Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Paul W. Hastwell"'
The BlueScreen-384 Assay as an Indicator of Genotoxic Hazard Potential in Early-Stage Drug Discovery
Autor:
Poonam Shah, Paul W. Hastwell, Andrew J. Brown, Steve Rees, Kate Simpson, Elke Gogo, Nicola Bevan, Patrick M. Eidam
Publikováno v:
SLAS Discovery. 18:441-452
High-throughput cell-based techniques that permit early detection of compound-induced genotoxic damage have recently become available. Methods based on induction of the GADD45a promoter are attractive because multiple intracellular mechanisms that de
Autor:
Anne Marie Vinggaard, Christopher Jagger, Julia Rytter Hansen, Adam Rabinowitz, Kamala Pant, Robert W. Rees, Nicholas Billinton, Matthew Tate, Richard M. Walmsley, Shannon W. Bruce, Paul W. Hastwell, Michelle L. Klug, Catriona McComb
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 700:44-50
A new protocol has recently been developed and validated for the GreenScreen HC GADD45a-GFP genotoxicity reporter assay, enabling the incorporation of an S9 metabolic activation system into the assay. The S9 protocol employs flow-cytometric methodolo
Autor:
James M. Parry, Paul W. Hastwell, Jean-Roc Meunier, Véronique Thybaud, Daniel Marzin, Lutz Müller, Firouz Darroudi, Anthony M. Lynch, Marilyn J. Aardema, Paul D. White, David Tweats, Azeddine Elhajouji, Gerhard J. Nohynek, Ray Tice, Daniela Maurici, Peter Kasper, M. Hayashi, Stefan Pfuhler, Stephan Kirchner, Raffaella Corvi, Elizabeth M. Parry, Philippe Vanparys, David Kirkland, Jan van Benthem, Hansruedi Glatt
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 628:31-55
Workshop participants agreed that genotoxicity tests in mammalian cells in vitro produce a remarkably high and unacceptable occurrence of irrelevant positive results (e.g. when compared with rodent carcinogenicity). As reported in several recent revi
Publikováno v:
Mutagenesis. 28(4)
Expression of the human GADD45a gene is increased in TK6 cells exposed to mutagens, clastogens and aneugens. It is known to be regulated through both p53-dependent and p53-independent pathways and WT1 has been implicated in both cases. This article r
Autor:
Paul W. Hastwell, James Harvey, Thomas W. Webster, Robert W. Rees, Matthew Tate, Nicholas Billinton, Anthony M. Lynch, Richard M. Walmsley
Publikováno v:
Mutagenesis. 24(5)
The GADD45a-GFP (GreenScreen HC) reporter assay detects genotoxic damage in the human lymphoblastoid TK6 cell line and gives positive results for all classes of genotoxin, including mutagens, aneugens and clastogens. In this study, a collection of 75
Autor:
Nicholas Billinton, Thomas W. Webster, David Tweats, Louise Birrell, Filip Woestenborghs, Paul W. Hastwell, Robert W. Rees, Sharon Maskell, Richard M. Walmsley, Andrew D. Scott, Jacky Van Gompel, Anthony M. Lynch, Sam Windebank, Patricia Ellis, Dominiek Beerens
Publikováno v:
Mutation research. 653(1-2)
Sixteen coded compounds were blind-tested at 4 laboratories using the recently described GADD45a-GFP genotoxicity assay. The compounds were chosen to include non-genotoxic compounds as well as weak and strong genotoxins. None of the compounds require
Autor:
James Harvey, Thomas W. Webster, Paul W. Hastwell, Robert W. Rees, Kevin J. Roberts, Richard M. Walmsley, Li Leng Chai
Publikováno v:
Mutation research. 607(2)
The battery of genetic toxicity tests required by most regulatory authorities includes both bacterial and mammalian cell assays and identifies practically all genotoxic carcinogens. However, the relatively high specificity of the Salmonella mutagenic
Autor:
Paul W. Hastwell, Patrick Keenan, Andrew W. Knight, L Walsh, Richard M. Walmsley, Nicholas Billinton
Publikováno v:
Mutagenesis. 20(5)
The yeast (Saccharomyces cerevisiae) RAD54-GFP DNA repair reporter assay (GreenScreen assay, GSA) can be used for early genotoxicity screening in drug discovery. During the initial validation of this preregulatory assay, a subset of known genotoxic c