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pro vyhledávání: '"Paula Sebastiao"'
Autor:
Anastassis Perrakis, George S. Brush, Paula Sebastiao, Keith S. Wilson, G. Obmolova, Alexei Teplyakov, Maurice J. Bessman
Publikováno v:
The EMBO Journal. 15:3487-3497
NMP kinases catalyse the phosphorylation of the canonical nucleotides to the corresponding diphosphates using ATP as a phosphate donor. Bacteriophage T4 deoxynucleotide kinase (DNK) is the only member of this family of enzymes that recognizes three s
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 52(Pt 1)
T4 deoxynucleotide kinase catalyzes the phosphorylation of 5-hydroxymethyldeoxycytidylate, dTMP and dGMP while excluding dCMP and dAMP. In order to understand the mechanism of this remarkable specificity, the enzyme was over-expressed in Escherichia