Výsledky vyhledávání - "Paul Framson"

New genetic techniques for group B streptococci: high-efficiency transformation, maintenance of temperature-sensitive pWV01 plasmids, and mutagenesis with Tn917

Autor: Craig E. Rubens, Julia Merry, Aphakorn Nittayajarn, Paul Framson, Philip Youngman

Publikováno v: Applied and Environmental Microbiology. 63:3539-3547

Three techniques were developed to improve the genetic manipulation of group B streptococci (GBS). We first optimized a protocol for transformation of GBS by electroporation, which provided transformation efficiencies of 10(5) CFU/microgram. Variable

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd246d128b65307aa0e5517fb56f7839
https://doi.org/10.1128/aem.63.9.3539-3547.1997

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Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression

Autor: Sreelekha Devarayalu, D Alfi, Paul Luo Li, Paul Framson, Paul Bornstein

Publikováno v: Journal of Biological Chemistry. 265:16691-16698

We have isolated the mouse thrombospondin (TS) gene and determined the DNA sequence of the first nine exons and eight introns. Comparison with the human cDNA sequence reveals a high degree of conservation in coding sequences. Exon 3 of the mouse gene

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be8689f9dd85d855491bb03546447e77
https://doi.org/10.1016/s0021-9258(17)46276-4

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A serum response element and a binding site for NF-Y mediate the serum response of the human thrombospondin 1 gene

Autor: Paul Framson, Paul Bornstein

Publikováno v: The Journal of biological chemistry. 268(7)

The expression of thrombospondin 1 (TSP 1), a member of the TSP gene family, is rapidly induced by growth factors. We tested the ability of human TSP 1-chloramphenicol acetyltransferase constructs to respond to serum in stably transfected NIH-3T3 cel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04068ab2a8d33eef22024d6920de4b3b
https://pubmed.ncbi.nlm.nih.gov/8444876

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A second thrombospondin gene in the mouse is similar in organization to thrombospondin 1 but does not respond to serum

Autor: Paul Luo Li, Sreelekha Devarayalu, Paul Framson, Paul Bornstein, Christine M. Disteche

A second, expressed thrombospondin (TSP) gene, Thbs2, has been identified in the mouse. The exon/intron organization of Thbs2 is highly conserved in comparison with Thbs1 in that exon size and the pattern of interruption of the reading frame by intro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f9f8c8d54d743fc5a0819301e06c633
https://europepmc.org/articles/PMC52564/

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Partial amino acid sequence of human thrombospondin as determined by analysis of cDNA clones: homology to malarial circumsporozoite proteins

Autor: Paul Framson, Paul Bornstein, Sentaro Kobayashi, Francine Eden-McCutchan

Publikováno v: Biochemistry. 25(26)

A lambda gt 11 library prepared from human umbilical vein endothelial cell RNA was screened for cDNAs encoding thrombospondin. Reagents included a monospecific antibody to human thrombospondin and a mixture of four synthetic oligodeoxyribonucleotides

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74fbeaacba8c410e81c9c70d3e891af3
https://pubmed.ncbi.nlm.nih.gov/3030396

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