Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue

Autor: Simon A. Hardwick, Wen Hu, Anoushka Joglekar, Li Fan, Paul G. Collier, Careen Foord, Jennifer Balacco, Samantha Lanjewar, Maureen McGuirk Sampson, Frank Koopmans, Andrey D. Prjibelski, Alla Mikheenko, Natan Belchikov, Julien Jarroux, Anne Bergstrom Lucas, Miklós Palkovits, Wenjie Luo, Teresa A. Milner, Lishomwa C. Ndhlovu, August B. Smit, John Q. Trojanowski, Virginia M. Y. Lee, Olivier Fedrigo, Steven A. Sloan, Dóra Tombácz, M. Elizabeth Ross, Erich Jarvis, Zsolt Boldogkői, Li Gan, Hagen U. Tilgner

Publikováno v: Nature Biotechnology, 40(7), 1082-1092. Nature Publishing Group
Hardwick, S A, Hu, W, Joglekar, A, Fan, L, Collier, P G, Foord, C, Balacco, J, Lanjewar, S, Sampson, M M G, Koopmans, F, Prjibelski, A D, Mikheenko, A, Belchikov, N, Jarroux, J, Lucas, A B, Palkovits, M, Luo, W, Milner, T A, Ndhlovu, L C, Smit, A B, Trojanowski, J Q, Lee, V M Y, Fedrigo, O, Sloan, S A, Tombácz, D, Ross, M E, Jarvis, E, Boldogkői, Z, Gan, L & Tilgner, H U 2022, ' Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue ', Nature Biotechnology, vol. 40, no. 7, pp. 1082-1092 . https://doi.org/10.1038/s41587-022-01231-3

Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19f086b2331c75eade283b32db9e2f0b
https://hdl.handle.net/1871.1/133d60c1-4a56-4cd6-98f3-fbc172b0ebeb

Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across human brain cell types

Autor: Simon A Hardwick, Wen Hu, Anoushka Joglekar, Li Fan, Paul G Collier, Careen Foord, Jennifer Balacco, Natan Belchikov, Julien Jarroux, Andrey Prjibelski, Alla Mikheenko, Wenjie Luo, Teresa A Milner, Lishomwa C Ndhlovu, John Q Trojanowski, Virginia MY Lee, Olivier Fedrigo, Dóra Tombácz, M Elizabeth Ross, Erich Jarvis, Zsolt Boldogkői, Li Gan, Hagen U Tilgner

Single-nuclei RNA-Seq is being widely employed to investigate cell types, especially of human brain and other frozen samples. In contrast to single-cell approaches, however, the majority of single-nuclei RNA counts originate from partially processed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b222d64c0715dba7ed65004bff3e007f
https://doi.org/10.1101/2021.12.29.474385

Prevention and treatment of dilator injuries during central venous catheter placement

Autor: Paul E. Collier

Publikováno v: Journal of Vascular Surgery: Venous and Lymphatic Disorders. 7:789-792

Backround Misuse of vascular dilators during the placement of central venous catheters has been infrequently reported and can lead to devastating intrathoracic hemorrhage and death. These injuries should be preventable in most cases. If a major intra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a08c049ba131acffe58068c239972a57
https://doi.org/10.1016/j.jvsv.2019.06.020

A study of housing conditions in a small city

Autor: Paul Stanley Collier

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5e1627743ce11254c05fac8a5904ee48
https://doi.org/10.17077/etd.agh6ddrj

Delayed Diagnosis of Popliteal Artery Injury After Total Knee Replacement

Autor: Timothy Hanlon, Paul E. Collier

Publikováno v: Journal of Vascular Surgery. 70:e15

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::00728773658197a943b7e077eb4c7ce4
https://doi.org/10.1016/j.jvs.2019.06.004