Výsledky vyhledávání - "Paula D. Ladd"

A low-cost and easy-to-use cell preservation reagent for 4°C or room temperature sample storage

Autor: Ari Geary-Teeter, Sidharth Shankar, Shreya Roy, George M. Martin, Paula D. Ladd

Current biospecimen storage and preservation methods no longer meet the demands of basic research and clinical diagnostics. Biospecimen preservation methodology has not advanced to accommodate cutting edge molecular analysis technologies that target

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba319594113a6fcff40f6686c6fe195f

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WRNMutation Update: Mutation Spectrum, Patient Registries, and Translational Prospects

Publikováno v: Human Mutation. 38:7-15

Werner syndrome (WS) is a rare autosomal recessive disorder characterized by a constellation of adult onset phenotypes consistent with an acceleration of intrinsic biological aging. It is caused by pathogenic variants in the WRN gene, which encodes a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::501b5fcb18aaba8a77cbc4cafdf169b4
https://doi.org/10.1002/humu.23128

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Comparison of point-of-care-compatible lysis methods for bacteria and viruses

Autor: Paula D. Ladd, Barry R. Lutz, Erin K. Heiniger, Joshua R. Buser, Paul Yager, Xiaohong Zhang, Lillian Mireles

Publikováno v: Journal of Microbiological Methods. 128:80-87

Nucleic acid sample preparation has been an especially challenging barrier to point-of-care nucleic acid amplification tests in low-resource settings. Here we provide a head-to-head comparison of methods for lysis of, and nucleic acid release from, s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c591439f11b7fa7ba7cba704e41ad53
https://doi.org/10.1016/j.mimet.2016.07.007

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A disposable chemical heater and dry enzyme preparation for lysis and extraction of DNA and RNA from microorganisms

Autor: Maxwell Wheeler, Paul Yager, Bernhard H. Weigl, Paula D. Ladd, Joshua R. Buser, Janet A. Englund, Xiaohong Zhang, Samantha A. Byrnes, Barry R. Lutz, Erin K. Heiniger, Joshua D. Bishop

Publikováno v: Analytical Methods. 8:2880-2886

Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::74e3f337a66fa9b97b2d0d7f56dbdef4
https://doi.org/10.1039/c6ay00107f

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A rapid, instrument-free, sample-to-result nucleic acid amplification test

Autor: Sujatha Kumar, Lisa Lafleur, Joshua D. Bishop, Xiaohong Zhang, Enos Kline, Peter Kauffman, Barry R. Lutz, Nicolaas M. J. Vermeulen, Ryan Gallagher, Paul Yager, Bhushan J. Toley, Joshua R. Buser, Paula D. Ladd, Noah Scarr, Samantha A. Byrnes, Erin K. Heiniger, Walt Mahoney, Yevgeniy S. Belousov, Maxwell Wheeler

Publikováno v: Lab on a Chip. 16:3777-3787

The prototype demonstrated here is the first fully integrated sample-to-result diagnostic platform for performing nucleic acid amplification tests that requires no permanent instrument or manual sample processing. The multiplexable autonomous disposa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d9d84b985228e821678593d064d76c8
https://doi.org/10.1039/c6lc00677a

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Rapid sample preparation with Lyse-It® for Listeria monocytogenes and Vibrio cholerae

Autor: Tonya M. Santaus, Paula D. Ladd, O. Colin Stine, Shan Li, Chris D. Geddes, Shannon Cole, Amanda Harvey

Publikováno v: PLoS ONE, Vol 13, Iss 7, p e0201070 (2018)
PLoS ONE

Sample preparation is a leading bottleneck in rapid detection of pathogenic bacteria. Here, we use Lyse-It® for bacterial cellular lysis, genomic DNA fragmentation, and protein release and degradation for both Listeria monocytogenes and Vibrio chole

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c519200d8c7c83b84a181e3b5903b8e7
http://europepmc.org/articles/PMC6059484?pdf=render

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Electromechanical cell lysis using a portable audio device: enabling challenging sample preparation at the point-of-care

Autor: Peter Kauffman, Joshua R. Buser, Alec Wollen, Samantha A. Byrnes, Erin K. Heiniger, Paul Yager, Paula D. Ladd

Publikováno v: Lab on a Chip. 15:1994-1997

Audio sources are ubiquitously available on portable electronic devices, including cell phones. Here we demonstrate lysis of Mycobacterium marinum and Staphylococcus epidermidis bacteria utilizing a portable audio device coupled with a simple and ine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::497ec0575f70821a370ee5225092b219
https://doi.org/10.1039/c5lc00080g

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Lyse-It™: A Rapid Platform for Cellular Lysing and Tunable DNA/Protein Fragmentation

Autor: Maraki Y. Negesse, Melissa Cyr, Johan H. Melendez, Tonya M. Santaus, Amanda Harvey, Paula D. Ladd, Chris D. Geddes

Publikováno v: Microwave Effects on DNA and Proteins ISBN: 9783319502885

In this article, we review Lyse-It™; a method for the low cost, efficient, and rapid sample preparation of various bacteria and cell types. Currently, bacterial detection and identification is underpinned by two discreet processes, namely genomic i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d1b0487fd0bd5bf7607543a332a29cea
https://doi.org/10.1007/978-3-319-50289-2_8

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