pehr B Harbury"'
Publikováno v:
eLife, Vol 7 (2018)
A major challenge in biology is identifying distinct cell classes and mapping their interactions in vivo. Tissue-dissociative technologies enable deep single cell molecular profiling but do not provide spatial information. We developed a proximity li
Externí odkaz:
https://doaj.org/article/a01cf8c83f4f4b8e91adef19dfa5f9a1
Publikováno v:
PLoS ONE, Vol 11, Iss 8, p e0154765 (2016)
The first demonstration that macromolecules could be evolved in a test tube was reported twenty-five years ago. That breakthrough meant that billions of years of chance discovery and refinement could be compressed into a few weeks, and provided a pow
Externí odkaz:
https://doaj.org/article/bc7811ef17ab482d93508cf3639a486d
Publikováno v:
PLoS ONE, Vol 7, Iss 3, p e28056 (2012)
A large body of in vitro evolution work establishes the utility of biopolymer libraries comprising 10(10) to 10(15) distinct molecules for the discovery of nanomolar-affinity ligands to proteins. Small-molecule libraries of comparable complexity will
Externí odkaz:
https://doaj.org/article/109b79177d004117b5e66454be7e4790
Publikováno v:
PLoS ONE, Vol 7, Iss 3, p e32299 (2012)
Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful tools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical libra
Externí odkaz:
https://doaj.org/article/45964417a95c44fdbe94893543db6d37
Publikováno v:
PLoS Biology, Vol 2, Iss 7, p E175 (2004)
DNA-directed synthesis represents a powerful new tool for molecular discovery. Its ultimate utility, however, hinges upon the diversity of chemical reactions that can be executed in the presence of unprotected DNA. We present a solid-phase reaction f
Externí odkaz:
https://doaj.org/article/757b9934d7b44cd497ebbb11786bfa8c
Autor:
David R Halpin, Pehr B Harbury
Publikováno v:
PLoS Biology, Vol 2, Iss 7, p E173 (2004)
Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Her
Externí odkaz:
https://doaj.org/article/0de05a8065f54e97b98d5e37c1f07a5b
Autor:
David R Halpin, Pehr B Harbury
Publikováno v:
PLoS Biology, Vol 2, Iss 7, p E174 (2004)
Biological in vitro selection techniques, such as RNA aptamer methods and mRNA display, have proven to be powerful approaches for engineering molecules with novel functions. These techniques are based on iterative amplification of biopolymer librarie
Externí odkaz:
https://doaj.org/article/ddda7afd82c548baaaefbb954155b4e0
Autor:
William J. Greenleaf, Sarah A. Teichmann, Margaret Vella, Ajay Pillai, Aviv Regev, Ananda L. Roy, Kristin E. Burnum-Johnson, Cole Trapnell, Yiing Lin, Marda Jorgensen, Gloria S. Pryhuber, Leslie Gaffney, Ken S. Lau, Kimberly Robasky, Jeffrey M. Spraggins, Chuck McCallum, Stavros Michailidis, Randy Heiland, Orit Rozenblatt-Rosen, Allyson Ricarte, Xin bSun, Kevin J. Otto, Amir Bahmani, Zorina S. Galis, James S. Hagood, Monica Nagendran, Gökcen Eraslan, Robin M. Scibek, Jocelyn Y. Kishi, Jay Mulye, Maria Keays, Griffin M. Weber, Caltech-UW Tmc, Charles Ansong, Nicholas A. Nystrom, Vishal G. Venkataraaman, Vladimir Yu. Kiselev, Ucsd Tmc, Ellen M. Quardokus, Tushar bDesai, Bruce Herr, Engagement Component, James E. Anderson, Shin Lin, Lisel Record, Peter V. Kharchenko, Robert F. dMurphy, Stanford-WashU Tmc, Yu Wang, Jian Ma, Sergio Maffioletti, Sarah Black, Matthew Ruffalo, Salvatore Sechi, John C. Marioni, Ziv Bar-Joseph, Richard M. Caprioli, Stanford Ttd, Garry P. Nolan, Marishka Brown, Elizabeth L. Wilder, Maigan A. Brusko, Peter Chou, Tommaso Biancalani, Christian Martijn Schuerch, Julia Laskin, Richard Conroy, Jonathan C. Silverstein, Paula M. Mabee, Dena Procaccini, Pehr B. Harbury, Michael Snyder, Pothur Srinivas, Jennifer Rood, Dana Jackson, Sanjay Jain, Katy Börner, Visualization HuBMAP Integration, William E. Shirey, Sinem K. Saka, James P. Sluka, Agnes B. Fogo, Isabel Goldaracena, Sushma A. Akoju, Raymond C. Harris, Rahul Satija, Sylvia K. Plevritis, Tim Stuart, Shila Ghazanfar, Peng Yin, Harvard Ttd, Aaron M. Horning, Ed Esplin, Amanda Posgai, Michael J. Clare-Salzler, Raf Van de Plas, Aaron Pawlyk, Guo-Cheng Yuan, Benedict aten, DongHye Ye, Hayan Lee, Eyal Fisher, Jay Shendure, Long Cai, Danielle cGutierrez, Carl Kingsford, Ruben Dries, Sara Ahadi, Paul D. Piehowski, Bernd bBodenmiller, Purdue Ttd, Stephanie A. Nevins, Philip D. Blood, Andrew Butler, W. Christopher Lenhardt, Ying Zhu, Alexander J. Ropelewski, Harry S. Nick, Nathan Heath Patterson, Elizabeth K. Neumann, Anna Hupalowska, Samuel H. Friedman, Clive hWasserfall, Qian Zhu, Mark P. deCaestecker, Leonard E. Cross, Mark A. Atkinson, Paul Macklin, Todd M. Brusko, Eeshit Dhaval Vaishnav, Nils Gehlenborg, Kun Zhang
Publikováno v:
Nature
Transformative technologies are enabling the construction of three-dimensional maps of tissues with unprecedented spatial and molecular resolution. Over the next seven years, the NIH Common Fund Human Biomolecular Atlas Program (HuBMAP) intends to de
Publikováno v:
Bio Protoc
Understanding tissues in the context of development, maintenance and disease requires determining the molecular profiles of individual cells within their native in vivo spatial context. We developed a Proximity Ligation in situ Hybridization technolo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::761f84152664f60598ad24c3f51664e1
https://europepmc.org/articles/PMC7842654/
https://europepmc.org/articles/PMC7842654/
Autor:
Pehr B. Harbury, Grace X.Y. Zheng, Solongo B. Ziraldo, Lisa E. Wagar, Tarjei S. Mikkelsen, Manuel R. Amieva, Jihang Ju, Calvin J. Kuo, Mar Margalef-Català, Jeffrey S. Glenn, Daniel J. Hart, Mark M. Davis, Arpit Batish, Caitlin E. Edwards, Ameen A. Salahudeen, Arjun Rustagi, Catherine A. Blish, Sean M. de la O, Shannon S. Choi, Monica Nagendran, Ralph S. Baric, Vincent C. Luca, Junjie Zhu, Tushar J. Desai, Khanh Nguyen, Jessica M. Terry, Tatsuya Usui, Phillip Belgrader, Ryan A. Flynn, Vincent van Unen, Chiara Sabatti, Benedict Anchang, K. Christopher Garcia, António J. M. Santos
Publikováno v:
Nature
The distal lung contains terminal bronchioles and alveoli that facilitate gas exchange and is affected by disorders including interstitial lung disease, cancer, and SARS-CoV-2-associated COVID-19 pneumonia. Investigations of these localized pathologi