Screening for viral extraneous agents in live-attenuated avian vaccines by using a microbial microarray and sequencing

Autor: Majken Lindholm Olesen, Erik Østergaard, Merete Blixenkrone-Møller, Peer Lyng Frandsen, Eva Sandberg, Lotte Leick Jørgensen, Anders Fomsgaard, Eva Rauhe Bækdahl, Maiken Worsøe Rosenstierne, Helena Fridholm

Publikováno v: Olesen, M L, Jørgensen, L L, Blixenkrone-Møller, M, Sandberg, E, Frandsen, P L, Østergaard, E, Bækdahl, E R, Fridholm, H, Fomsgaard, A & Rosenstierne, M W 2018, ' Screening for viral extraneous agents in live-attenuated avian vaccines by using a microbial microarray and sequencing ', Biologicals, vol. 51, pp. 37-45 . https://doi.org/10.1016/j.biologicals.2017.10.005

The absence of extraneous agents (EA) in the raw material used for production and in finished products is one of the principal safety elements related to all medicinal products of biological origin, such as live-attenuated vaccines. The aim of this s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09a814eefc34de836f5bc2281faec21a
https://pubmed.ncbi.nlm.nih.gov/29132963

Development of a primer-probe energy transfer based real-time PCR for detection of Marek's disease virus

Autor: Peer Lyng Frandsen, Erik Østergaard, Annette Malene Barfoed, Thomas Bruun Rasmussen, Eva Sandberg, Eva Bækdahl Nielsen

Publikováno v: Journal of virological methods. 165(1)

A real-time PCR assay, which enables simultaneous detection and differentiation of all three serotypes of Marek's disease virus, without the need for post-PCR sequencing, has been developed. The assay is based on the primer-probe energy transfer real

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa6f7c9274d8412566db1799f88b43a3
https://pubmed.ncbi.nlm.nih.gov/20026120