Single-Cell Profiling Reveals Divergent, Globally Patterned Immune Responses in Murine Skin Inflammation

Autor: Yale Liu, Christopher Cook, Andrew J. Sedgewick, Shuyi Zhang, Marlys S. Fassett, Roberto R. Ricardo-Gonzalez, Paymann Harirchian, Sakeen W. Kashem, Sho Hanakawa, Jacob R. Leistico, Jeffrey P. North, Mark A. Taylor, Wei Zhang, Mao-Qiang Man, Alexandra Charruyer, Nadejda Beliakova-Bethell, Stephen C. Benz, Ruby Ghadially, Theodora M. Mauro, Daniel H. Kaplan, Kenji Kabashima, Jaehyuk Choi, Jun S. Song, Raymond J. Cho, Jeffrey B. Cheng

Publikováno v: iScience, Vol 23, Iss 10, Pp 101582- (2020)

Summary: Inflammatory response heterogeneity has impeded high-resolution dissection of diverse immune cell populations during activation. We characterize mouse cutaneous immune cells by single-cell RNA sequencing, after inducing inflammation using im

Externí odkaz: https://doaj.org/article/642c47211ea24e3c8cf04b0ca1279bd0

Single-Cell Transcriptomics Reveals Spatial and Temporal Turnover of Keratinocyte Differentiation Regulators

Autor: Alex Finnegan, Raymond J. Cho, Alan Luu, Paymann Harirchian, Jerry Lee, Jeffrey B. Cheng, Jun S. Song

Publikováno v: Frontiers in Genetics, Vol 10 (2019)

Keratinocyte differentiation requires intricately coordinated spatiotemporal expression changes that specify epidermis structure and function. This article utilizes single-cell RNA-seq data from 22,338 human foreskin keratinocytes to reconstruct the

Externí odkaz: https://doaj.org/article/be88523a313548cbbd3acd462a4edf3f

scRNA-seq for chronic inflammatory skin rashes

Autor: Yale Liu, Hao Wang, Mark Taylor, Christopher Cook, Alejandra Martinez-Berdeja, Jeffrey P. North, Paymann Harirchian, Ashley A. Hailer, Zijun Zhao, Ruby Ghadially, Roberto R. Ricardo-Gonzalez, Roy C. Grekin, Theodora M. Mauro, Esther Kim, Jaehyuk Choi, Elizabeth Purdom, Raymond J Cho, Jeffrey B Cheng

In our manuscript, we utilized scRNA-seq libraries we generated (Classification of human chronic inflammatory skin disease based on single-cell immune profiling (science.org)), samples from the Reynolds et al dataset (Developmental cell programs are

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62792dea2382f14e6b9f5011af83c3f8

A single-cell transcriptional gradient in human cutaneous memory T cells restricts Th17/Tc17 identity

Autor: Christopher P. Cook, Mark Taylor, Yale Liu, Ralf Schmidt, Andrew Sedgewick, Esther Kim, Ashley Hailer, Jeffrey P. North, Paymann Harirchian, Hao Wang, Sakeen W. Kashem, Yanhong Shou, Timothy C. McCalmont, Stephen C. Benz, Jaehyuk Choi, Elizabeth Purdom, Alexander Marson, Silvia B.V. Ramos, Jeffrey B. Cheng, Raymond J. Cho

Publikováno v: Cell Reports Medicine. 3:100715

The homeostatic mechanisms that fail to restrain chronic tissue inflammation in diseases, such as psoriasis vulgaris, remain incompletely understood. We profiled transcriptomes and epitopes of single psoriatic and normal skin-resident T cells, reveal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea46d936d6654b5cedf3dfd5b39b7746
https://doi.org/10.1016/j.xcrm.2022.100715

Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution

Autor: Thaddeus W. Mully, Matthew D. Gray, Wilson Liao, Jun S. Song, Hani Sbitany, Jeffrey B. Cheng, Paymann Harirchian, Sunjong Kwon, Justin Golovato, Joe W. Gray, Roy C. Grekin, Ruby Ghadially, Marlys S. Fassett, Alex I Finnegan, Jerry Lee, Charles J. Vaske, Esther A. Kim, Elizabeth Purdom, Bethany E. Perez White, Theodora M. Mauro, Stephen C. Benz, Andrew J. Sedgewick, Siegrid S. Yu, Ralf Paus, Isaac M. Neuhaus, Raymond J. Cho

Publikováno v: Cheng, JB; Sedgewick, AJ; Finnegan, AI; Harirchian, P; Lee, J; Kwon, S; et al.(2018). Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution. Cell Reports. doi: 10.1016/j.celrep.2018.09.006. UCSF: Retrieved from: http://www.escholarship.org/uc/item/59x5k18x
Cell Reports, Vol 25, Iss 4, Pp 871-883 (2018)
Cell Reports, vol 25, iss 4
Cheng, J B, Sedgewick, A J, Finnegan, A I, Harirchian, P, Lee, J, Kwon, S, Fassett, M S, Golovato, J, Gray, M, Ghadially, R, Liao, W, Perez White, B E, Mauro, T M, Mully, T, Kim, E A, Sbitany, H, Neuhaus, I M, Grekin, R C, Yu, S S, Gray, J W, Purdom, E, Paus, R, Vaske, C J, Benz, S C, Song, J S & Cho, R J 2018, ' Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution ', Cell Reports, vol. 25, no. 4, pp. 871-883 . https://doi.org/10.1016/j.celrep.2018.09.006

Summary: Perturbations in the transcriptional programs specifying epidermal differentiation cause diverse skin pathologies ranging from impaired barrier function to inflammatory skin disease. However, the global scope and organization of this complex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47fee77084a0c815c7507f98fa0c06d8
https://doi.org/10.1016/j.celrep.2018.09.006