Preparation, FPLC Purification and LC-FT-ICR-MS of Proteins

Autor: Paul H. Blum, Jiri Adamec, Tyler B. Johnson

Publikováno v: Bio Protoc

High magnetic field Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers provide extremely high mass resolution (resolving power of ~200,000 at 400 m/z) protein detection across a broad mass range, enabling analysis of fine structure

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::148d143f072f2881ffe67d48ac7b9387
https://doi.org/10.21769/bioprotoc.3570

Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution

Autor: Anna Lipzen, Paul H. Blum, Erica Keffeler, Joel Martin, Wendy Schackwitz, Samuel McCarthy, Tyler B. Johnson, Sophie Payne, Benjamin J. Pavlik

Publikováno v: McCarthy, S; Johnson, T; Pavlik, BJ; Payne, S; Schackwitz, W; Martin, J; et al.(2016). Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(3), 857-867. doi: 10.1128/AEM.03225-15. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/20r874k6
Applied and environmental microbiology, vol 82, iss 3

Extremely thermoacidophilic Crenarchaeota belonging to the order Sulfolobales flourish in hot acidic habitats that are strongly oxidizing. The pH extremes of these habitats, however, often exceed the acid tolerance of type species and strains. Here,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8413b90dd4095461abbd5318bd6262e
https://doi.org/10.1128/aem.03225-15

Uncoupling Fermentative Synthesis of Molecular Hydrogen from Biomass Formation in Thermotoga maritima

Autor: Derrick White, Raghuveer Singh, Paul H. Blum, Robert M. Kelly, Kenneth M. Noll, Yaşar Demirel

When carbohydrates are fermented by the hyperthermophilic anaerobe Thermotoga maritima, molecular hydrogen (H2) is formed in strict proportion to substrate availability. Excretion of the organic acids acetate and lactate provide an additional sink fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89b05f2271f6dd056a924dd62e207f8b
https://europepmc.org/articles/PMC6102995/

Membrane Association and Catabolite Repression of the Sulfolobus solfataricus α-Amylase

Autor: Paul H. Blum, Deepak Rudrappa, Edith Soo

Publikováno v: Microorganisms
Volume 3
Issue 3
Pages 567-587
Microorganisms, Vol 3, Iss 3, Pp 567-587 (2015)

Sulfolobus solfataricus is a thermoacidophilic member of the archaea whose envelope consists of an ether-linked lipid monolayer surrounded by a protein S-layer. Protein translocation across this envelope must accommodate a steep proton gradient that

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7691dcdbc8fe179179f18f476c5979be
https://doi.org/10.3390/microorganisms3030567

Effects of thermodynamically coupled reaction diffusion in microalgae growth and lipid accumulation: Model development and stability analysis

Autor: Deepak Rudrappa, Rahul Tevatia, Paul H. Blum, Yaşar Demirel

Publikováno v: Computers & Chemical Engineering. 75:28-39

This study investigates and presents the effects of thermodynamically coupled nonisothermal reaction-diffusion processes on microalgae growth, substrate consumption and neutral lipid production in a pond or wastewater treatment plant. The non-stirred

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7b979c260a5ab375103f05660d00cde4
https://doi.org/10.1016/j.compchemeng.2015.01.011