Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Pavel V. Natashin"'
Autor:
Pavel V. Natashin, Elena V. Eremeeva, Mikhail B. Shevtsov, Margarita I. Kovaleva, Sergey S. Bukhdruker, Daria A. Dmitrieva, Dmitry V. Gulnov, Elena V. Nemtseva, Valentin I. Gordeliy, Alexey V. Mishin, Valentin I. Borshchevskiy, Eugene S. Vysotski
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-14 (2022)
Abstract Coelenterazine-v (CTZ-v), a synthetic vinylene-bridged π-extended derivative, is able to significantly alter bioluminescence spectra of different CTZ-dependent luciferases and photoproteins by shifting them towards longer wavelengths. Howev
Externí odkaz:
https://doaj.org/article/5e569ca5c75d43e5939327cb2a853179
Autor:
Pavel V. Natashin, Ludmila P. Burakova, Margarita I. Kovaleva, Mikhail B. Shevtsov, Daria A. Dmitrieva, Elena V. Eremeeva, Svetlana V. Markova, Alexey V. Mishin, Valentin I. Borshchevskiy, Eugene S. Vysotski
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 7, p 6869 (2023)
Hydromedusan photoproteins responsible for the bioluminescence of a variety of marine jellyfish and hydroids are a unique biochemical system recognized as a stable enzyme-substrate complex consisting of apoprotein and preoxygenated coelenterazine, wh
Externí odkaz:
https://doaj.org/article/11e073fe86c84d68ba9798095d78ff2e
Autor:
Natalia P. Malikova, Dmitry V. Gulnov, Elena V. Nemtseva, Eugene S. Vysotski, Elena V. Eremeeva, Pavel V. Natashin
Publikováno v:
Photochemistry and Photobiology. 98:276-284
Nowadays the recombinant Ca2+ -regulated photoproteins originating from marine luminous organisms are widely applied to monitor calcium transients in living cells due to their ability to emit light on Ca2+ binding. Here we report the specific activit
Autor:
Lijie Wu, Dmitry V. Gulnov, Elena V. Nemtseva, Elena V. Eremeeva, Marina D. Larionova, Pavel V. Natashin, Zhi-Jie Liu, Dongsheng Liu, Eugene S. Vysotski
Publikováno v:
Protein Sci
Coelenterazine‐v (CTZ‐v), a synthetic derivative with an additional benzyl ring, yields a bright bioluminescence of Renilla luciferase and its “yellow” mutant with a significant shift in the emission spectrum toward longer wavelengths, which
Autor:
Pavel V. Natashin, Natalia P. Malikova, Elena V. Eremeeva, Svetlana V. Markova, Zhi-Jie Liu, Chongyun Cheng, Ludmila P. Burakova, Eugene S. Vysotski
Publikováno v:
Journal of Photochemistry and Photobiology B: Biology. 162:286-297
The full-length cDNA genes encoding five new isoforms of Ca2 +-regulated photoprotein mitrocomin from a small tissue sample of the outer bell margin containing photocytes of only one specimen of the luminous jellyfish Mitrocoma cellularia were cloned
Autor:
Eugene S. Vysotski, Pavel V. Natashin, Zhi-Jie Liu, Natalia P. Malikova, Mengchen Pu, Fengfeng Niu, Ludmila Burakova
Publikováno v:
Journal of Photochemistry and Photobiology B: Biology. 154:57-66
Light-sensitive photoprotein berovin accounts for a bright bioluminescence of ctenophore Beroe abyssicola. Berovin is functionally identical to the well-studied Ca(2+)-regulated photoproteins of jellyfish, however in contrast to those it is extremely
Publikováno v:
The FEBS Journal. 281:1432-1445
Ca(2+) -regulated photoproteins are responsible for the bioluminescence of a variety of marine coelenterates. All hydromedusan photoproteins are a single-chain polypeptide to which 2-hydroperoxycoelenterazine is tightly but non-covalently bound. Biol
Autor:
Eugene S. Vysotski, Pavel V. Natashin, John Lee, Wei Ding, Svetlana V. Markova, Elena V. Eremeeva, Zhi-Jie Liu
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 70(Pt 3)
Ca2+-regulated photoproteins, which are responsible for light emission in a variety of marine coelenterates, are a highly valuable tool for measuring Ca2+inside living cells. All of the photoproteins are a single-chain polypeptide to which a 2-hydrop
Autor:
Yuguang Zhou, Lei Song, Elena V. Eremeeva, Eugene S. Vysotski, Zhi-Jie Liu, Pavel V. Natashin, Willem J. H. van Berkel
Publikováno v:
ChemBioChem 14 (2013) 6
ChemBioChem, 14(6), 739-745
ChemBioChem, 14(6), 739-745
Ca(2+) -regulated photoproteins use a noncovalently bound 2-hydroperoxycoelenterazine ligand to emit light in response to Ca(2+) binding. To better understand the mechanism of formation of active photoprotein from apoprotein, coelenterazine and molec