Zobrazeno 1 - 10
of 42
pro vyhledávání: '"Paul Skehel"'
Autor:
Stella Kouloulia, Erik I. Hallin, Konstanze Simbriger, Inês S. Amorim, Gilliard Lach, Theoklitos Amvrosiadis, Kleanthi Chalkiadaki, Agniete Kampaite, Vinh Tai Truong, Mehdi Hooshmandi, Seyed Mehdi Jafarnejad, Paul Skehel, Petri Kursula, Arkady Khoutorsky, Christos G. Gkogkas
Publikováno v:
Cell Reports, Vol 29, Iss 11, Pp 3620-3635.e7 (2019)
Summary: The translation initiation repressor 4E-BP2 is deamidated in the brain on asparagines N99/N102 during early postnatal brain development. This post-translational modification enhances 4E-BP2 association with Raptor, a central component of mTO
Externí odkaz:
https://doaj.org/article/e93e84be921b440888508dbe8ce36c17
Autor:
Yoshiki Miyasaka, Yoshihiro Uno, Kazuto Yoshimi, Yayoi Kunihiro, Takuji Yoshimura, Tomohiro Tanaka, Harumi Ishikubo, Yuichi Hiraoka, Norihiko Takemoto, Takao Tanaka, Yoshihiro Ooguchi, Paul Skehel, Tomomi Aida, Junji Takeda, Tomoji Mashimo
Publikováno v:
BMC Genomics, Vol 19, Iss 1, Pp 1-8 (2018)
Abstract Background CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Results Here, we show that the electroporation of Cas9, two gRNAs, and long single-strand
Externí odkaz:
https://doaj.org/article/226795467b9f47eebef2789b9696428e
Autor:
Laura C. Graham, Samantha L. Eaton, Paula J. Brunton, Abdelmadjid Atrih, Colin Smith, Douglas J. Lamont, Thomas H. Gillingwater, Giuseppa Pennetta, Paul Skehel, Thomas M. Wishart
Publikováno v:
Molecular Neurodegeneration, Vol 12, Iss 1, Pp 1-16 (2017)
Abstract Background Neurons are highly polarized cells consisting of three distinct functional domains: the cell body (and associated dendrites), the axon and the synapse. Previously, it was believed that the clinical phenotypes of neurodegenerative
Externí odkaz:
https://doaj.org/article/0e5d6236a27f4b718ca0a780e7bcc4c4
Publikováno v:
Wellcome Open Research, Vol 2 (2017)
Background: The N-methyl-D-aspartate receptor (NMDAR) is an ionotropic glutamate receptor that has important roles in synaptogenesis, synaptic transmission, and synaptic plasticity. Recently, a large number of rare genetic variants have been found in
Externí odkaz:
https://doaj.org/article/c562eb2a2b194432b69f48efb505867b
Publikováno v:
Wellcome Open Research, Vol 2 (2017)
Background: The N-methyl-D-aspartate receptor (NMDAR) is an ionotropic glutamate receptor that has important roles in synaptogenesis, synaptic transmission, and synaptic plasticity. Recently, a large number of rare genetic variants have been found in
Externí odkaz:
https://doaj.org/article/e5d9b0fe164f473a8e17e9b6cf1392f2
Autor:
Guy C. C. Allen, Leire M. Ledahawsky, Elizabeth R. Evans, Molly Scott, Kosala N. Dissanayake, Clifford C. Shone, Paul Skehel, Veronika Boczonadi, Richard R. Ribchester, Ross A. Jones, Cornelia Roesl, Thomas M. Wishart, Graeme R. Jordan, Clarke R. Slater, Richard J. Mead, David Hughes, Alanna Thomson, Thomas H. Gillingwater
Publikováno v:
Biomolecules, Vol 11, Iss 1499, p 1499 (2021)
Biomolecules
Volume 11
Issue 10
Biomolecules
Volume 11
Issue 10
Live imaging of neuromuscular junctions (NMJs) in situ has been constrained by the suitability of ligands for inert vital staining of motor nerve terminals. Here, we constructed several truncated derivatives of the tetanus toxin C-fragment (TetC) fus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc974dd20c37630d62d0733dbc06e335
https://www.mdpi.com/2218-273X/11/10/1499
https://www.mdpi.com/2218-273X/11/10/1499
Publikováno v:
Brain Communications. 3
