Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Pavel A. Zhulidov"'
Autor:
A. S. Shcheglov, Sergey Lukyanov, Pavel A. Zhulidov, Denis V. Rebrikov, Veronika E. Anisimova, Dmitry B. Staroverov
Publikováno v:
Biochemistry (Moscow). 71:513-519
We overexpressed duplex-specific nuclease (DSN) from Kamchatka crab in Escherichia coli cells and developed procedures for purification, renaturation, and activation of this protein. We demonstrated identity of the properties of the native and recomb
Publikováno v:
Russian Journal of Bioorganic Chemistry. 31:567-575
A new modification of the single nucleotide polymorphism (SNP) analysis (DSNP, duplex-specific nuclease preference) method using the duplex-specific nuclease from the king crab was proposed. The method was used to study SNPs in the following human ge
Autor:
Dmitry A. Shagin, Valery B. Kozhemyako, Ekaterina A. Bogdanova, Alex S. Shcheglov, Ilia M. Altshuler, Sergey Lukyanov, Denis V. Rebrikov, Pavel A. Zhulidov, Dmitry B. Staroverov, Valery A. Rasskazov
Publikováno v:
Genome Research. 12:1935-1942
We have characterized a novel nuclease from the Kamchatka crab, designated duplex-specific nuclease (DSN). DSN displays a strong preference for cleaving double-stranded DNA and DNA in DNA-RNA hybrid duplexes, compared to single-stranded DNA. Moreover
Publikováno v:
Nucleic Acids Hybridization Modern Applications ISBN: 9781402060397
In a cellular transcriptome, the number of mRNA copies per gene may differ by several orders. In cDNA libraries, performed from mRNA, these proportions are the same. Normalization methods allow us to equalize numbers of gene’s copies in the library
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c002b45eb25718dbce39db9be32d4341
https://doi.org/10.1007/978-1-4020-6040-3_5
https://doi.org/10.1007/978-1-4020-6040-3_5
Publikováno v:
European Journal of Cancer Supplements. 4(6)
Autor:
Alex S. Shcheglov, Ekaterina A. Bogdanova, Valery B. Kozhemyako, Sergey Lukyanov, George L. Khaspekov, Leonid L. Moroz, Ella A. Meleshkevitch, Mikhail V. Matz, Vagner Ll, Dmitry A. Shagin, Pavel A. Zhulidov
Publikováno v:
Nucleic Acids Research. 32:37e-37
We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation-reassociation o
Publikováno v:
European Journal of Cancer Supplements. 2:41