Zobrazeno 1 - 10
of 171
pro vyhledávání: '"Paul W Buehler"'
Autor:
Mitchell R H Weigand, Jenifer Gómez-Pastora, James Kim, Matthew T Kurek, Richard J Hickey, David C Irwin, Paul W Buehler, Maciej Zborowski, Andre F Palmer, Jeffrey J Chalmers
Publikováno v:
PLoS ONE, Vol 16, Iss 9, p e0257061 (2021)
A new method for hemoglobin (Hb) deoxygenation, in suspension or within red blood cells (RBCs) is described using the commercial enzyme product, EC-Oxyrase®. The enzymatic deoxygenation method has several advantages over established deoxygenation me
Externí odkaz:
https://doaj.org/article/43431974dcc5428b83f6d54eb72192ce
Autor:
Donald A Belcher, Julia A Ju, Jin Hyen Baek, Ayla Yalamanoglu, Paul W Buehler, Daniele M Gilkes, Andre F Palmer
Publikováno v:
PLoS ONE, Vol 13, Iss 2, p e0191275 (2018)
A major constraint in the treatment of cancer is inadequate oxygenation of the tumor mass, which can reduce chemotherapeutic efficacy. We hypothesize that polymerized human hemoglobin (PolyhHb) can be transfused into the systemic circulation to incre
Externí odkaz:
https://doaj.org/article/db402f0e3f0b4bf1b7da163fb38606ff
Autor:
Zoe Loomis, Paul Eigenberger, Katherine Redinius, Christina Lisk, Vijaya Karoor, Eva Nozik-Grayck, Scott K Ferguson, Kathryn Hassell, Rachelle Nuss, Kurt R Stenmark, Paul W Buehler, David C Irwin
Publikováno v:
PLoS ONE, Vol 12, Iss 3, p e0173652 (2017)
[This corrects the article DOI: 10.1371/journal.pone.0171219.].
Externí odkaz:
https://doaj.org/article/159f4039c667483dabd5f002a1d2160d
Publikováno v:
Frontiers in Physiology, Vol 5 (2014)
Preclinical studies suggest that haptoglobin (Hp) supplementation could be an effective therapeutic modality during acute or chronic hemolytic diseases. Hp prevents Hb extravasation and neutralizes Hb’s oxidative and NO scavenging activity in the v
Externí odkaz:
https://doaj.org/article/46687a558cbf434ca801158e777eba34
Publikováno v:
PLoS ONE, Vol 8, Iss 3, p e59841 (2013)
Human hemoglobin (Hb) and haptoglobin (Hp) exhibit an extremely high affinity for each other, and the dissociation of Hb tetramers into dimers is generally believed to be a prerequisite for complex formation. We have investigated Hp interactions with
Externí odkaz:
https://doaj.org/article/8c83054bf6324cc493f7d9d0c90c3b62
Autor:
Gurneet S. Sangha, Callie M. Weber, Ryan M. Sapp, Saini Setua, Kiruphagaran Thangaraju, Morgan Pettebone, Stephen C. Rogers, Allan Doctor, Paul W. Buehler, Alisa M. Clyne
Publikováno v:
Frontiers in Physiology, Vol 14 (2023)
Introduction: Generating physiologically relevant red blood cell extracellular vesicles (RBC-EVs) for mechanistic studies is challenging. Herein, we investigated how to generate and isolate high concentrations of RBC-EVs in vitro via shear stress and
Externí odkaz:
https://doaj.org/article/ee4b96be5fa1485fbb67ad70e659724a
Autor:
Kiruphagaran Thangaraju PhD, Saini Setua PhD, Christina Lisk PhD, Delaney Swindle MS, Daniel Stephenson PhD, Monika Dzieciatkowska PhD, Derek R. Lamb BS, Parikshit Moitra PhD, David Pak MS, Kathryn Hassell MD, Gemlyn George MD, Rachelle Nuss MD, Pavel Davizon-Castillo MD, Kurt R. Stenmark MD, Angelo D’Alessandro PhD, David C. Irwin PhD, Paul W. Buehler PharmD, PhD
Publikováno v:
Clinical and Applied Thrombosis/Hemostasis, Vol 29 (2023)
Aberrant coagulation in sickle cell disease (SCD) is linked to extracellular vesicle (EV) exposure. However, there is no consensus on the contributions of small EVs (SEVs) and large EVs (LEVs) toward underlying coagulopathy or on their molecular carg
Externí odkaz:
https://doaj.org/article/4769d021364e45e397107d74814a04a5
Autor:
Christina Lisk, Francesca Cendali, David I. Pak, Delaney Swindle, Kathryn Hassell, Rachelle Nuss, Gemlyn George, Pavel Davizon-Castillo, Paul W. Buehler, Angelo D’Alessandro, David C. Irwin
Publikováno v:
Frontiers in Medicine, Vol 10 (2023)
IntroductionHuman and murine sickle cell disease (SCD) associated pulmonary hypertension (PH) is defined by hemolysis, nitric oxide depletion, inflammation, and thrombosis. Further, hemoglobin (Hb), heme, and iron accumulation are consistently observ
Externí odkaz:
https://doaj.org/article/2b14caf86c4044b991f1322159efd216
Autor:
Francesca I. Cendali, Travis Nemkov, Christina Lisk, Ian S. Lacroix, Seyed-Mehdi Nouraie, Yingze Zhang, Victor R. Gordeuk, Paul W. Buehler, David Irwin, Angelo D’Alessandro
Publikováno v:
Frontiers in Physiology, Vol 14 (2023)
Introduction: Exercise intolerance is a common clinical manifestation in patients with sickle cell disease (SCD), though the mechanisms are incompletely understood.Methods: Here we leverage a murine mouse model of sickle cell disease, the Berkeley mo
Externí odkaz:
https://doaj.org/article/c879461d9ef3434c9e35c24043888751
Publikováno v:
Clinical and Applied Thrombosis/Hemostasis, Vol 28 (2022)
Introduction Protease activated receptors 1 (PAR1) and 4 (PAR4) agonists are used to study platelet activation. Data on platelet activation are extrapolated across experimental settings. C1-inhibitor (C1INH) is a protease inhibitor present in plasma
Externí odkaz:
https://doaj.org/article/c02ef28fa6884a4193916003ebf2cc9d