Primary hippocampal cell cultures are routinely used as an experimentally accessible model platform for the hippocampus and brain tissue in general. Containing multiple cell types including neurons, astrocytes and microglia in a state that can be rea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5f19905b906a50af2bd664ca381fcff7
https://doi.org/10.1093/braincomms/fcab152
https://doi.org/10.1093/braincomms/fcab152
Autor:
Thomas H. Gillingwater, Paul Skehel, Thomas M. Wishart, Neil A. Mabbott, Laura C. Graham, Rachel A. Kline, Douglas J. Lamont
Publikováno v:
Cells
Graham, L C, Kline, R A, Lamont, D J, Gillingwater, T H, Mabbott, N A, Skehel, P A & Wishart, T M 2021, ' Temporal Profiling of the Cortical Synaptic Mitochondrial Proteome Identifies Ageing Associated Regulators of Stability ', Cells, vol. 10, no. 12 . https://doi.org/10.3390/cells10123403
Cells; Volume 10; Issue 12; Pages: 3403
Cells, Vol 10, Iss 3403, p 3403 (2021)
Graham, L C, Kline, R A, Lamont, D J, Gillingwater, T H, Mabbott, N A, Skehel, P A & Wishart, T M 2021, ' Temporal Profiling of the Cortical Synaptic Mitochondrial Proteome Identifies Ageing Associated Regulators of Stability ', Cells, vol. 10, no. 12 . https://doi.org/10.3390/cells10123403
Cells; Volume 10; Issue 12; Pages: 3403
Cells, Vol 10, Iss 3403, p 3403 (2021)
Synapses are particularly susceptible to the effects of advancing age, and mitochondria have long been implicated as organelles contributing to this compartmental vulnerability. Despite this, the mitochondrial molecular cascades promoting age-depende
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15aee667ea0c00cc8752fed1ef7145ba
https://doi.org/10.3390/cells10123403
https://doi.org/10.3390/cells10123403
Autor:
Petri Kursula, Mehdi Hooshmandi, Paul Skehel, Inês S. Amorim, Arkady Khoutorsky, Seyed Mehdi Jafarnejad, Konstanze Simbriger, Vinh Tai Truong, Agniete Kampaite, Stella Kouloulia, Gilliard Lach, Christos G. Gkogkas, Erik Ingmar-Hallin, Theoklitos Amvrosiadis
Publikováno v:
SSRN Electronic Journal.
The translation initiation repressor 4E-BP2 is deamidated in brain on asparagines N99/N102 during early postnatal brain development. This post-translational modification enhances 4E-BP2 association with Raptor, a central component of mTORC1 and alter
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0aa9f24841025ff92f67ed355a9fe8a9
https://doi.org/10.2139/ssrn.3396492
https://doi.org/10.2139/ssrn.3396492
Autor:
Norihiko Takemoto, Yuichi Hiraoka, Yoshihiro Uno, Yoshihiro Ooguchi, Tomoji Mashimo, Tomohiro Tanaka, Kazuto Yoshimi, Junji Takeda, Yoshiki Miyasaka, Harumi Ishikubo, Tomomi Aida, Takuji Yoshimura, Takao Tanaka, Yayoi Kunihiro, Paul Skehel
Publikováno v:
BMC Genomics, Vol 19, Iss 1, Pp 1-8 (2018)
BMC Genomics
Miyasaka, Y, Uno, Y, Yoshimi, K, Kunihiro, Y, Yoshimura, T, Tanaka, T, Ishikubo, H, Hiraoka, Y, Takemoto, N, Tanaka, T, Ooguchi, Y, Skehel, P, Aida, T, Takeda, J & Mashimo, T 2018, ' CLICK : One-step generation of conditional knockout mice ', BMC Genomics, vol. 19, no. 1, 318 . https://doi.org/10.1186/s12864-018-4713-y
BMC Genomics
Miyasaka, Y, Uno, Y, Yoshimi, K, Kunihiro, Y, Yoshimura, T, Tanaka, T, Ishikubo, H, Hiraoka, Y, Takemoto, N, Tanaka, T, Ooguchi, Y, Skehel, P, Aida, T, Takeda, J & Mashimo, T 2018, ' CLICK : One-step generation of conditional knockout mice ', BMC Genomics, vol. 19, no. 1, 318 . https://doi.org/10.1186/s12864-018-4713-y
Background CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Results Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc9d00a2ce1d27f99b4f0889793e0fa0
https://doi.org/10.1186/s12864-018-4713-y
https://doi.org/10.1186/s12864-018-4713-y