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pro vyhledávání: '"Paul J. Goodwin"'
Autor:
William N. Lanzilotta, Dennis R. Dean, Paul W. Ludden, Paul J. Goodwin, Priya Rangaraj, Matthew J. Ryle, Vinod K. Shah
Publikováno v:
Journal of Biological Chemistry. 274:29413-29419
Besides serving as the obligate electron donor to dinitrogenase during nitrogenase turnover, dinitrogenase reductase (NifH) is required for the biosynthesis of the iron-molybdenum cofactor (FeMo-co) and for the maturation of alpha(2)beta(2) apo-dinit
Autor:
Paul J. Goodwin, Lance C. Seefeldt, Jason Christiansen, William N. Lanzilotta, Dennis R. Dean
Publikováno v:
Biochemistry. 37:12611-12623
A Zn-immobilized metal-affinity chromatography technique was used to purify a poly-histidine-tagged, FeMo-cofactorless MoFe protein (apo-MoFe protein) from a nifB-deletion mutant of Azotobacter vinelandii. Apo-MoFe protein prepared in this way was ob
Autor:
Jeffrey N. Agar, Dennis R. Dean, Michael K. Johnson, Paul J. Goodwin, Jon T. Roll, Gary P. Roberts
Publikováno v:
Biochemistry. 37:10420-10428
The nifE and nifN gene products from Azotobacter vinelandii form an alpha2beta2 tetramer (NifEN complex) that is required for the biosynthesis of the nitrogenase FeMo cofactor. In the current model for NifEN complex organization and function, the com