Protocol to use de-epithelialized porcine urinary bladder as a tissue scaffold for propagation of pancreatic cells

Autor: Michael Karl Melzer, Markus Breunig, Paul Lopatta, Meike Hohwieler, Sarah Merz, Anca Azoitei, Cagatay Günes, Christian Bolenz, Alexander Kleger

Publikováno v: STAR Protocols, Vol 3, Iss 4, Pp 101869- (2022)

Summary: Ex vivo organ culture can be a useful alternative to in vivo models, which can be time-, labor-, and cost-intensive. Here we describe a step-by-step protocol to use de-epithelialized porcine urinary bladders as scaffolds in air-liquid interf

Externí odkaz: https://doaj.org/article/5d8b4b8b8dd04c908cf9b27759361b14

Secreted retrovirus-like GAG-domain-containing protein PEG10 is regulated by UBE3A and is involved in Angelman syndrome pathophysiology

Autor: Paul Lopatta, Ravi Jagasia, Rasmussen Søren Vestergaard, Tom Dunkley, Marco Berrera, Nikhil J. Pandya, Edwin Mientjes, Tania Distler, Ben Distel, Balazs Banfai, F. Isabella Zampeta, Ype Elgersma, Philip Grossen, Christoph Patsch, Martin Ebeling, Thomas Kremer, Sonja Meier, Yasmina Martí, A. Mattijs Punt, Congwei Wang, Veronica Costa, Manuel Tzouros, Marius C. Hoener

Publikováno v: Cell Reports Medicine
Cell Reports Medicine, 2(8):100360. Cell Press

Angelman syndrome (AS) is a neurodevelopmental disorder caused by the loss of maternal UBE3A, a ubiquitin protein ligase E3A. Here, we study neurons derived from patients with AS and neurotypical individuals, and reciprocally modulate UBE3A using ant

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1738738cb51a3998314b1853efed0fa
https://doi.org/10.1016/j.xcrm.2021.100360

ADAM9 contributes to vascular invasion in pancreatic ductal adenocarcinoma

Autor: Birte Kulemann, Alexander Nyström, Jörg W. Bartsch, Jens Hoeppner, Paul Lopatta, Catharina Conrad, Victor O. Oria, Peter Bronsert, Oliver Schilling, Bogdan-Tiberius Preca, Tatjana Schmitz, Jochen Maurer

Publikováno v: Molecular Oncology
Molecular oncology 13(2), 456-479 (2019). doi:10.1002/1878-0261.12426
Molecular Oncology, Vol 13, Iss 2, Pp 456-479 (2019)

A disintegrin and a metalloprotease (ADAM)‐9 is a metzincin cell‐surface protease with strongly elevated expression in solid tumors, including pancreatic ductal adenocarcinoma (PDAC). In this study, we performed immunohistochemistry (IHC) of a ti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ffefbd7764d8dc221e057b3bfed0215
https://pubmed.ncbi.nlm.nih.gov/30556643