Application of FRET- and BRET-based live-cell biosensors in deorphanization and ligand discovery studies on orphan G protein-coupled receptors

Autor: Joanna J. Sajkowska, Choi Har Tsang, Paweł Kozielewicz

Publikováno v: SLAS Discovery, Vol 29, Iss 6, Pp 100174- (2024)

Bioluminescence- and fluorescence-based resonance energy transfer assays have gained considerable attention in pharmacological research as high-throughput scalable tools applicable to drug discovery. To this end, G protein-coupled receptors represent

Externí odkaz: https://doaj.org/article/14dcede2a25b44e698c22c77b0bfd532

A conserved molecular switch in Class F receptors regulates receptor activation and pathway selection

Autor: Shane C. Wright, Paweł Kozielewicz, Maria Kowalski-Jahn, Julian Petersen, Carl-Fredrik Bowin, Greg Slodkowicz, Maria Marti-Solano, David Rodríguez, Belma Hot, Najeah Okashah, Katerina Strakova, Jana Valnohova, M. Madan Babu, Nevin A. Lambert, Jens Carlsson, Gunnar Schulte

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)

Class F receptors are therapeutic targets in human disease and understanding their structural changes during receptor activation may provide important pharmacological insight. Here, the authors combine computational and experimental methods to identi

Externí odkaz: https://doaj.org/article/25eb2e5a80d940629421bb3eaf96fb01

NanoBiT ‐ and NanoBiT/BRET ‐based assays allow the analysis of binding kinetics of Wnt‐3a to endogenous Frizzled 7 in a colorectal cancer model

Autor: Lukas Grätz, Joanna J. Sajkowska‐Kozielewicz, Janine Wesslowski, Julia Kinsolving, Lloyd J. Bridge, Katja Petzold, Gary Davidson, Gunnar Schulte, Paweł Kozielewicz

Publikováno v: British Journal of Pharmacology.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f70eaae65ff7db1f116722e8c329f2f3
https://doi.org/10.1111/bph.16090

Receptor levels determine binding affinity of WNT-3A to Frizzled 7 in a colorectal cancer model

Autor: Lukas Grätz, Joanna J. Sajkowska-Kozielewicz, Janine Wesslowski, Katja Petzold, Gary Davidson, Gunnar Schulte, Paweł Kozielewicz

WNT binding to Frizzleds (FZD) is a crucial step that leads to the initiation of signalling cascades governing multiple processes during embryonic development, stem cell regulation and adult tissue homeostasis. Recent efforts have enabled us to shed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::53a81c38a4610d92f949ea217da52f5f
https://doi.org/10.1101/2022.07.04.498383

