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Autor:
Lindsay A. Horst, Michael J. Thomenius, Paul L. Edmiston, Betsy A. Kersteen, Michael J. Jourden, Guy B. Mulligan, Paul R. Geiss, Charles L. Borders, Nichole R. Myers, Ryan M. Almeida, Melissa M. Barty, Melissa J. Brym, Mark J. Snider
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1751:178-183
Six fully conserved arginine residues (R129, R131, R235, R291, R319, and R340) closely grouped in the nucleotide binding site of rabbit muscle creatine kinase (rmCK) were mutated; four to alanine and all six to lysine. Kinetic analyses in the directi