Zobrazeno 1 - 10
of 66
pro vyhledávání: '"Pedro Antonio García-Ruiz"'
Autor:
Pablo García-Molina, José Luis Munoz-Munoz, Joaquin A. Ortuño, José Neptuno Rodríguez-López, Pedro Antonio García-Ruiz, Francisco García-Cánovas, Francisco García-Molina
Publikováno v:
Biomolecules, Vol 11, Iss 9, p 1269 (2021)
With the purpose to obtain the more useful tyrosinase assay for the monophenolase activity of tyrosinase between the spectrofluorometric and spectrophotometric continuous assays, simulated assays were made by means of numerical integration of the equ
Externí odkaz:
https://doaj.org/article/2f8ef4f6fbb84715acdc0087b4d64461
Autor:
Jose Luis Muñoz-Muñoz, Francisco García-Cánovas, Miguel A. Maria-Solano, Jose Antonio Teruel-Puche, Carmen Vanessa Ortiz-Ruiz, Jose Berna, Pedro Antonio García-Ruiz
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 125:6-15
The pH has a great physiological effect on the catalysis of tyrosinase because protons can act as competitive-type inhibitors. In this work, a reaction mechanism is proposed whereby only the protonation of the free forms Em (met-tyrosinase) and Eox (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1405f09551c2a5e821b8b8726f80c195
https://doi.org/10.1016/j.molcatb.2015.12.008
https://doi.org/10.1016/j.molcatb.2015.12.008
Autor:
Francisco García-Cánovas, Carmen Vanessa Ortiz-Ruiz, Manuel Ballesta de los Santos, Jose Berna, José Tudela, Pedro Antonio García-Ruiz, Jose Fenoll
Publikováno v:
IUBMB Life. 67:828-836
Oxyresveratrol is a stilbenoid described as a powerful inhibitor of tyrosinase and proposed as skin-whitening and anti-browning agent. However, the enzyme is capable of acting on it, considering it as a substrate, as it has been proved in the case of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0e4d451401e13e5c6c92f106a521f56c
https://doi.org/10.1002/iub.1439
https://doi.org/10.1002/iub.1439
Autor:
Soledad Chazarra, Dorotea López-Molina, Chee Wun How, Pedro Antonio García-Ruiz, Nikolov Pruidze, Enma Navarro-Perán, José Neptuno Rodríguez-López, Francisco Rojas-Melgarejo, Francisco García-Cánovas
Publikováno v:
International Journal of Pharmaceutics. 479:96-102
Colon diseases are difficult to treat because oral administrated drugs are absorbed at the stomach and intestine levels and they do not reach colon; in addition, intravenous administrated drugs are eliminated from the body before reaching colon. Inul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48b8fa60ad8ffeb9e3d1ac814a04357b
https://doi.org/10.1016/j.ijpharm.2014.12.064
https://doi.org/10.1016/j.ijpharm.2014.12.064
Autor:
Francisco García-Cánovas, Maria del Mar Garcia-Molina, José Neptuno Rodríguez-López, Jose Luis Muñoz Muñoz, José Rodriguez Martinez, Pedro Antonio García-Ruiz, Francisco Martínez-Ortiz
Publikováno v:
Bioorganic & Medicinal Chemistry. 22:3360-3369
Hydroquinone (HQ) is used as a depigmenting agent. In this work we demonstrate that tyrosinase hydroxylates HQ to 2-hydroxyhydroquinone (HHQ). Oxy-tyrosinase hydroxylates HQ to HHQ forming the complex met-tyrosinase-HHQ, which can evolve in two diffe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc6573160eb7dcb4e31375fdfb47b68e
https://doi.org/10.1016/j.bmc.2014.04.048
https://doi.org/10.1016/j.bmc.2014.04.048
Autor:
Jose Luis Muñoz-Muñoz, Pedro Antonio García-Ruiz, Manuela Garcia Moreno, José Rodriguez Martinez, Francisco García-Cánovas, Maria del Mar Garcia-Molina, Jose Berna
Publikováno v:
Reaction Kinetics, Mechanisms and Catalysis. 112:305-320
The formation of hydroxyhydroquinone during the action of tyrosinase on hydroquinone is demonstrated for the first time by means of high performance liquid chromatography mass spectrometry. A kinetic mechanism is proposed to explain this action in th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a55d56f5ee0b0335912337fb0a04ac14
https://doi.org/10.1007/s11144-014-0723-1
https://doi.org/10.1007/s11144-014-0723-1
Autor:
José Neptuno Rodríguez-López, Francisco García-Cánovas, Maria del Mar Garcia-Molina, Jose Luis Muñoz-Muñoz, Pedro Antonio García-Ruiz, Jose Berna
Publikováno v:
IUBMB Life. 66:122-127
Hydroxyhydroquinone (HHQ) was characterized kinetically as a tyrosinase substrate. A kinetic mechanism is proposed, in which HHQ is considered as a monophenol or as an o-diphenol, depending on the part of the molecule that interacts with the enzyme.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::473ec5160c63ddfe48939bdefb53c781
https://doi.org/10.1002/iub.1250
https://doi.org/10.1002/iub.1250
Autor:
Pedro Antonio García-Ruiz, Jose Luis Muñoz-Muñoz, Maria del Mar Garcia-Molina, Francisco García-Cánovas, Francisco Garcia-Molina, Francisco García-Sevilla, José Neptuno Rodríguez-López
Publikováno v:
IUBMB Life. 65:793-799
A solvent deuterium isotope effect on the inactivation suicide of tyrosinase in its action on o-diphenols (catechol, 4-methylcatechol, and 4-tert-butylcatechol) was observed. This isotope effect, observed during kinetic studies in the transition phas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4c0f1a8ae8c36cf620a597994cb79169
https://doi.org/10.1002/iub.1191
https://doi.org/10.1002/iub.1191
Autor:
José Neptuno Rodríguez-López, Maria del Mar Garcia-Molina, Francisco Garcia-Molina, Jose Berna, Manuela García-Moreno, Pedro Antonio García-Ruiz, Francisco García-Cánovas, Jose Luis Muñoz-Muñoz
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 91:17-24
A study of the diphenolase, o-aminophenol oxidase, and the aromatic o-diamine oxidase activities of tyrosinase carried out by measuring the catalysis and suicide inactivation kinetics provides the following information: catalytic constant, k cat S ,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::512971195f96cad090307e5619b23103
https://doi.org/10.1016/j.molcatb.2013.02.003
https://doi.org/10.1016/j.molcatb.2013.02.003
Autor:
José Neptuno Rodríguez-López, Pedro Antonio García-Ruiz, Francisco García-Cánovas, Jose Antonio Teruel-Puche, Jose Berna, Antonio Garcia-Jimenez, José Tudela
Publikováno v:
Bioorganicmedicinal chemistry. 24(18)
The action of tyrosinase on resorcinol and some derivatives (4-ethylresorcinol, 2-methylresorcinol and 4-methylresorcinol) was investigated. If the catalytic cycle is completed with a reductant such as ascorbic acid or an o-diphenol such as 4-tert-bu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::775f16a7daf841837468aaa761644511
https://pubmed.ncbi.nlm.nih.gov/27480027
https://pubmed.ncbi.nlm.nih.gov/27480027