Zobrazeno 1 - 10
of 56
pro vyhledávání: '"Paul B. Bennett"'
Publikováno v:
Analytical Biochemistry. 327:74-81
The current studies describe a new, robust cell-based functional assay useful to characterize L-type voltage-dependent calcium channels and their antagonists. The basis of this assay is measurement in plate format of Ca2+ influx through the L-type Ca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::336c2ea8494948bd090b6069518b3be5
https://doi.org/10.1016/j.ab.2004.01.003
https://doi.org/10.1016/j.ab.2004.01.003
Generation and Characterization of a Cell Line with Inducible Expression of Cav3.2 (T-Type) Channels
Autor:
Menghang Xia, John Imredy, Kenneth S. Koblan, Vincent P. Santarelli, Cindra L. Condra, Hongyu Annie Liang, Paul B. Bennett, Thomas M. Connolly
Publikováno v:
ASSAY and Drug Development Technologies. 1:637-645
Establishment of stable cell lines that constitutively express Ca(2+) channels at high density and that are useful for in vitro studies may be complicated by problems with seal quality and duration during whole-cell patch-clamp electrophysiology. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0365844c9d0f92b832f0a1a7b8afc18
https://doi.org/10.1089/154065803770380995
https://doi.org/10.1089/154065803770380995
Publikováno v:
Circulation Research. 93:491-499
Most antiarrhythmic drugs are ion channel blockers, and to date, those tested in large randomized placebo-controlled clinical trials have shown no decrease in mortality outcome. This apparent lack of survival benefit may result from the significant l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13dcf05e8673255d60f242d0a33efaed
https://doi.org/10.1161/01.res.0000091829.63501.a8
https://doi.org/10.1161/01.res.0000091829.63501.a8
Autor:
Kenneth S. Koblan, Victor N. Uebele, Kirk S. Schroeder, Paul B. Bennett, Laszlo Kiss, Brad Neagle, Stefanie A. Kane
Publikováno v:
ASSAY and Drug Development Technologies. 1:127-135
Technological advances often drive major breakthroughs in biology. Examples include PCR, automated DNA sequencing, confocal/single photon microscopy, AFM, and voltage/patch-clamp methods. The patch-clamp method, first described nearly 30 years ago, w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bedf40155f41c914acd09a3c5a75666
https://doi.org/10.1089/154065803321537845
https://doi.org/10.1089/154065803321537845
Autor:
Jerzy Karczewski, Kimberly Della Penna, Jixin Wang, Joseph J. Salata, Thomas M. Connolly, Hao Wang, Kenneth S. Koblan, Paul B. Bennett
Publikováno v:
American Journal of Physiology-Heart and Circulatory Physiology. 284:H256-H267
We established HEK-293 cell lines that stably express functional canine ether-à-go-go-related gene (cERG) K+ channels and examined their biophysical and pharmacological properties with whole cell patch clamp and35S-labeled MK-499 ([35S]MK-499) bindi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2feb88e31ba8918ec7e282c8814e441
https://doi.org/10.1152/ajpheart.00220.2002
https://doi.org/10.1152/ajpheart.00220.2002
Publikováno v:
The Journal of General Physiology
Potassium (K+) channels mediate numerous electrical events in excitable cells, including cellular membrane potential repolarization. The hERG K+ channel plays an important role in myocardial repolarization, and inhibition of these K+ channels is asso
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf8e01e4c6b5ea4c288fa33cbfa2130a
http://europepmc.org/articles/PMC2217357
http://europepmc.org/articles/PMC2217357
Publikováno v:
The Journal of Neuroscience. 21:4143-4153
Potassium (K+) channels influence neurotransmitter release, burst firing rate activity, pacing, and critical dampening of neuronal circuits. Internal and external factors that further modify K+channel function permit fine-tuning of neuronal circuits.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::340cf3af802a36ba8af951bd7e22de85
https://doi.org/10.1523/jneurosci.21-12-04143.2001
https://doi.org/10.1523/jneurosci.21-12-04143.2001
Autor:
Yuan Liu, Paul B. Bennett, Edward McKenna, David J. Figueroa, Armando Lagrutta, Theresa Wade, Victor N. Uebele, Christopher P. Austin, Richard Swanson
Publikováno v:
Journal of Biological Chemistry. 275:23211-23218
We report here a characterization of two families of calcium-activated K(+) channel beta-subunits, beta2 and beta3, which are encoded by distinct genes that map to 3q26.2-27. A single beta2 family member and four alternatively spliced variants of bet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::365971e9dd68f0eb21d1d9f0f2c7a082
https://doi.org/10.1074/jbc.m910187199
https://doi.org/10.1074/jbc.m910187199
Publikováno v:
Biophysical Journal. 77:2534-2541
We have studied the functional effects of extracellular Cd(2+) on human ether-a-go-go-related gene (HERG) encoded K(+) channels. Low concentrations (10-200 microM) of extracellular Cd(2+) increased outward currents through HERG channels; 200 microM C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a94239c65c342da5e14587f02845abe
https://doi.org/10.1016/s0006-3495(99)77088-8
https://doi.org/10.1016/s0006-3495(99)77088-8
Publikováno v:
Journal of General Physiology. 113:565-580
Human ether-à-go-go–related gene (HERG) encoded K+ channels were expressed in Chinese hamster ovary (CHO-K1) cells and studied by whole-cell voltage clamp in the presence of varied extracellular Ca2+ concentrations and physiological external K+. E
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0b89957c83f2165dbd244ef8f4689b42
https://doi.org/10.1085/jgp.113.4.565
https://doi.org/10.1085/jgp.113.4.565