Zobrazeno 1 - 10
of 60
pro vyhledávání: '"Paul S. Sypherd"'
Publikováno v:
Infection and Immunity. 61:3648-3655
The gene ECE1 (extent of cell elongation 1) was isolated by differential hybridization screening of a Candida albicans cDNA library by using probes derived from populations of yeast cells or hyphae. Expression of this gene was not detected when C. al
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c57cfb9e368356306ece247da8668c50
https://doi.org/10.1128/iai.61.9.3648-3655.1993
https://doi.org/10.1128/iai.61.9.3648-3655.1993
Autor:
Susan M. Saporito, Paul S. Sypherd
Publikováno v:
Gene. 106:43-49
Candida albicans is a dimorphic, opportunistic pathogen of humans, and calcium and calmodulin have been implicated in its morphogenic transition. The C. albicans calmodulin-encoding gene, CMD1, was isolated from cDNA and genomic phage λ libraries us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4be32a171e71e0c355c4d191f605cbd7
https://doi.org/10.1016/0378-1119(91)90564-r
https://doi.org/10.1016/0378-1119(91)90564-r
Publikováno v:
Journal of Bacteriology. 172:2036-2045
Two Candida albicans genes that encode the protein synthesis factor elongation factor 1 alpha (EF-1 alpha) were cloned by using a heterologous TEF1 probe from Mucor racemosus to screen libraries of C. albicans genomic DNA. Sequence analysis of the tw
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f63935c801c4d35fe1cd523935666f58
https://doi.org/10.1128/jb.172.4.2036-2045.1990
https://doi.org/10.1128/jb.172.4.2036-2045.1990
Publikováno v:
Current genetics. 27(3)
The C. albicans URA3 gene was tested as a reporter of gene expression. An integrating vector was constructed which contained ADE2 as a selectable marker together with a truncated form of URA3 lacking the first three codons. A DNA fragment containing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::baa862a7de68af5d2b66b105b0ac128e
https://pubmed.ncbi.nlm.nih.gov/7736609
https://pubmed.ncbi.nlm.nih.gov/7736609
Publikováno v:
Nucleic acids research. 20(7)
Elongation factor 3 (EF-3) is a unique and essential component of the translational system in fungi. The gene, CEF-3, encoding elongation factor 3 has been isolated from the dimorphic fungus Candida albicans. A heterologous gene probe containing the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e931a9e503d7dd198a96e6cb81ce3caa
https://pubmed.ncbi.nlm.nih.gov/1579463
https://pubmed.ncbi.nlm.nih.gov/1579463
Publikováno v:
Molecular microbiology. 5(7)
Summary In previous work, we showed that Candida albicans has two genes, TEF-1 and TEF-2, which encode identical polypeptides for the highly conserved, essential, protein synthesis factor EF-1α (Breviario et al., 1988). This result prompted question
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1db995fcaaa1cba2711e691421124f7
https://pubmed.ncbi.nlm.nih.gov/1943704
https://pubmed.ncbi.nlm.nih.gov/1943704
Autor:
John W. Wireman, Paul S. Sypherd
Publikováno v:
Biochemistry. 13:1215-1221
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18457e8d8cc9575fff5dbcd730114b4f
https://doi.org/10.1021/bi00703a025
https://doi.org/10.1021/bi00703a025
Publikováno v:
Biochemical and Biophysical Research Communications. 61:322-328
Electrophoresis of ribosomal proteins from 1-day regenerating and sham-operated rat livers, doubly-labeled with two iodine isotopes, revealed that the relative stoichiometric ratios of proteins S6 from the 40S subunit and L5, L18 and L27 from the 60S
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d5d0de90d3608902587123aeeb14400
https://doi.org/10.1016/0006-291x(74)90569-5
https://doi.org/10.1016/0006-291x(74)90569-5
Publikováno v:
Nucleic Acids Research. 15:9997-10006
Our previous studies have shown that Mucor racemosus possesses three genes (TEF-1, -2 and -3) for EF-1 alpha, and that all three genes are transcribed. However, the level of transcription varies markedly between the three genes, with TEF-1 mRNA level
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b45235e66f470dce041555ebc41798ce
https://doi.org/10.1093/nar/15.23.9997
https://doi.org/10.1093/nar/15.23.9997
Autor:
Paul S. Sypherd, Alf D. Larsen
Publikováno v:
Molecular and General Genetics MGG. 175:99-109
Ribosomal proteins of the dimorphic fungus Mucor racemosus were isolated and characterized by 2-dimensional gel electrophoresis. Proteins from ribosomes of the yeast and mycelial phase were compared, and were found to be qualitatively indistinguishab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae24d1d40809654477696e082c085e1d
https://doi.org/10.1007/bf00267861
https://doi.org/10.1007/bf00267861