Removing auto-activators from yeast-two-hybrid assays by conditional negative selection

Autor: Pawel Gluza, Devendra Shivhare, Irene Julca, Marek Mutwil, Magdalena Musialak-Lange

Publikováno v: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)

Yeast-two-hybrid (Y2H) is widely used as a strategy to detect protein–protein interactions (PPIs). Recent advancements have made it possible to generate and analyse genome-wide PPI networks en masse by coupling Y2H with next-generation sequencing t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8f01ccecb58f06aef174a71f8990d79
https://doi.org/10.1038/s41598-021-84608-9

A Pipeline towards the Biochemical Characterization of the

Autor: Lingling, Xuan, Jie, Zhang, Weitai, Lu, Pawel, Gluza, Berit, Ebert, Toshihisa, Kotake, Mengzhu, Lu, Yuan, Zhang, Mads H, Clausen, Kim L, Johnson, Monika S, Doblin, Joshua L, Heazlewood, Antony, Bacic, Lili, Song, Wei, Zeng

Publikováno v: International Journal of Molecular Sciences

Glycosyltransferases (GTs) catalyze the synthesis of glycosidic linkages and are essential in the biosynthesis of glycans, glycoconjugates (glycolipids and glycoproteins), and glycosides. Plant genomes generally encode many more GTs than animal genom

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::45e4305cce9ec076c7fd42b703288f66
https://pubmed.ncbi.nlm.nih.gov/33572987

The Three Members of the Arabidopsis thaliana Glycosyltransferase Family 92 are Functional β-1,4-Galactan Synthases

Autor: Tomas Laursen, April J. M. Liwanag, Michela Catena, Antony Bacic, Emilie A. Rennie, Venkataramana R. Pidatala, William G.T. Willats, Mads Hartvig Clausen, Solomon Stonebloom, Jenny C. Mortimer, Joshua L. Heazlewood, Roshan Cheetamun, Mathias Christian Franch Andersen, Xiaoyuan Guo, Henrik Vibe Scheller, Carsten Rautengarten, Devon Birdseye, Berit Ebert, Pawel Gluza

Publikováno v: Ebert, B; Birdseye, D; Liwanag, AJM; Laursen, T; Rennie, EA; Guo, X; et al.(2018). The Three Members of the Arabidopsis thaliana Glycosyltransferase Family 92 are Functional β-1,4-Galactan Synthases.. Plant & cell physiology. doi: 10.1093/pcp/pcy180. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/5v02w456
Ebert, B, Birdseye, D, Liwanag, A J M, Laursen, T, Rennie, E A, Guo, X, Catena, M, Rautengarten, C, Stonebloom, S H, Gluza, P, Pidatala, V, Andersen, M C F, Cheetamun, R, Mortimer, J C, Heazlewood, J L, Bacic, A, Clausen, M H, Willats, W G T & Scheller, H V 2018, ' The Three Members of the Arabidopsis thaliana Glycosyltransferase Family 92 are Functional β-1,4-Galactan Synthases ', Plant and Cell Physiology, vol. 59, no. 12, pp. 2624-2636 . https://doi.org/10.1093/pcp/pcy180

Pectin is a major component of primary cell walls and performs a plethora of functions crucial for plant growth, development and plant-defense responses. Despite the importance of pectic polysaccharides their biosynthesis is poorly understood. Severa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed20c521b9e4cf3aad574220143d5701
http://www.escholarship.org/uc/item/5v02w456