Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Paul H. Bessette"'
Autor:
Eleonora Trotta, Paul H. Bessette, Stephanie L. Silveria, Lauren K. Ely, Kevin Jude, Charles R. Holst, Anthony Coyle, Christopher K. Garcia, Natasha K. Crellin, Isaac J. Rondon, Jeffrey A. Bluestone
Publikováno v:
The Journal of Immunology. 200:176.4-176.4
Interleukin-2 (IL-2) has been shown to suppress immune pathologies by preferentially expanding regulatory T cells (Treg). However, this therapy has been limited by off-target complications due to pathogenic cell expansion. Recent efforts have been fo
Autor:
Jiangrong Qian, Paul H. Bessette, Hyongsok Tom Soh, Carl D. Meinhart, Patrick S. Daugherty, Xiaoyuan Hu
Publikováno v:
Proceedings of the National Academy of Sciences. 102:15757-15761
Current techniques in high-speed cell sorting are limited by the inherent coupling among three competing parameters of performance: throughput, purity, and rare cell recovery. Microfluidics provides an alternate strategy to decouple these parameters
Publikováno v:
Journal of Bacteriology. 183:980-988
We have examined the role of the active-site CXXC central dipeptides of DsbA and DsbC in disulfide bond formation and isomerization in the Escherichia coli periplasm. DsbA active-site mutants with a wide range of redox potentials were expressed eithe
Autor:
Fei Han, Shouchun Liu, Stephen James Moore, Jennifer Richardson, Jason Gee, Annie Yang, Olga Vasiljeva, Margaret Nguyen, Luc R. Desnoyers, Kenneth R. Wong, Kate Markham, Elizabeth Menendez, Kathy Kamath, Tony W. Liang, Jeanne Grace Flandez, Daniel R. Hostetter, James W. West, Chihunt Wong, Paul H. Bessette, Henry B. Lowman, Michael Krimm, Patrick S. Daugherty, Jason Gary Sagert
Publikováno v:
Science Translational Medicine. 5
Target-mediated toxicity constitutes a major limitation for the development of therapeutic antibodies. To redirect the activity of antibodies recognizing widely distributed targets to the site of disease, we have applied a prodrug strategy to create
Publikováno v:
Proceedings of the National Academy of Sciences. 96:13703-13708
Under physiological conditions, the Escherichia coli cytoplasm is maintained in a reduced state that strongly disfavors the formation of stable disulfide bonds in proteins. However, mutants in which the reduction of both thioredoxins and glutathione
Publikováno v:
Journal of Biological Chemistry. 274:7784-7792
We have characterized in vivo and in vitro the recently identified DsbG from Escherichia coli. In addition to sharing sequence homology with the thiol disulfide exchange protein DsbC, DsbG likewise was shown to form a stable periplasmic dimer, and it
Publikováno v:
Europe PubMed Central
The Escherichia coli periplasmic protein DsbC is active both in vivo and in vitro as a protein disulfide isomerase. For DsbC to attack incorrectly formed disulfide bonds in substrate proteins, its two active-site cysteines should be in the reduced fo
Publikováno v:
Analytical chemistry. 79(5)
The capability to screen molecular libraries using disposable microfluidic devices provides the potential to simplify and automate reagent generation and to develop integrated bioanalytical systems for clinical diagnostics. Here, antibody epitopes we
A bacterial display methodology was developed for N- and C-terminal display and demonstrated to enable rapid screening of very large peptide libraries with high precision and efficiency. To overcome limitations of insertional fusion display libraries
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1920c84072e36af5061861e1c7c11ba5
https://europepmc.org/articles/PMC2242469/
https://europepmc.org/articles/PMC2242469/
Autor:
David Kisailus, Meredith M. Murr, Paul Curnow, Paul H. Bessette, Patrick S. Daugherty, Daniel E. Morse
Publikováno v:
Journal of the American Chemical Society. 127(45)
We introduce a novel method of inorganic synthesis using the catalytic and structure-directing properties of the demosponge enzyme silicatein-alpha. Recombinant silicatein-alpha was displayed at the surface of Escherichia coli cells by fusion to oute