The Concise Guide To Pharmacology 2021/22: G Protein-Coupled Receptors

Autor: Nigel J. M. Birdsall, Stephen J Lolait, Hans-Jürgen Wester, Paul L. Chazot, Sadashiva S. Karnik, Simon D. Harding, Celine Valant, Stephen P.H. Alexander, Olivier Civelli, Zsolt Csaba, Mastgugu Horiuchi, Khuraijam Dhanachandra Singh, Theodore L. Goodfriend, Morley D. Hollenberg, Duuamene Nyimanu, Shlomo Melmed, G. Enrico Rovati, Xavier Norel, Leigh A. Stoddart, Klemens Kaupmann, Robyn Macrae, Nicholas D. Holliday, Deborah L. Segaloff, Justo P. Castaño, Tony Ngo, Gordon Dent, Jean-Martin Beaulieu, Thomas L Williams, Antonia Cianciulli, Paweł Kozielewicz, Xaria X. Li, Jyrki P. Kukkonen, Craig A. McArdle, John D. Lee, Philippe Rondard, Steven D. Douglas, Hubert Vaudry, Khaled A. Al-Hosaini, Nan Chiang, Bernhard Bettler, Giovanni Tulipano, Corinne Bousquet, Karen J. Gregory, Craig Gerard, Robert T. Jensen, Stefan Schulz, Rithwik Ramachandran, Ross A. D. Bathgate, Deepa Jonnalagadda, David M. Thal, Takio Kitazawa, Manisha Ray, Bice Chini, Thomas Unger, Marta Fumagalli, Jean-Pierre Vilardaga, Donald T. Ward, Roger G. Pertwee, Stefan Offermanns, Marvin C. Gershengorn, Marc Parmentier, Pieter Timmermans, Eamonn Kelly, Yukihiko Sugimoto, Hamiyet Unal, Vincenzo Mitolo, Alistair Mathie, Emma L. Veale, Andrew L. Gundlach, Anthony P. Davenport, Mark T. Quinn, Jérôme Leprince, Christa E. Müller, Geoffrey Burnstock, Akos Heinemann, Jacqueline R. Kemp, Richard D. Ye, Bernard Mouillac, Roger J. Summers, Raul R. Gainetdinov, Girolamo Calò, Philip N. Murphy, Amelie Lupp, Kalyan C. Tirupula, Walter G. Thomas, Julien Hanson, Ahsan Husain, Katie Leach, Lucie H. Clapp, Hans Bräuner-Osborne, Gunnar Schulte, Kenneth E. Bernstein, Jean Mazella, Torsten Schöneberg, Satoru Eguchi, Martin C. Michel, Maria Antonietta Panaro, Kevin J. Catt, Rainer K. Reinscheid, Hans-Jürgen Kreienkamp, Wayne R. Alexander, Emanuel Escher, Anne Marie O'Carroll, Magnus Bäck, Laurence J. Miller, Jane F. Armstrong, Chiara Ruzza, Trent M. Woodruff, Daniel Hoyer, Chengcan Yao, Maria P. Abbracchio, John A. Peters, Gary B. Willars, Jean-Philippe Pin, David Vaudry, Debbie L. Hay, François Boulay, Davide Lecca, Eric R. Prossnitz, Arthur Christopoulos, Victoria A. Blaho, Yasuyuki Kihara, Charles Kennedy, Christopher Southan, László Hunyady, Pascal Dournaud, Fernand Gobeil, Cyril Goudet, Charles N. Serhan, Claes Dahlgren, Jörg Hamann, Tobias Langenhan, Ralf Jockers, Nicholas M. Barnes, Jean-Louis Nahon, Richard L. Hauger, Adam J. Pawson, Gareth J. Sanger, Tung Fong, Susan E. Leeman, Elena Faccenda, Edward J. Filardo, Valerie P. Tan, Marc de Gasparo, Ji Ming Wang, Jamie A. Davies, Jerold Chun, Stefania Ceruti, Tadashi Inagami, Réjean Couture, Kenneth A. Jacobson, Patrick Vanderheyden, Adriaan P. IJzerman, Janet J. Maguire, Christopher S. Walker

