Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Pawel Jajesniak"'
Autor:
Weronika Witkowska McConnell, Chris Davis, Suleman R. Sabir, Alice Garrett, Amanda Bradley-Stewart, Pawel Jajesniak, Julien Reboud, Gaolian Xu, Zhugen Yang, Rory Gunson, Emma C. Thomson, Jonathan M. Cooper
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Current HCV nucleic acid-based diagnosis is largely performed in centralised laboratories. Here, the authors present a pan-genotypic RNA assay, based on reverse transcriptase loop mediated isothermal amplification and develop a low-cost prototype pap
Externí odkaz:
https://doaj.org/article/70d48ed159ba44c5ab33a8ce3a115976
Autor:
Pawel Jajesniak, Tuck Seng Wong
Publikováno v:
AIMS Bioengineering, Vol 2, Iss 3, Pp 277-296 (2015)
Since the advent of genetic engineering, Escherichia coli, the most widely studied prokaryotic model organism, and other bacterial species have remained at the forefront of biological research. These ubiquitous microorganisms play an essential role i
Externí odkaz:
https://doaj.org/article/98bd89bd652448fc8059b3520e6a3856
Publikováno v:
International Journal of Molecular Sciences, Vol 20, Iss 16, p 3908 (2019)
QuickStep is a cloning method that allows seamless point integration of a DNA sequence at any position within a target plasmid using only Q5 High-Fidelity DNA Polymerase and DpnI endonuclease. This efficient and cost-effective method consists of two
Externí odkaz:
https://doaj.org/article/16cb3318ccc74d75b0414f29ed147d07
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2461
PTO-QuickStep is a quick and easy molecular cloning technique that allows seamless point integration of a DNA fragment, encoding either a tag or a protein, into any position within a target plasmid. The entire process is conducted in a time-efficient
Publikováno v:
Methods in Molecular Biology ISBN: 9781071621516
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::388fe6871e93a45f9358a93a0a22af0a
https://doi.org/10.1007/978-1-0716-2152-3_8
https://doi.org/10.1007/978-1-0716-2152-3_8
Autor:
Jonathan M. Cooper, Pawel Jajesniak, Alice Garrett, Suleman R. Sabir, Amanda Bradley-Stewart, Julien Reboud, Rory Gunson, Gaolian Xu, Chris Davis, E. Thomson, Weronika Witkowska McConnell, Zhugen Yang
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Nature Communications
Nature Communications
The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b9199ef076ab015d54d92b15f7060f4
https://researchonline.lshtm.ac.uk/id/eprint/4664139/1/s41467-021-27076-z.pdf
https://researchonline.lshtm.ac.uk/id/eprint/4664139/1/s41467-021-27076-z.pdf
Autor:
Tuck Seng Wong, Pawel Jajesniak
Publikováno v:
AIMS Bioengineering, Vol 2, Iss 3, Pp 277-296 (2015)
Since the advent of genetic engineering, Escherichia coli, the most widely studied prokaryotic model organism, and other bacterial species have remained at the forefront of biological research. These ubiquitous microorganisms play an essential role i
Autor:
Pawel, Jajesniak, Tuck Seng, Wong
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1472
QuickStep-Cloning is a novel molecular cloning technique that builds upon the concepts of asymmetric PCR and megaprimer-based amplification of whole plasmid. It was designed specifically to address the major drawbacks of previously reported cloning m
Autor:
Pawel Jajesniak, Tuck Seng Wong
Publikováno v:
Methods in Molecular Biology ISBN: 9781493963416
QuickStep-Cloning is a novel molecular cloning technique that builds upon the concepts of asymmetric PCR and megaprimer-based amplification of whole plasmid. It was designed specifically to address the major drawbacks of previously reported cloning m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::864f367e4bf2f6255fdcda3ee5f430ea
https://doi.org/10.1007/978-1-4939-6343-0_16
https://doi.org/10.1007/978-1-4939-6343-0_16
Autor:
Pawel, Jajesniak, Tuck Seng, Wong
Publikováno v:
Journal of Biological Engineering
Background Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cloning. Their widespread use, h