Zobrazeno 1 - 10
of 63
pro vyhledávání: '"Paul V. C. Hough"'
Publikováno v:
Biophysical Journal. 66(2):293-298
For inorganic crystals such as calcite (CaCO3), Atomic Force Microscopy (AFM) has provided surface structure at atomic resolution (Ohnesorge and Binnig, 1993). As part of a broad effort to obtain high resolution for an individual protein or protein a
Autor:
Virgil B. Elings, Magdalena Bezanilla, Jay C. Groppe, Paul V. C. Hough, Helen G. Hansma, G. Gurley, Robert L. Sinsheimer, Paul K. Hansma, Iris A. Mastrangelo, Thomas C. Bruice
Publikováno v:
Scanning. 15:296-299
Three advances involving DNA in atomic force microscopy (AFM) are reported here. First a HEPES-Mg buffer has been used that improves the spreading of DNA and provides good DNA coverage with as little as 200-500 picograms per sample. Second, the new "
Publikováno v:
Proceedings of the National Academy of Sciences. 88:5670-5674
Using conventional and scanning transmission electron microscopy, we have examined the physical basis of long-range enhancer effects between distal and proximal elements in a eukaryotic promoter. Specifically, we have studied binding of human transcr
Autor:
Nicholas H. Heintz, Nathaniel Heintz, Lisa Dailey, Joseph S. Wall, Paul Held, Iris A. Mastrangelo, Paul V. C. Hough
Publikováno v:
Journal of molecular biology. 232(3)
We show assembly of low and high multimers of HeLa cell nuclear protein, RIP60, at the origin of bidirectional replication (OBR) identified by Burhans, Vassilev, Caddle, Heintz and DePamphilis in Chinese hamster ovary cells. RIP60 binds a 5′-ATT-3
Autor:
Joseph S. Wall, James F. Hainfeld, P Tegtmeyer, Paul V. C. Hough, Van G. Wilson, Iris A. Mastrangelo
Publikováno v:
Proceedings of the National Academy of Sciences. 82:3626-3630
Large tumor (T) antigen and its bound multimeric states are positioned by scanning transmission electron microscopy (STEM) within a few base pairs at control sequences of the simian virus 40 DNA origin of replication region. Proximal and distal edge
Autor:
Robert G. Roeder, Michele Sawadogo, James F. Hainfeld, Iris A. Mastrangelo, Joseph S. Wall, Paul V. C. Hough
Publikováno v:
Proceedings of the National Academy of Sciences. 84:4826-4830
The gene-specific transcription initiation factor USF (upstream stimulatory factor) binds at a palindromic sequence that extends from -52 to -63 relative to the start site of the adenovirus type 2 major late promoter; USF enhances in vitro transcript
Autor:
Wayne R. McKinney, Paul V. C. Hough
Publikováno v:
Journal of Microscopy. 122:193-207
Biological structures not seen by conventional light microscopy, such as longitudinal striations in polytene chromosomes, and, at the limit of sensitivity, virions of adenovirus 2, have been detected via DNA-associated fluorescence excited under the
Autor:
Kevin Ryder, Joseph S. Wall, Van G. Wilson, Iris A. Mastrangelo, James F. Hainfeld, P Tegtmeyer, Paul V. C. Hough
Publikováno v:
Nature Biotechnology. 3:549-553
We review a current methodology which analyzes quantitative scattering data from Scanning Transmission Electron Microscopy to establish footprints—positions of DNA-binding proteins on cloned genes—and mass distributions for the bound protein. Rec
Autor:
Alvin H. Bachman, Kwan Wu Lai, I. O. Skillicorn, J. Michael Scarr, R. M. Lea, Thomas G. Schumann, M. S. Webster, David J. Crennell, Paul V. C. Hough, G.R. Kalbfleisch, R. C. Strand, Philip Baumel
Publikováno v:
Physical Review Letters. 18:323-327
Autor:
W. M. Bugg, I. R. Kenyon, H. O. Cohn, G. T. Condo, R.D. McCulloch, M. M. Nussbaum, I. O. Skillicorn, A. M. Cnops, J. M. Scarr, Paul V. C. Hough, F.R. Huson
Publikováno v:
Physical Review Letters. 21:1609-1612
A diffractively produced $3\ensuremath{\pi}$ enhancement is observed with mass \ensuremath{\sim}1.1 GeV and width \ensuremath{\sim}0.35 GeV. There is no structure observed in the peak in either the mass or angular distributions. The system is predomi