Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Pedro D. Clop"'
Autor:
Ugutz Unzueta, Hèctor López-Laguna, Pedro D. Clop, Antonio Villaverde, Esther Vázquez, Julieta M. Sánchez, Naroa Serna
Publikováno v:
ACS Sustainable Chemistry & Engineering. 9:2552-2558
One of the most critical bottlenecks in the application of industrial enzymes is the preservation of protein stability throughout the catalytic reaction, which often requires protein engineering an...
Autor:
Sandra S. Flores, Pedro D. Clop, José L. Barra, Carlos Argaraña, María A. Perillo, Verónica Nolan, Julieta M. Sanchez
β-Galactosidase is one of the most important biotechnological enzyme used in the dairy industry, pharmacology and in molecular biology. In our laboratory we have overexpressed a recombinant β-galactosidase in Escherichia coli (E. coli). This enzyme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::da980eb7ca48a0e9a8a9b9bb62a03ffe
https://doi.org/10.21203/rs.3.rs-54150/v1
https://doi.org/10.21203/rs.3.rs-54150/v1
Here we studied the effect of molecular crowding on the hydrolysis of ortho- and para-nitrophenyl-β-D-galactopyranosides (ONPG, PNPG) catalysed by Escherichia coli β-Galactosidase in the presence of 0–35%w/v 6kD polyethyleneglycol (PEG6000). The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21aee565757c5c1ea38353cde30c3567
https://www.sciencedirect.com/science/article/abs/pii/S0006291X1930957X
https://www.sciencedirect.com/science/article/abs/pii/S0006291X1930957X
Publikováno v:
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PMID: 23545220 [PubMed-indexed for MEDLINE] Free full text
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PMID: 23545220 [PubMed-indexed for MEDLINE] Free full text
The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents.
Publikováno v:
The Journal of Physical Chemistry B. 116:14216-14227
The toxicity of FB1 is usually explained through the enzymatic disruption of lipidic metabolism. However, it may lie in the thermodynamics of the membrane and its cooperative phase behavior rather than in the activity of individual proteins. Here, we
Publikováno v:
Langmuir. 24:10950-10960
Functional consequences of constraining beta-Gal in bidimensional space were studied at defined molecular packing densities and constant topology. Langmuir-Blodgett films, LB15 and LB35 composed of dipalmitoyl phosphatidylcholine and K. lactis beta-G
In this work we used Langmuir-Blodgett films (LB) as model membranes to study the effect of molecular packing on the flunitrazepam (FNZ) accessibility to the binding sites at the GABAA receptor (GABAA-R). Ligand binding data were correlated with film
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e0b3e434110bb4225c278632e6bc793
https://pubs.acs.org/doi/10.1021/la5042986
https://pubs.acs.org/doi/10.1021/la5042986
Publikováno v:
Colloids and surfaces. B, Biointerfaces. 76(2)
In the present work we applied the sol-gel method to obtain glass lentils entrapping beta-D-galactosidase (beta-Gal) (Ebeta-Gal) within a silicate matrix. The effect of pH, temperature, polarity and salt concentration on the activity of Ebeta-Gal was
Publikováno v:
Langmuir; Sep2008, Vol. 24 Issue 19, p10950-10960, 11p