Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

Autor: Arthur Abelian, Thomas Mund, Martin D. Curran, Stuart A. Savill, Nipa Mitra, Carol Charan, Amanda L. Ogilvy-Stuart, Hugh R. B. Pelham, Paul H. Dear

Publikováno v: BMC Infectious Diseases, Vol 20, Iss 1, Pp 1-11 (2020)

Abstract Background PCRctic is an innovative assay based on 16S rDNA PCR technology that has been designed to detect a single intact bacterium in a specimen of cerebro-spinal fluid (CSF). The assay’s potential for accurate, fast and inexpensive dis

Externí odkaz: https://doaj.org/article/69d6ef3396dd4b6ba0e8579d0f06fd5d

Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Autor: Eileen P Hamilton, Aurélie Kapusta, Piroska E Huvos, Shelby L Bidwell, Nikhat Zafar, Haibao Tang, Michalis Hadjithomas, Vivek Krishnakumar, Jonathan H Badger, Elisabet V Caler, Carsten Russ, Qiandong Zeng, Lin Fan, Joshua Z Levin, Terrance Shea, Sarah K Young, Ryan Hegarty, Riza Daza, Sharvari Gujja, Jennifer R Wortman, Bruce W Birren, Chad Nusbaum, Jainy Thomas, Clayton M Carey, Ellen J Pritham, Cédric Feschotte, Tomoko Noto, Kazufumi Mochizuki, Romeo Papazyan, Sean D Taverna, Paul H Dear, Donna M Cassidy-Hanley, Jie Xiong, Wei Miao, Eduardo Orias, Robert S Coyne

Publikováno v: eLife, Vol 5 (2016)

The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analy

Externí odkaz: https://doaj.org/article/5b8a3b25eb90453aad49d3598e13d79e

Single-molecule detection of deoxyribonucleoside triphosphates in microdroplets

Autor: David M. Love, James Bush, Fabian Tolle, Lindsey A Ibbotson, Boris Breiner, Neil M. Bell, Aya Shibahara, Ana-Luisa Silva, Olympia Pachoumi, Kerr Johnson, Cameron Alexander Frayling, Magdalena Stolarek, Michele Amasio, Leigh R Shelford, Podd Gareth, Mei Wu, Maciej Sosna, Barnaby Balmforth, Aurel Negrea, Douglas J Kelly, Sheridan Eoin, Edoardo Petrini, Tom H Isaac, Jasmin Chana, Paul H. Dear, Monica S Saavedra, Palmer Rebecca, Alexander Dunning, Mark Dethlefsen, Jekaterina Kuleshova

Publikováno v: Nucleic Acids Research

A new approach to single-molecule DNA sequencing in which dNTPs, released by pyrophosphorolysis from the strand to be sequenced, are captured in microdroplets and read directly could have substantial advantages over current sequence-by-synthesis meth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03b4f82429790f363dfb4d9414364712
https://pubmed.ncbi.nlm.nih.gov/31318971

IRS2 is a candidate driver oncogene on 13q34 in colorectal cancer

Autor: Frank McCaughan, Shani A. Mulholland, George Poulogiannis, Elizabeth Day, Mark J. Arends, Ashraf E. K. Ibrahim, Paul H. Dear

Publikováno v: International Journal of Experimental Pathology
Day, E, Poulogiannis, G, McCaughan, F, Mulholland, S, Arends, M J, Ibrahim, A E K & Dear, P H 2013, ' IRS2 is a candidate driver oncogene on 13q34 in colorectal cancer ', International Journal of Experimental Pathology, vol. 94, no. 3, pp. 203-11 . https://doi.org/10.1111/iep.12021

Copy number alterations are frequently found in colorectal cancer (CRC), and recurrent gains or losses are likely to correspond to regions harbouring genes that promote or impede carcinogenesis respectively. Gain of chromosome 13q is common in CRC bu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cee0a0a068aacb87f15cf9bebfb13f69
http://europepmc.org/articles/PMC3664965

Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Autor: Jie Xiong, Riza M. Daza, Robert S. Coyne, Nikhat Zafar, Tomoko Noto, Lin Fan, Eduardo Orias, Eileen P. Hamilton, Cédric Feschotte, Donna Cassidy-Hanley, Jennifer R. Wortman, Michalis Hadjithomas, Piroska Huvos, Wei Miao, Joshua Z. Levin, Jonathan H. Badger, Romeo Papazyan, Jainy Thomas, Vivek Krishnakumar, Clayton M. Carey, Sharvari Gujja, Sarah Young, Qiandong Zeng, Ryan Hegarty, Paul H. Dear, Terrance Shea, Elisabet Caler, Aurélie Kapusta, Chad Nusbaum, Ellen J. Pritham, Haibao Tang, Shelby L. Bidwell, Kazufumi Mochizuki, Bruce W. Birren, Sean D. Taverna, Carsten Russ

Publikováno v: eLife, Vol 5 (2016)

The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91982593fcac04b4d64d5f7345221c07
https://elifesciences.org/articles/19090