Zobrazeno 1 - 10
of 146
pro vyhledávání: '"Paul A, Dalby"'
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-14 (2024)
Abstract We have previously engineered E. coli transketolase (TK) enzyme variants that accept new substrates such as aliphatic or aromatic aldehydes, and also with improved thermal stability. Irreversible aggregation is the primary mechanism of deact
Externí odkaz:
https://doaj.org/article/e4903fbb168443deaac0d7082ae92347
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)
Abstract Improving the range of substrates accepted by enzymes with high catalytic activity remains an important goal for the industrialisation of biocatalysis. Many enzymes catalyse two-substrate reactions which increases the complexity in engineeri
Externí odkaz:
https://doaj.org/article/db466c2301ad4b5e8781debbca32e1e1
Autor:
Jiazhi Tang, Cheng Zhang, Nuria Codina Castillo, Christophe J. Lalaurie, Xin Gao, Paul A. Dalby, Frank Kozielski
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-14 (2023)
Abstract Antibody-fragment (Fab) therapy development has the potential to be accelerated by computational modelling and simulations that predict their target binding, stability, formulation, manufacturability, and the impact of further protein engine
Externí odkaz:
https://doaj.org/article/821ca3cb011b454cb4dbf4b1591c569d
Publikováno v:
npj Vaccines, Vol 8, Iss 1, Pp 1-9 (2023)
Abstract Distribution of vaccines which require refrigerated or frozen storage can be challenging and expensive. The adenovirus vector platform has been widely used for COVID-19 vaccines while several further candidate vaccines using the platform are
Externí odkaz:
https://doaj.org/article/4ebe90e713b3436f9874cb854ddf0a4f
Autor:
Valentina A. Spiteri, James Doutch, Robert P. Rambo, Jayesh S. Bhatt, Jayesh Gor, Paul A. Dalby, Stephen J. Perkins
Publikováno v:
PLoS ONE, Vol 19, Iss 4 (2024)
Externí odkaz:
https://doaj.org/article/a02df80ff0bb46bcb079f2c6bf76cc4b
Autor:
Weiluo Lee, Subhas Pradhan, Cheng Zhang, Niccolo A. E. Venanzi, Weina Li, Stephen Goldrick, Paul A. Dalby
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-17 (2022)
Abstract Bovine enterokinase light chain (EKL) is an industrially useful protease for accurate removal of affinity-purification tags from high-value biopharmaceuticals. However, recombinant expression in Escherichia coli produces insoluble inclusion
Externí odkaz:
https://doaj.org/article/c73df12f10b54e7cba3c138d6b532ca1
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Abstract Transketolase (TK) has been previously engineered, using semi-rational directed evolution and substrate walking, to accept increasingly aliphatic, cyclic, and then aromatic substrates. This has ultimately led to the poor water solubility of
Externí odkaz:
https://doaj.org/article/626886870fd2490fb56a25f8d836e987
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 19, Iss , Pp 2750-2760 (2021)
Conformational stability of a protein is usually obtained by spectroscopically measuring the unfolding melting temperature. However, optical spectra under native conditions are considered to contain too little resolution to probe protein stability. H
Externí odkaz:
https://doaj.org/article/1aa9c8be4fa84d1aa1767f52fd44cfd3
Autor:
Cheng Zhang, Nuria Codina, Jiazhi Tang, Haoran Yu, Nesrine Chakroun, Frank Kozielski, Paul A. Dalby
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 19, Iss , Pp 2726-2741 (2021)
Successful development of protein therapeutics depends critically on achieving stability under a range of conditions. A deeper understanding of the drivers of instability across different stress conditions, will enable the engineering of more robust
Externí odkaz:
https://doaj.org/article/b59a28092c0f416f8bb210089774ee83
Autor:
Pierre-Yves Colin, Paul A. Dalby
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Abstract RNA-based drugs are an emerging class of therapeutics combining the immense potential of DNA gene-therapy with the absence of genome integration-associated risks. While the synthesis of such molecules is feasible, large scale in vitro produc
Externí odkaz:
https://doaj.org/article/2f9517bf037f477d8cdc334243cc1253