Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Paul J. Mcdonald"'
Publikováno v:
Engineering in Life Sciences. 16:93-98
Companies have adapted high-throughput techniques for process development to different levels. Some companies are beginning to incorporate high-throughput process development (HTPD) techniques at a level which is approachable for the beginner user (e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8e9603ce434fef6035266949ee93aed7
https://doi.org/10.1002/elsc.201400254
https://doi.org/10.1002/elsc.201400254
Autor:
Christopher Williams, Ti Zhao, Benjamin Tran, Brian D. Kelley, Paul J. Mcdonald, Marc Wong, Philip Lester
Publikováno v:
Journal of Chromatography A. 1433:66-74
Cation-exchange chromatography is widely used in the purification of therapeutic antibodies, wherein parameters such as elution pH and counterion concentration require optimization for individual antibodies across different chromatography resins. Wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::873b215c3172319c84e9493cced24ed2
https://doi.org/10.1016/j.chroma.2015.12.071
https://doi.org/10.1016/j.chroma.2015.12.071
Autor:
Christoph Spiess, Hok Seon Kim, William J. Galush, Isidro Hötzel, Nisana Andersen, Paul J. Mcdonald, Avinash Gill, Samarkand A. Estee, Greg A. Lazar, Sharon Viajar, Ankai Xu
Publikováno v:
Molecular pharmaceutics. 15(10)
A critical part of the clinical development path for a therapeutic antibody involves evaluating the physical and chemical stability of candidate molecules throughout the manufacturing process. In particular, the risks of chemical liabilities that can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37103092ff2b5eb2d5c7387e7850af20
https://pubmed.ncbi.nlm.nih.gov/30118239
https://pubmed.ncbi.nlm.nih.gov/30118239
Publikováno v:
Biotechnology Progress. 26:1322-1331
The potential of cationic polyelectrolytes to precipitate host cell and process related impurities was investigated, to replace one or more chromatography steps in monoclonal antibody purification. The impact of antibody isoelectric point, solution p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c55e39a0ed871cec4f1e505b2219202
https://doi.org/10.1002/btpr.437
https://doi.org/10.1002/btpr.437
Autor:
Donald E. Walker, Paul J. Mcdonald, Christopher Yu, Vanessa Grosskopf, Xiangdan Wang, Jihong Yang, Benjamin Tran
Publikováno v:
Journal of chromatography. A. 1438
Purification processes for therapeutic antibodies typically exploit multiple and orthogonal chromatography steps in order to remove impurities, such as host-cell proteins. While the majority of host-cell proteins are cleared through purification proc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b072a9b573905905868143b82d05c594
https://pubmed.ncbi.nlm.nih.gov/26896920
https://pubmed.ncbi.nlm.nih.gov/26896920
Publikováno v:
Biotechnology journal. 7(10)
Protein A chromatography is typically used as the initial capture step in the purification of monoclonal antibodies produced in Chinese hamster ovary (CHO) cells. Although exploiting an affinity interaction for purification, the level of host cell pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5dbf92a84c24c80a52e82aacdaca412
https://pubmed.ncbi.nlm.nih.gov/22623327
https://pubmed.ncbi.nlm.nih.gov/22623327
Autor:
Helga Raab, Richard H. Scheller, Reina N. Fuji, Suzie J. Scales, Ola Saad, Darshana Ramesh Patel, Leslie A. Khawli, Willy Solis, Allen J. Ebens, Charles Eigenbrot, Dongwei Li, Janet Tien, Mark X. Sliwkowski, Elaine Mai, Luna Liu, Paul Polakis, Paul J. Mcdonald, Susan D. Spencer, Surinder Kaur, Shang-Fan Yu, Margaret Kenrick, Wai Lee Wong, Jay Tibbitts, Trung Nguyen, Philip E. Hass, Sunil Bhakta, Jakub Baudys, Richard Vandlen, Kelly Flagella, Jagath Reddy Junutula, Kathryn L Parsons-Reponte, Keyang Xu, Ben-Quan Shen
Publikováno v:
Nature biotechnology. 30(2)
The reactive thiol in cysteine is used for coupling maleimide linkers in the generation of antibody conjugates. To assess the impact of the conjugation site, we engineered cysteines into a therapeutic HER2/neu antibody at three sites differing in sol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e18b8d32aebd8b50549f9d8edaf3f1bd
https://pubmed.ncbi.nlm.nih.gov/22267010
https://pubmed.ncbi.nlm.nih.gov/22267010
Publikováno v:
Biotechnology and bioengineering. 102(4)
We evaluated the potential for polyelectrolyte induced precipitation of antibodies to replace traditional chromatography purification. We investigated the impact of solution pH, solution ionic strength and polyelectrolyte molecular weight on the degr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed53546d526156a8b1289c90874171f6
https://pubmed.ncbi.nlm.nih.gov/19031425
https://pubmed.ncbi.nlm.nih.gov/19031425
Publikováno v:
Journal of chromatography. A. 1163(1-2)
Protein A affinity chromatography is a common method for process scale purification of monoclonal antibodies. During protein A affinity chromatography, protein A ligand co-elutes with the antibody (commonly called leaching), which is a potential disa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f85cda90cebb488c876b5a1d4e34233e
https://pubmed.ncbi.nlm.nih.gov/17643441
https://pubmed.ncbi.nlm.nih.gov/17643441
Autor:
Lisa A. Damico, Wai Lee T. Wong, Allen Nguyen, Arthur E. Reyes, Mark S. Dennis, Min Zhang, Paul J. Mcdonald
Publikováno v:
Protein engineering, designselection : PEDS. 19(7)
An AB.Fab (albumin-binding Fab) consists of a Fab and a phage-derived albumin-binding peptide. This molecule is capable of binding both antigen and albumin simultaneously. Using a Fab derived from Herceptin we generated a panel of AB.Fab variants wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1d5c9079b4c216ab27da719025c063c
https://pubmed.ncbi.nlm.nih.gov/16621915
https://pubmed.ncbi.nlm.nih.gov/16621915