The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation.

Autor: Lydia M Castelli, David Talavera, Christopher J Kershaw, Sarah S Mohammad-Qureshi, Joseph L Costello, William Rowe, Paul F G Sims, Christopher M Grant, Simon J Hubbard, Mark P Ashe, Graham D Pavitt

Publikováno v: PLoS Genetics, Vol 11, Iss 5, p e1005233 (2015)

Translation initiation factor eIF4E mediates mRNA selection for protein synthesis via the mRNA 5'cap. A family of binding proteins, termed the 4E-BPs, interact with eIF4E to hinder ribosome recruitment. Mechanisms underlying mRNA specificity for 4E-B

Externí odkaz: https://doaj.org/article/4442571fc9da4cb6b3f3487cf8c78944

Archetypal transcriptional blocks underpin yeast gene regulation in response to changes in growth conditions

Autor: David Talavera, Christopher J. Kershaw, Joseph L. Costello, Lydia M. Castelli, William Rowe, Paul F. G. Sims, Mark P. Ashe, Chris M. Grant, Graham D. Pavitt, Simon J. Hubbard

Publikováno v: Talavera, D, Kershaw, C, Costello, J L, Castelli, L M, Rowe, W, Sims, P F G, Ashe, M, Grant, C, Pavitt, G D & Hubbard, S 2018, ' Archetypal transcriptional blocks underpin yeast gene regulation in response to changes in growth conditions ', Scientific Reports, vol. 8, 7949, pp. 7949 . https://doi.org/10.1038/s41598-018-26170-5
Scientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)

The transcriptional responses of yeast cells to diverse stresses typically include gene activation and repression. Specific stress defense, citric acid cycle and oxidative phosphorylation genes are activated, whereas protein synthesis genes are coord

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::f909c8cab9f3eaf594d553ae609631ec
https://research.manchester.ac.uk/en/publications/366ee6a6-1eed-445a-8035-9f372affe983

Puf3p induces translational repression of genes linked to oxidative stress

Autor: Paul F. G. Sims, Graham D. Pavitt, Mark P. Ashe, William Rowe, Lydia M. Castelli, Joseph L. Costello, Chris M. Grant, Christopher J. Kershaw, Simon J. Hubbard

Publikováno v: Nucleic Acids Research

In response to stress, the translation of many mRNAs in yeast can change in a fashion discordant with the general repression of translation. Here, we use machine learning to mine the properties of these mRNAs to determine specific translation control

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c162687eb7c50e95689953056caf93ee
http://europepmc.org/articles/PMC3902938

Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring

Autor: Karin Lanthaler, Chris M. Grant, Victoria M. Harman, Paul F. G. Sims, Simon J. Hubbard, Robert J. Beynon, Craig Lawless, Dean E. Hammond, Rachel H. Watkins, Rebecca L. Miller, Claire E. Eyers, Philip Brownridge, Stephen W. Holman

Publikováno v: Molecular and Cellular Proteomics
Lawless, C, Holman, S W, Brownridge, P, Lanthaler, K, Harman, V M, Watkins, R, Hammond, D E, Miller, R L, Sims, P F G, Grant, C, Eyers, C E, Beynon, R J & Hubbard, S 2016, ' Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring. ', Molecular and Cellular Proteomics, vol. 15, no. 4, pp. 1309-1322 . https://doi.org/10.1074/mcp.M115.054288
Molecular & Cellular Proteomics : MCP

Defining intracellular protein concentration is critical in molecular systems biology. Although strategies for determining relative protein changes are available, defining robust absolute values in copies per cell has proven significantly more challe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e08430dd8363cdb7f183992c61a8a44f

Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated

Autor: Jonathon Bone, Julian N. Selley, Mark P. Ashe, Jennifer Lui, Nathaniel P. Hoyle, Lydia M. Castelli, Paul F. G. Sims, Susan G. Campbell, William Rowe, Leah E. A. Holmes, Leo A. H. Zeef

Publikováno v: Molecular Biology of the Cell

The mechanism and consequences of the translational inhibition caused by glucose depletion in yeast are characterized. eIF4A is lost from the preinitiation complex, and the pentose phosphate pathway is translationally up-regulated, allowing an effici

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9f383e31ab60510c64efa60429a0ace
https://doi.org/10.1091/mbc.e11-02-0153