Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Paul J. Steiner"'
Autor:
Brian M. Petersen, Monica B. Kirby, Karson M. Chrispens, Olivia M. Irvin, Isabell K. Strawn, Cyrus M. Haas, Alexis M. Walker, Zachary T. Baumer, Sophia A. Ulmer, Edgardo Ayala, Emily R. Rhodes, Jenna J. Guthmiller, Paul J. Steiner, Timothy A. Whitehead
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract Antibodies are engineerable quantities in medicine. Learning antibody molecular recognition would enable the in silico design of high affinity binders against nearly any proteinaceous surface. Yet, publicly available experiment antibody sequ
Externí odkaz:
https://doaj.org/article/ad885af6bfcd4b0ba2768a7ecb7b47e4
Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning
Publikováno v:
STAR Protocols, Vol 2, Iss 4, Pp 100869- (2021)
Summary: Here, we describe a protocol to identify escape mutants on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) receptor-binding domain (RBD) using a yeast screen combined with deep mutational scanning. Over 90% of all
Externí odkaz:
https://doaj.org/article/d3456cf5f4d347308c52ef2bfb2c0080
Autor:
Bailey B. Banach, Gabriele Cerutti, Ahmed S. Fahad, Chen-Hsiang Shen, Matheus Oliveira De Souza, Phinikoula S. Katsamba, Yaroslav Tsybovsky, Pengfei Wang, Manoj S. Nair, Yaoxing Huang, Irene M. Francino-Urdániz, Paul J. Steiner, Matías Gutiérrez-González, Lihong Liu, Sheila N. López Acevedo, Alexandra F. Nazzari, Jacy R. Wolfe, Yang Luo, Adam S. Olia, I-Ting Teng, Jian Yu, Tongqing Zhou, Eswar R. Reddem, Jude Bimela, Xiaoli Pan, Bharat Madan, Amy D. Laflin, Rajani Nimrania, Kwok-Yung Yuen, Timothy A. Whitehead, David D. Ho, Peter D. Kwong, Lawrence Shapiro, Brandon J. DeKosky
Publikováno v:
Cell Reports, Vol 37, Iss 1, Pp 109771- (2021)
Summary: Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as a member of a p
Externí odkaz:
https://doaj.org/article/eeb91ec1080042a7abf186e7a830013e
Construction of user-defined long circular single stranded DNA (cssDNA) and linear single stranded DNA (lssDNA) is important for various biotechnological applications. Many current methods for synthesis of these ssDNA molecules do not scale to multi-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5ac30b7472a92f7e17f14934199ee51c
https://doi.org/10.1101/2022.11.21.517425
https://doi.org/10.1101/2022.11.21.517425
Autor:
Paul J. Steiner, Samuel D. Swift, Matthew Bedewitz, Ian Wheeldon, Sean R. Cutler, Dmitri A. Nusinow, Timothy A. Whitehead
Publikováno v:
Biochemistry.
Chemical-induced dimerization (CID) modules enable users to implement ligand-controlled cellular and biochemical functions for a number of problems in basic and applied biology. A special class of CID modules occur naturally in plants and involve a h
Autor:
Jesús Beltrán, Paul J. Steiner, Matthew Bedewitz, Shuang Wei, Francis C. Peterson, Zongbo Li, Brigid E. Hughes, Zachary Hartley, Nicholas R. Robertson, Angélica V. Medina-Cucurella, Zachary T. Baumer, Alison C. Leonard, Sang-Youl Park, Brian F. Volkman, Dmitri A. Nusinow, Wenwan Zhong, Ian Wheeldon, Sean R. Cutler, Timothy A. Whitehead
Publikováno v:
Nature biotechnology, vol 40, iss 12
A general method to generate biosensors for user-defined molecules could provide detection tools for a wide range of biological applications. Here, we describe an approach for the rapid engineering of biosensors using PYR1 (Pyrabactin Resistance 1),
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab8d23f4708788f4e65d99e7d13434f5
Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning
Publikováno v:
STAR Protocols, Vol 2, Iss 4, Pp 100869-(2021)
STAR Protocols
STAR Protocols
Here we describe a protocol to identify escape mutants on the SARS-CoV-2 Spike (S) receptor binding domain (RBD) using a yeast screen combined with deep mutational scanning. Over 90% of all potential single S RBD escape mutants can be identified for
Autor:
Linghang Peng, Paul J. Steiner, Irene M. Francino-Urdaniz, Alison C. Leonard, Shawn Barman, Emily R. Rhodes, Cyrus M. Haas, Fangzhu Zhao, Monica B Kirby, Joseph G. Jardine, Timothy A. Whitehead, Kayla G. Sprenger
Publikováno v:
bioRxiv
article-version (status) pre
article-version (number) 1
Cell Reports
article-version (status) pre
article-version (number) 1
Cell Reports
The potential emergence of SARS-CoV-2 Spike (S) escape mutants is a threat to reduce the efficacy of existing vaccines and neutralizing antibody (nAb) therapies. An understanding of the antibody/S escape mutation landscape is urgently needed to preem
Autor:
Sean R. Cutler, Matthew S. Faber, Paul J. Steiner, Alexandra N. Borelli, Jesús Beltrán, Angélica V Medina-Cucurella, Monica B Kirby, Timothy A. Whitehead
Publikováno v:
Protein Eng Des Sel
User-defined mutagenic libraries are fundamental for applied protein engineering workflows. Here we show that unamplified oligo pools can be used to prepare site saturation mutagenesis libraries from plasmid DNA with near-complete coverage of desired
Publikováno v:
Bio Protoc
Saturation mutagenesis is a fundamental enabling technology for protein engineering and epitope mapping. Nicking mutagenesis (NM) allows the user to rapidly construct libraries of all possible single mutations in a target protein sequence from plasmi