Publikováno v: British Journal of Pharmacology, 178, S27-S156. Wiley
Alexander, S P, Christopoulos, A, Davenport, A P, Kelly, E, Mathie, A, Peters, J A, Veale, E L, Armstrong, J F, Faccenda, E, Harding, S D, Pawson, A J, Southan, C, Davies, J A, Abbracchio, M P, Alexander, W, Al-Hosaini, K, Bäck, M, Barnes, N M, Bathgate, R, Beaulieu, J-M, Bernstein, K E, Bettler, B, Birdsall, N J M, Blaho, V, Boulay, F, Bousquet, C, Bräuner-Osborne, H, Burnstock, G, Caló, G, Castaño, J P, Catt, K J, Ceruti, S, Chazot, P, Chiang, N, Chini, B, Chun, J, Cianciulli, A, Civelli, O, Clapp, L H, Couture, R, Csaba, Z, Dahlgren, C, Dent, G, Singh, K D, Douglas, S D, Dournaud, P, Eguchi, S, Escher, E, Filardo, E J, Fong, T, Fumagalli, M, Gainetdinov, R R, Gasparo, M D, Gerard, C, Gershengorn, M, Gobeil, F, Goodfriend, T L, Goudet, C, Gregory, K J, Gundlach, A L, Hamann, J, Hanson, J, Hauger, R L, Hay, D L, Heinemann, A, Hollenberg, M D, Holliday, N D, Horiuchi, M, Hoyer, D, Hunyady, L, Husain, A, IJzerman, A P, Inagami, T, Jacobson, K A, Jensen, R T, Jockers, R, Jonnalagadda, D, Karnik, S, Kaupmann, K, Kemp, J, Kennedy, C, Kihara, Y, Kitazawa, T, Kozielewicz, P, Kreienkamp, H-J, Kukkonen, J P, Langenhan, T, Leach, K, Lecca, D, Lee, J D, Leeman, S E, Leprince, J, Li, X X, Williams, T L, Lolait, S J, Lupp, A, Macrae, R, Maguire, J, Mazella, J, McArdle, C A, Melmed, S, Michel, M C, Miller, L J, Mitolo, V, Mouillac, B, Müller, C E, Murphy, P, Nahon, J-L, Ngo, T, Norel, X, Nyimanu, D, O'Carroll, A-M, Offermanns, S, Panaro, M A, Parmentier, M, Pertwee, R G, Pin, J-P, Prossnitz, E R, Quinn, M, Ramachandran, R, Ray, M, Reinscheid, R K, Rondard, P, Rovati, G E, Ruzza, C, Sanger, G J, Schöneberg, T, Schulte, G, Schulz, S, Segaloff, D L, Serhan, C N, Stoddart, L A, Sugimoto, Y, Summers, R, Tan, V P, Thal, D, Thomas, W W, Timmermans, P B M W M, Tirupula, K, Tulipano, G, Unal, H, Unger, T, Valant, C, Vanderheyden, P, Vaudry, D, Vaudry, H, Vilardaga, J-P, Walker, C S, Wang, J M, Ward, D T, Wester, H-J, Willars, G B, Woodruff, T M, Yao, C & Ye, R D 2021, ' THE CONCISE GUIDE TO PHARMACOLOGY 2021/22 : G protein-coupled receptors ', British Journal of Pharmacology, vol. 178 Suppl 1, no. S1, pp. S27-S156 . https://doi.org/10.1111/bph.15538
British journal of pharmacology, 178(S1), S27-S156. Wiley-Blackwell
Alexander, S P, Christopoulos, A, Davenport, A P, Kelly, E, Mathie, A, Peters, J A, Veale, E L, Armstrong, J F, Faccenda, E, Harding, S D, Pawson, A J, Southan, C, Davies, J A, Abbracchio, M P, Alexander, W, Al-Hosaini, K, Bäck, M, Barnes, N M, Bathgate, R, Beaulieu, J-M, Bernstein, K E, Bettler, B, Birdsall, N J M, Blaho, V, Boulay, F, Bousquet, C, Bräuner-Osborne, H, Burnstock, G, Caló, G, Castaño, J P, Catt, K J, Ceruti, S, Chazot, P, Chiang, N, Chini, B, Chun, J, Cianciulli, A, Civelli, O, Clapp, L H, Couture, R, Csaba, Z, Dahlgren, C, Dent, G, Singh, K D, Douglas, S D, Dournaud, P, Eguchi, S, Escher, E, Filardo, E J, Fong, T, Fumagalli, M, Gainetdinov, R R, Gasparo, M D, Gerard, C, Gershengorn, M, Gobeil, F, Goodfriend, T L, Goudet, C, Gregory, K J, Gundlach, A L, Hamann, J, Hanson, J, Hauger, R L, Hay, D L, Heinemann, A, Hollenberg, M D, Holliday, N D, Horiuchi, M, Hoyer, D, Hunyady, L, Husain, A, IJzerman, A P, Inagami, T, Jacobson, K A, Jensen, R T, Jockers, R, Jonnalagadda, D, Karnik, S, Kaupmann, K, Kemp, J, Kennedy, C, Kihara, Y, Kitazawa, T, Kozielewicz, P, Kreienkamp, H-J, Kukkonen, J P, Langenhan, T, Leach, K, Lecca, D, Lee, J D, Leeman, S E, Leprince, J, Li, X X, Williams, T L, Lolait, S J, Lupp, A, Macrae, R, Maguire, J, Mazella, J, McArdle, C A, Melmed, S, Michel, M C, Miller, L J, Mitolo, V, Mouillac, B, Müller, C E, Murphy, P, Nahon, J-L, Ngo, T, Norel, X, Nyimanu, D, O'Carroll, A-M, Offermanns, S, Panaro, M A, Parmentier, M, Pertwee, R G, Pin, J-P, Prossnitz, E R, Quinn, M, Ramachandran, R, Ray, M, Reinscheid, R K, Rondard, P, Rovati, G E, Ruzza, C, Sanger, G J, Schöneberg, T, Schulte, G, Schulz, S, Segaloff, D L, Serhan, C N, Stoddart, L A, Sugimoto, Y, Summers, R, Tan, V P, Thal, D, Thomas, W W, Timmermans, P B M W M, Tirupula, K, Tulipano, G, Unal, H, Unger, T, Valant, C, Vanderheyden, P, Vaudry, D, Vaudry, H, Vilardaga, J-P, Walker, C S, Wang, J M, Ward, D T, Wester, H-J, Willars, G B, Woodruff, T M, Yao, C & Ye, R D 2021, ' THE CONCISE GUIDE TO PHARMACOLOGY 2021/22 : G protein-coupled receptors ', British Journal of Pharmacology, vol. 178, no. S1, pp. S27-S156 . https://doi.org/10.1111/bph.15538

The Concise Guide to PHARMACOLOGY 2021/22 is the fifth in this series of biennial publications. The Concise Guide provides concise overviews, mostly in tabular format, of the key properties of nearly 1900 human drug targets with an emphasis on select

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eef5f3d1320e3d9fb87604fe0e7987c5
https://cris.maastrichtuniversity.nl/en/publications/e6053af4-7c66-44a3-97bc-f7b87d1745a9