Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Paul E. Nisson"'
Publikováno v:
Molecular and Cellular Biology. 19:3696-3703
Random homozygous knockout (RHKO) is an antisense RNA strategy capable of identifying genes whose homozygous functional inactivation yields a selectable phenotype in cells growing in culture. Using this approach, we isolated NIH 3T3 fibroblast clones
Autor:
John J. Kleiderlein, Paul E. Nisson, Joel Jessee, W.-B. Li, K. G. Becker, Michael L. Derby, Christopher A. Ross, R. L. Margolis
Publikováno v:
Human Genetics. 103:666-673
Expansion mutations of trinucleotide repeats and other units of unstable DNA have been proposed to account for at least some of the genetic susceptibility to a number of neuropsychiatric disorders, including bipolar affective disorder, schizophrenia,
Autor:
Paul C. Watkins, Fabrizia Faustinella, Anne Hagemeijer, Nicoletta Sacchi, Paul E. Nisson, J. Wijsman
Publikováno v:
Genes, Chromosomes and Cancer. 11:226-236
The t(3;21)(q26;q22) is associated with chronic myelogenous leukemia in blast crisis (CML-BC), leukemia evolving from (therapy-related) myelodysplasia, and with leukemia following other hematopoietic proliferative diseases. Molecular cytogenetic anal
Publikováno v:
Genome Research. 4:S24-S39
Publikováno v:
Analytical Biochemistry. 212:428-435
A method for efficient nonradioactive labeling of DNA with biotin using random primer extension has been developed. Under the conditions described, a significant amount of DNA synthesis occurs during incorporation of the nonradioactive label, resulti
Publikováno v:
Development. 114:193-201
The expression of the Spec1 gene of Strongylocentrotus purpuratus and its Lytechinus pictus homologue LpS1 was analyzed in reciprocal hybrid embryos of these two species of sea urchin. While the time course of accumulation of Spec1 mRNA was nearly no
Publikováno v:
Developmental Biology. 144:405-411
Hybrid embryos were derived from reciprocal crosses of Strongylocentrotus purpuratus and Lytechinus pictus sea urchins. The expression of proteins specific for L. pictus was restricted in these hybrid embryos, while this was not so for most proteins
Publikováno v:
Current Protocols in Molecular Biology
It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high-quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficienc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e182ac1fbf5438a132ee6d8654eddfcc
https://doi.org/10.1002/0471142727.mb1504s56
https://doi.org/10.1002/0471142727.mb1504s56
Publikováno v:
The Journal of biological chemistry. 275(51)
Sam68 is a multimeric 68-kDa RNA-binding nuclear protein of unknown function that interacts with, and is tyrosine-phosphorylated by, the oncogenic protein Src during mitosis. Random homozygous knock-out (RHKO) is a retroviral-based antisense RNA stra
Autor:
Christopher W. Lawrence, Veronica M. Maher, Peter E. M. Gibbs, W. Glenn McGregor, Paul E. Nisson
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 95(12)
To get a better understanding of mutagenic mechanisms in humans, we have cloned and sequenced the human homolog of the Saccharomyces cerevisiae REV3 gene. The yeast gene encodes the catalytic subunit of DNA polymerase ζ, a nonessential enzyme that i